Production of hepatitis B core antigen in a stirred tank bioreactor: the influence of temperature and agitation

Beng Ti Tey; Mung Ing Chua; Ghee Sung Chua; Michelle Yeen Tan Ng; Dayang Radiah Awang Biak; Wen Siang Tan; Tau Chuan Ling

doi:10.1007/BF02931902

Production of hepatitis B core antigen in a stirred tank bioreactor: the influence of temperature and agitation

Beng Ti Tey, Mung Ing Chua, Ghee Sung Chua, Michelle Yeen Tan Ng, Dayang Radiah Awang Biak, Wen Siang Tan, Tau Chuan Ling

Research output: Contribution to journal › Article › Research › peer-review

2 Citations (Scopus)

Abstract

The influence of temperature and agitation on the growth of Escherichia coli expressing hepatitis B core antigen (HBcAg) in stirred tank bioreactor were investigated. The highest specific growth rate for E. coli (0.844 h^-1) was achieved at a temperature of 37°C and an agitation speed of 250 rpm. The activation energy for the growth of the E. coli strain W31101Q in the stirred tank bioreactor was estimated to be 11 kcal/mol. The highest protein yield was achieved at a temperature of 44°C and an agitation speed of 250 rpm. The relative protein concentration at 44°C is 30 and 6% higher compared to that at 30 and 37°C, respectively.

Original language	English
Pages (from-to)	164-167
Number of pages	4
Journal	Biotechnology and Bioprocess Engineering
Volume	11
Issue number	2
DOIs	https://doi.org/10.1007/BF02931902
Publication status	Published - Mar 2006
Externally published	Yes

Keywords

Agitation speed
Bioreactor
E. coli
HBcAg
Temperature

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/BF02931902

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title = "Production of hepatitis B core antigen in a stirred tank bioreactor: the influence of temperature and agitation",

abstract = "The influence of temperature and agitation on the growth of Escherichia coli expressing hepatitis B core antigen (HBcAg) in stirred tank bioreactor were investigated. The highest specific growth rate for E. coli (0.844 h-1) was achieved at a temperature of 37°C and an agitation speed of 250 rpm. The activation energy for the growth of the E. coli strain W31101Q in the stirred tank bioreactor was estimated to be 11 kcal/mol. The highest protein yield was achieved at a temperature of 44°C and an agitation speed of 250 rpm. The relative protein concentration at 44°C is 30 and 6% higher compared to that at 30 and 37°C, respectively.",

keywords = "Agitation speed, Bioreactor, E. coli, HBcAg, Temperature",

author = "Tey, {Beng Ti} and Chua, {Mung Ing} and Chua, {Ghee Sung} and {Tan Ng}, {Michelle Yeen} and {Awang Biak}, {Dayang Radiah} and Tan, {Wen Siang} and Ling, {Tau Chuan}",

note = "Funding Information: ^{\AA}{\^a}{\aa}{\c c}{\"i}{\"a}{\'E}{\c C}{\"O}{\'E}{\~a}{\'E}{\aa}{\'i} This study was supported by an IRPA Grant (09-02-04-0766-EA001) from the Ministry of Science, Technology, and Innovation, Malaysia. Copyright: Copyright 2009 Elsevier B.V., All rights reserved.",

year = "2006",

month = mar,

doi = "10.1007/BF02931902",

language = "English",

volume = "11",

pages = "164--167",

journal = "Biotechnology and Bioprocess Engineering",

issn = "1226-8372",

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number = "2",

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TY - JOUR

T1 - Production of hepatitis B core antigen in a stirred tank bioreactor

T2 - the influence of temperature and agitation

AU - Tey, Beng Ti

AU - Chua, Mung Ing

AU - Chua, Ghee Sung

AU - Tan Ng, Michelle Yeen

AU - Awang Biak, Dayang Radiah

AU - Tan, Wen Siang

AU - Ling, Tau Chuan

N1 - Funding Information: ^ÅâåçïäÉÇÖÉãÉåí This study was supported by an IRPA Grant (09-02-04-0766-EA001) from the Ministry of Science, Technology, and Innovation, Malaysia. Copyright: Copyright 2009 Elsevier B.V., All rights reserved.

PY - 2006/3

Y1 - 2006/3

N2 - The influence of temperature and agitation on the growth of Escherichia coli expressing hepatitis B core antigen (HBcAg) in stirred tank bioreactor were investigated. The highest specific growth rate for E. coli (0.844 h-1) was achieved at a temperature of 37°C and an agitation speed of 250 rpm. The activation energy for the growth of the E. coli strain W31101Q in the stirred tank bioreactor was estimated to be 11 kcal/mol. The highest protein yield was achieved at a temperature of 44°C and an agitation speed of 250 rpm. The relative protein concentration at 44°C is 30 and 6% higher compared to that at 30 and 37°C, respectively.

AB - The influence of temperature and agitation on the growth of Escherichia coli expressing hepatitis B core antigen (HBcAg) in stirred tank bioreactor were investigated. The highest specific growth rate for E. coli (0.844 h-1) was achieved at a temperature of 37°C and an agitation speed of 250 rpm. The activation energy for the growth of the E. coli strain W31101Q in the stirred tank bioreactor was estimated to be 11 kcal/mol. The highest protein yield was achieved at a temperature of 44°C and an agitation speed of 250 rpm. The relative protein concentration at 44°C is 30 and 6% higher compared to that at 30 and 37°C, respectively.

KW - Agitation speed

KW - Bioreactor

KW - E. coli

KW - HBcAg

KW - Temperature

UR - http://www.scopus.com/inward/record.url?scp=33646422757&partnerID=8YFLogxK

U2 - 10.1007/BF02931902

DO - 10.1007/BF02931902

M3 - Article

AN - SCOPUS:33646422757

SN - 1226-8372

VL - 11

SP - 164

EP - 167

JO - Biotechnology and Bioprocess Engineering

JF - Biotechnology and Bioprocess Engineering

IS - 2

ER -

Production of hepatitis B core antigen in a stirred tank bioreactor: the influence of temperature and agitation

Abstract

Keywords

UN SDGs

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