TY - JOUR
T1 - Profiling of potential antibacterial compounds of lactic acid bacteria against Extremely Drug Resistant (XDR) acinetobacter baumannii
AU - Yap, Phui Chyng
AU - Ayuhan, Noorfazlin
AU - Woon, Jia Jie
AU - Teh, Cindy Shuan Ju
AU - Lee, Vannajan Sanghiran
AU - Azman, Adzzie Shazleen
AU - AbuBakar, Sazaly
AU - Lee, Hai Yen
PY - 2021/3/19
Y1 - 2021/3/19
N2 - A total of 20 of isolates of lactic acid bacteria (LAB) were selected and screened for antagonistic activity against clinical strains of 30 clinical isolates of extremely drug-resistant (XDR) Acinetobacter baumannii using the well diffusion assay method. Results showed that 50% of the highly LAB strains possessed inhibitory activity against (up to 66%) of the XDR A. baumannii strains tested. The supernatant of the twenty LAB strains was subjected to gas chromatography mass spectrometry (GCMS) revealed that the common compound found in the active isolates against XDR A. baumannii was 3-Isobutyl-2,3,6,7,8,8a-hexahydropyrrolo[1,2-a]pyrazine-1,4-dione, a known potential diketopiperazine group. The molecular docking study against potential antibacterial targets with selected ligands was performed to predict the binding mode of interactions, which is responsible for antibacterial activity. The docking analysis of the potent compounds supported the potential antibacterial activity exhibiting high inhibition constant and binding affinity in silico.
AB - A total of 20 of isolates of lactic acid bacteria (LAB) were selected and screened for antagonistic activity against clinical strains of 30 clinical isolates of extremely drug-resistant (XDR) Acinetobacter baumannii using the well diffusion assay method. Results showed that 50% of the highly LAB strains possessed inhibitory activity against (up to 66%) of the XDR A. baumannii strains tested. The supernatant of the twenty LAB strains was subjected to gas chromatography mass spectrometry (GCMS) revealed that the common compound found in the active isolates against XDR A. baumannii was 3-Isobutyl-2,3,6,7,8,8a-hexahydropyrrolo[1,2-a]pyrazine-1,4-dione, a known potential diketopiperazine group. The molecular docking study against potential antibacterial targets with selected ligands was performed to predict the binding mode of interactions, which is responsible for antibacterial activity. The docking analysis of the potent compounds supported the potential antibacterial activity exhibiting high inhibition constant and binding affinity in silico.
KW - antimicrobial resistance (AMR)
KW - diketopiperazine (DKP)
KW - infectious diseases
KW - lactic acid bacteria
KW - Malaysia
UR - http://www.scopus.com/inward/record.url?scp=85103863019&partnerID=8YFLogxK
U2 - 10.3390/molecules26061727
DO - 10.3390/molecules26061727
M3 - Article
C2 - 33808805
AN - SCOPUS:85103863019
SN - 1420-3049
VL - 26
JO - Molecules
JF - Molecules
IS - 6
M1 - 1727
ER -
