Production of hepatitis B core antigen in a stirred tank bioreactor: the influence of temperature and agitation

Beng Ti Tey; Mung Ing Chua; Ghee Sung Chua; Michelle Yeen Tan Ng; Dayang Radiah Awang Biak; Wen Siang Tan; Tau Chuan Ling

doi:10.1007/BF02931902

Production of hepatitis B core antigen in a stirred tank bioreactor: the influence of temperature and agitation

Beng Ti Tey
, Mung Ing Chua
, Ghee Sung Chua
, Michelle Yeen Tan Ng
, Dayang Radiah Awang Biak
, Wen Siang Tan
, Tau Chuan Ling

Research output: Contribution to journal › Article › Research › peer-review

2 Citations (Scopus)

Abstract

The influence of temperature and agitation on the growth of Escherichia coli expressing hepatitis B core antigen (HBcAg) in stirred tank bioreactor were investigated. The highest specific growth rate for E. coli (0.844 h^-1) was achieved at a temperature of 37°C and an agitation speed of 250 rpm. The activation energy for the growth of the E. coli strain W31101Q in the stirred tank bioreactor was estimated to be 11 kcal/mol. The highest protein yield was achieved at a temperature of 44°C and an agitation speed of 250 rpm. The relative protein concentration at 44°C is 30 and 6% higher compared to that at 30 and 37°C, respectively.

Original language	English
Pages (from-to)	164-167
Number of pages	4
Journal	Biotechnology and Bioprocess Engineering
Volume	11
Issue number	2
DOIs	https://doi.org/10.1007/BF02931902
Publication status	Published - Mar 2006
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Agitation speed
Bioreactor
E. coli
HBcAg
Temperature

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10.1007/BF02931902

Cite this

@article{27164a5b79d84146a70a03bebcf3d38e,

title = "Production of hepatitis B core antigen in a stirred tank bioreactor: the influence of temperature and agitation",

abstract = "The influence of temperature and agitation on the growth of Escherichia coli expressing hepatitis B core antigen (HBcAg) in stirred tank bioreactor were investigated. The highest specific growth rate for E. coli (0.844 h-1) was achieved at a temperature of 37°C and an agitation speed of 250 rpm. The activation energy for the growth of the E. coli strain W31101Q in the stirred tank bioreactor was estimated to be 11 kcal/mol. The highest protein yield was achieved at a temperature of 44°C and an agitation speed of 250 rpm. The relative protein concentration at 44°C is 30 and 6\% higher compared to that at 30 and 37°C, respectively.",

keywords = "Agitation speed, Bioreactor, E. coli, HBcAg, Temperature",

author = "Tey, \{Beng Ti\} and Chua, \{Mung Ing\} and Chua, \{Ghee Sung\} and \{Tan Ng\}, \{Michelle Yeen\} and \{Awang Biak\}, \{Dayang Radiah\} and Tan, \{Wen Siang\} and Ling, \{Tau Chuan\}",

note = "Funding Information: \textasciicircum{}{\AA}{\^a}{\aa}{\c c}{\"i}{\"a}{\'E}{\c C}{\"O}{\'E}{\~a}{\'E}{\aa}{\'i} This study was supported by an IRPA Grant (09-02-04-0766-EA001) from the Ministry of Science, Technology, and Innovation, Malaysia. Copyright: Copyright 2009 Elsevier B.V., All rights reserved.",

year = "2006",

month = mar,

doi = "10.1007/BF02931902",

language = "English",

volume = "11",

pages = "164--167",

journal = "Biotechnology and Bioprocess Engineering",

issn = "1226-8372",

publisher = "Springer-Verlag London Ltd.",

number = "2",

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TY - JOUR

T1 - Production of hepatitis B core antigen in a stirred tank bioreactor

T2 - the influence of temperature and agitation

AU - Tey, Beng Ti

AU - Chua, Mung Ing

AU - Chua, Ghee Sung

AU - Tan Ng, Michelle Yeen

AU - Awang Biak, Dayang Radiah

AU - Tan, Wen Siang

AU - Ling, Tau Chuan

N1 - Funding Information: ^ÅâåçïäÉÇÖÉãÉåí This study was supported by an IRPA Grant (09-02-04-0766-EA001) from the Ministry of Science, Technology, and Innovation, Malaysia. Copyright: Copyright 2009 Elsevier B.V., All rights reserved.

PY - 2006/3

Y1 - 2006/3

N2 - The influence of temperature and agitation on the growth of Escherichia coli expressing hepatitis B core antigen (HBcAg) in stirred tank bioreactor were investigated. The highest specific growth rate for E. coli (0.844 h-1) was achieved at a temperature of 37°C and an agitation speed of 250 rpm. The activation energy for the growth of the E. coli strain W31101Q in the stirred tank bioreactor was estimated to be 11 kcal/mol. The highest protein yield was achieved at a temperature of 44°C and an agitation speed of 250 rpm. The relative protein concentration at 44°C is 30 and 6% higher compared to that at 30 and 37°C, respectively.

AB - The influence of temperature and agitation on the growth of Escherichia coli expressing hepatitis B core antigen (HBcAg) in stirred tank bioreactor were investigated. The highest specific growth rate for E. coli (0.844 h-1) was achieved at a temperature of 37°C and an agitation speed of 250 rpm. The activation energy for the growth of the E. coli strain W31101Q in the stirred tank bioreactor was estimated to be 11 kcal/mol. The highest protein yield was achieved at a temperature of 44°C and an agitation speed of 250 rpm. The relative protein concentration at 44°C is 30 and 6% higher compared to that at 30 and 37°C, respectively.

KW - Agitation speed

KW - Bioreactor

KW - E. coli

KW - HBcAg

KW - Temperature

UR - https://www.scopus.com/pages/publications/33646422757

U2 - 10.1007/BF02931902

DO - 10.1007/BF02931902

M3 - Article

AN - SCOPUS:33646422757

SN - 1226-8372

VL - 11

SP - 164

EP - 167

JO - Biotechnology and Bioprocess Engineering

JF - Biotechnology and Bioprocess Engineering

IS - 2

ER -

Production of hepatitis B core antigen in a stirred tank bioreactor: the influence of temperature and agitation

Abstract

UN SDGs

Keywords

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