Profiling the iron, copper and zinc content in primary neuron and astrocyte cultures by rapid online quantitative size exclusion chromatography-inductively coupled plasma-mass spectrometry

Dominic J Hare, Alexandra Grubman, Timothy M Ryan, Amber Lothian, Jeffrey R. Liddell, Rudolf Grimm, Toshiaki Matsude, Philip Doble, Robert A Cherny, Ashley I. Bush, Anthony R White, Colin L. Masters, Blaine R. Roberts

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Metals often determine the chemical reactivity of the proteins to which they are bound. Each cell in the body tightly maintains a unique metalloproteomic profile, mostly dependent on function. This paper describes an analytical online flow injection quantitative size exclusion chromatography-inductively coupled plasma-mass spectrometry (SEC-ICP-MS) method, which was applied to profiling the metal-binding proteins found in primary cultures of neurons and astrocytes. This method can be conducted using similar amounts of sample to those used for Western blotting (20-150 μg protein), and has a turnaround time of -1). Total on-column Fe, Cu and Zn detection limits ranged from 0.825 ± 0.005 ng to 13.6 ± 0.7 pg. Neurons and astrocytes exhibited distinct metalloprotein profiles, featuring both ubiquitous and unique metalloprotein species. Separation and detection by SEC-ICP-MS allows appraisal of these metalloproteins in their native state, and online quantification was achieved using this relatively simple external calibration process.
Original languageEnglish
Pages (from-to)1656-1662
Number of pages7
JournalMetallomics
Volume5
Issue number12
DOIs
Publication statusPublished - Dec 2013
Externally publishedYes

Cite this

Hare, Dominic J ; Grubman, Alexandra ; Ryan, Timothy M ; Lothian, Amber ; Liddell, Jeffrey R. ; Grimm, Rudolf ; Matsude, Toshiaki ; Doble, Philip ; Cherny, Robert A ; Bush, Ashley I. ; White, Anthony R ; Masters, Colin L. ; Roberts, Blaine R. / Profiling the iron, copper and zinc content in primary neuron and astrocyte cultures by rapid online quantitative size exclusion chromatography-inductively coupled plasma-mass spectrometry. In: Metallomics. 2013 ; Vol. 5, No. 12. pp. 1656-1662.
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title = "Profiling the iron, copper and zinc content in primary neuron and astrocyte cultures by rapid online quantitative size exclusion chromatography-inductively coupled plasma-mass spectrometry",
abstract = "Metals often determine the chemical reactivity of the proteins to which they are bound. Each cell in the body tightly maintains a unique metalloproteomic profile, mostly dependent on function. This paper describes an analytical online flow injection quantitative size exclusion chromatography-inductively coupled plasma-mass spectrometry (SEC-ICP-MS) method, which was applied to profiling the metal-binding proteins found in primary cultures of neurons and astrocytes. This method can be conducted using similar amounts of sample to those used for Western blotting (20-150 μg protein), and has a turnaround time of -1). Total on-column Fe, Cu and Zn detection limits ranged from 0.825 ± 0.005 ng to 13.6 ± 0.7 pg. Neurons and astrocytes exhibited distinct metalloprotein profiles, featuring both ubiquitous and unique metalloprotein species. Separation and detection by SEC-ICP-MS allows appraisal of these metalloproteins in their native state, and online quantification was achieved using this relatively simple external calibration process.",
author = "Hare, {Dominic J} and Alexandra Grubman and Ryan, {Timothy M} and Amber Lothian and Liddell, {Jeffrey R.} and Rudolf Grimm and Toshiaki Matsude and Philip Doble and Cherny, {Robert A} and Bush, {Ashley I.} and White, {Anthony R} and Masters, {Colin L.} and Roberts, {Blaine R.}",
year = "2013",
month = "12",
doi = "10.1039/c3mt00227f",
language = "English",
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pages = "1656--1662",
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Hare, DJ, Grubman, A, Ryan, TM, Lothian, A, Liddell, JR, Grimm, R, Matsude, T, Doble, P, Cherny, RA, Bush, AI, White, AR, Masters, CL & Roberts, BR 2013, 'Profiling the iron, copper and zinc content in primary neuron and astrocyte cultures by rapid online quantitative size exclusion chromatography-inductively coupled plasma-mass spectrometry' Metallomics, vol. 5, no. 12, pp. 1656-1662. https://doi.org/10.1039/c3mt00227f

Profiling the iron, copper and zinc content in primary neuron and astrocyte cultures by rapid online quantitative size exclusion chromatography-inductively coupled plasma-mass spectrometry. / Hare, Dominic J; Grubman, Alexandra; Ryan, Timothy M; Lothian, Amber; Liddell, Jeffrey R.; Grimm, Rudolf; Matsude, Toshiaki; Doble, Philip; Cherny, Robert A; Bush, Ashley I.; White, Anthony R; Masters, Colin L.; Roberts, Blaine R.

In: Metallomics, Vol. 5, No. 12, 12.2013, p. 1656-1662.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Lothian, Amber

AU - Liddell, Jeffrey R.

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AU - Matsude, Toshiaki

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AU - Cherny, Robert A

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AU - White, Anthony R

AU - Masters, Colin L.

AU - Roberts, Blaine R.

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