Production of biodiesel from palm oil using liquid core lipase encapsulated in κ-carrageenan

Kenthorai Raman Jegannathan, Leong Jun-Yee, Eng Seng Chan, Pogaku Ravindra

Research output: Contribution to journalArticleResearchpeer-review

84 Citations (Scopus)


This work deals with the enzymatic transesterification of palm oil with methanol in a solvent-free system. Among the five lipases tested in the initial screening, lipase PS from Burkholderia cepacia resulted in the highest triglyceride conversion. Lipase PS was further investigated in a novel immobilized form by encapsulating within a biopolymer, κ-carrageenan. Using the immobilized lipase the production parameters of biodiesel from palm oil were optimized. The optimal conditions for processing 10 g of palm oil was: 30 °C, 1:7 oil/methanol molar ratio, 1 g water, 5.25 g immobilized lipase, 72 h reaction time and 23.7g relative centrifugal force. At the optimal conditions, triglyceride conversion of up to 100% could be obtained. The immobilized lipase was stable and retained 82% relative transesterification activity after five cycles. Liquid core lipase encapsulated in κ-carrageenan could be a potential immobilized catalyst for eco-friendly production of biodiesel.

Original languageEnglish
Pages (from-to)2272-2277
Number of pages6
Issue number9
Publication statusPublished - 2010
Externally publishedYes


  • Biodiesel
  • Encapsulation
  • Immobilization
  • Lipase
  • Liquid core

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