Production of the matrix protein of Nipah virus in Escherichia coli: virus-like particles and possible application for diagnosis

Senthil Kumar Subramanian, Beng Ti Tey, Muhajir Hamid, Wen Siang Tan

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Abstract

The broad species tropism of Nipah virus (NiV) coupled with its high pathogenicity demand a rapid search for a new biomarker candidate for diagnosis. The matrix (M) protein was expressed in Escherichia coli and purified using a Ni-NTA affinity column chromatography and sucrose density gradient centrifugation. The recombinant M protein with the molecular mass (Mr) of about 43 kDa was detected by anti-NiV serum and anti-myc antibody. About 50% of the M protein was found to be soluble and localized in cytoplasm when the cells were grown at 30 °C. Electron microscopic analysis showed that the purified M protein assembled into spherical particles of different sizes with diameters ranging from 20 to 50 nm. The purified M protein showed significant reactivity with the swine sera collected during the NiV outbreak, demonstrating its potential as a diagnostic reagent.

Original languageEnglish
Pages (from-to)179-183
Number of pages5
JournalJournal of Virological Methods
Volume162
Issue number1-2
DOIs
Publication statusPublished - Jan 2009
Externally publishedYes

Keywords

  • Escherichia coli
  • Matrix protein
  • Nipah virus
  • Paramyxovirus
  • Virus-like particles

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