Production of interferons by dendritic cells, plasmacytoid cells, natural killer cells, and interferon-producing killer dendritic cells

David Vremec, Meredith O'Keeffe, Hubertus Hochrein, Martina Fuchsberger, Irina Caminschi, Mireille Lahoud, Ken Shortman

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102 Citations (Scopus)

Abstract

The capacity of mouse spleen conventional dendritic cells (cDCs) and plasmacytoid dendritic cells (pDCs) to produce interferon-γ (IFN-γ) or IFN-α was assessed, and compared with that of natural killer (NK) cells and the recently identified interferon-producing killer dendritic cells (IKDCs), both of which are frequent contaminants in DC preparations. Fully developed cDCs or pDCs, if free of NK cells or IKDCs, showed little capacity for IFN-γ production. However, an early developmental form of the CD4 -8+ cDC subtype, and the Ly6C- Ly49Q - pDC subtype, both were able to produce moderate amounts of IFN-γ, although less than IKDCs. In response to toll-like receptor 9 stimuli, both the Ly6C+ Ly49Q+ and the Ly6C- Ly49Q- pDC subtypes were effective producers of IFN-α. However, IKDCs, which efficiently produced IFN-γ and showed immediate cytotoxicity on NK target cells, did not produce IFN-α under these conditions.

Original languageEnglish
Pages (from-to)1165-1173
Number of pages9
JournalBlood
Volume109
Issue number3
DOIs
Publication statusPublished - 1 Feb 2007
Externally publishedYes

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