@inbook{d2953b28e0b0474db6e29d7107f60c77,
title = "Production of high-titer lentiviral particles for stable genetic modification of mammalian cells",
abstract = "Lentiviral gene transfer technologies exploit the natural efficiency of viral transduction to integrate exogenous genes into mammalian cells. This provides a simple research tool for inducing transgene expression or endogenous gene knockdown in both dividing and nondividing cells. This chapter describes an improved protocol for polyethylenimine (PEI)-mediated multi-plasmid transfection and polyethylene glycol (PEG) precipitation to generate and concentrate lentiviral vectors.",
keywords = "Gene transfer, PEG precipitation, PEI transfection, Titration",
author = "Larcombe, {Michael R.} and Jan Manent and Joseph Chen and Ketan Mishra and Xiaodong Liu and Nefzger, {Christian M.}",
year = "2019",
month = jan,
day = "1",
doi = "10.1007/978-1-4939-9086-3_4",
language = "English",
isbn = "9781493990856",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "47--61",
editor = "Ivan Bertoncello",
booktitle = "Mouse Cell Culture",
address = "United States of America",
}