Profiling of potential antibacterial compounds of lactic acid bacteria against Extremely Drug Resistant (XDR) acinetobacter baumannii

Phui Chyng Yap, Noorfazlin Ayuhan, Jia Jie Woon, Cindy Shuan Ju Teh, Vannajan Sanghiran Lee, Adzzie Shazleen Azman, Sazaly AbuBakar, Hai Yen Lee

    Research output: Contribution to journalArticleResearchpeer-review

    Abstract

    A total of 20 of isolates of lactic acid bacteria (LAB) were selected and screened for antagonistic activity against clinical strains of 30 clinical isolates of extremely drug-resistant (XDR) Acinetobacter baumannii using the well diffusion assay method. Results showed that 50% of the highly LAB strains possessed inhibitory activity against (up to 66%) of the XDR A. baumannii strains tested. The supernatant of the twenty LAB strains was subjected to gas chromatography mass spectrometry (GCMS) revealed that the common compound found in the active isolates against XDR A. baumannii was 3-Isobutyl-2,3,6,7,8,8a-hexahydropyrrolo[1,2-a]pyrazine-1,4-dione, a known potential diketopiperazine group. The molecular docking study against potential antibacterial targets with selected ligands was performed to predict the binding mode of interactions, which is responsible for antibacterial activity. The docking analysis of the potent compounds supported the potential antibacterial activity exhibiting high inhibition constant and binding affinity in silico.

    Original languageEnglish
    Article number1727
    Number of pages14
    JournalMolecules
    Volume26
    Issue number6
    DOIs
    Publication statusPublished - 19 Mar 2021

    Keywords

    • antimicrobial resistance (AMR)
    • diketopiperazine (DKP)
    • infectious diseases
    • lactic acid bacteria
    • Malaysia

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