Production and faecal fermentation of pentose oligomers of hemicellulose

Study of variables influencing bioprocess efficiency

Ramkrishna D. Singh, Daniel So, C. K. Yao, Paul Gill, Naresh Pillai, Jane Muir, Amit Arora

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Almond shell, a by-product obtained from the nut industry, was valorised into low degree of polymerisation xylooligosaccharides using alkaline pretreatment and enzymatic hydrolysis. The effect of particle size on hemicellulose recovery upon pretreatment was studied using 1 and 2 M NaOH. It was observed that particle size significantly influences hemicellulose recovery, as particles below 120 µm resulted in near complete recovery at 2 M NaOH. Enzymatic hydrolysis of hemicellulose was optimised using response surface methodology, to obtain efficient xylooligosaccharides production at low enzyme dose and high substrate concentration. For higher XOS yield, an enzyme dose of 10 U and substrate concentration <2% was optimal. The in-vitro human faecal fermentation study revealed no significant difference in gas and short chain fatty acid level among substrates evaluated. It was observed that short chain oligosaccharides produce higher level of acetate than medium chain oligosaccharides.

Original languageEnglish
Article number124945
Number of pages7
JournalFood Chemistry
Volume297
DOIs
Publication statusPublished - 1 Nov 2019

Keywords

  • Alkaline extraction
  • Hemicellulose
  • Short chain fatty acids
  • Xylooligosaccharides

Cite this

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title = "Production and faecal fermentation of pentose oligomers of hemicellulose: Study of variables influencing bioprocess efficiency",
abstract = "Almond shell, a by-product obtained from the nut industry, was valorised into low degree of polymerisation xylooligosaccharides using alkaline pretreatment and enzymatic hydrolysis. The effect of particle size on hemicellulose recovery upon pretreatment was studied using 1 and 2 M NaOH. It was observed that particle size significantly influences hemicellulose recovery, as particles below 120 µm resulted in near complete recovery at 2 M NaOH. Enzymatic hydrolysis of hemicellulose was optimised using response surface methodology, to obtain efficient xylooligosaccharides production at low enzyme dose and high substrate concentration. For higher XOS yield, an enzyme dose of 10 U and substrate concentration <2{\%} was optimal. The in-vitro human faecal fermentation study revealed no significant difference in gas and short chain fatty acid level among substrates evaluated. It was observed that short chain oligosaccharides produce higher level of acetate than medium chain oligosaccharides.",
keywords = "Alkaline extraction, Hemicellulose, Short chain fatty acids, Xylooligosaccharides",
author = "Singh, {Ramkrishna D.} and Daniel So and Yao, {C. K.} and Paul Gill and Naresh Pillai and Jane Muir and Amit Arora",
year = "2019",
month = "11",
day = "1",
doi = "10.1016/j.foodchem.2019.06.012",
language = "English",
volume = "297",
journal = "Food Chemistry",
issn = "0308-8146",
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Production and faecal fermentation of pentose oligomers of hemicellulose : Study of variables influencing bioprocess efficiency. / Singh, Ramkrishna D.; So, Daniel; Yao, C. K.; Gill, Paul; Pillai, Naresh; Muir, Jane; Arora, Amit.

In: Food Chemistry, Vol. 297, 124945, 01.11.2019.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Production and faecal fermentation of pentose oligomers of hemicellulose

T2 - Study of variables influencing bioprocess efficiency

AU - Singh, Ramkrishna D.

AU - So, Daniel

AU - Yao, C. K.

AU - Gill, Paul

AU - Pillai, Naresh

AU - Muir, Jane

AU - Arora, Amit

PY - 2019/11/1

Y1 - 2019/11/1

N2 - Almond shell, a by-product obtained from the nut industry, was valorised into low degree of polymerisation xylooligosaccharides using alkaline pretreatment and enzymatic hydrolysis. The effect of particle size on hemicellulose recovery upon pretreatment was studied using 1 and 2 M NaOH. It was observed that particle size significantly influences hemicellulose recovery, as particles below 120 µm resulted in near complete recovery at 2 M NaOH. Enzymatic hydrolysis of hemicellulose was optimised using response surface methodology, to obtain efficient xylooligosaccharides production at low enzyme dose and high substrate concentration. For higher XOS yield, an enzyme dose of 10 U and substrate concentration <2% was optimal. The in-vitro human faecal fermentation study revealed no significant difference in gas and short chain fatty acid level among substrates evaluated. It was observed that short chain oligosaccharides produce higher level of acetate than medium chain oligosaccharides.

AB - Almond shell, a by-product obtained from the nut industry, was valorised into low degree of polymerisation xylooligosaccharides using alkaline pretreatment and enzymatic hydrolysis. The effect of particle size on hemicellulose recovery upon pretreatment was studied using 1 and 2 M NaOH. It was observed that particle size significantly influences hemicellulose recovery, as particles below 120 µm resulted in near complete recovery at 2 M NaOH. Enzymatic hydrolysis of hemicellulose was optimised using response surface methodology, to obtain efficient xylooligosaccharides production at low enzyme dose and high substrate concentration. For higher XOS yield, an enzyme dose of 10 U and substrate concentration <2% was optimal. The in-vitro human faecal fermentation study revealed no significant difference in gas and short chain fatty acid level among substrates evaluated. It was observed that short chain oligosaccharides produce higher level of acetate than medium chain oligosaccharides.

KW - Alkaline extraction

KW - Hemicellulose

KW - Short chain fatty acids

KW - Xylooligosaccharides

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M3 - Article

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