Production and characterization of recombinant human proteinase inhibitor 6 expressed in Pichia pastoris

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Abstract

The human intracellular serine proteinase inhibitor, proteinase inhibitor 6 (PI-6), was expressed in the methylotropic yeast Pichia pastoris. The PI-6 cDNA was modified to encode six histidine residues immediately after the initiation codon, and was placed under the control of the P. pastoris alcohol oxidase promoter in the vector pHIL-D2. On methanol induction, active recombinant PI-6 was produced within the yeast cells, and following cell lysis, was separated from yeast proteins by affinity chromatography using nickel nitrilo-tri-acetic acid (NTA) resin. The interaction of recombinant PI-6 with a range of serine proteinases was studied. Second order association rate constants (ka) were derived for the interaction with trypsin (1.8 · 106 M-1s-1), thrombin (1.2 · 105 M-1 s-1), urokinase plasminogen activator (4.0 · 104 M-1s-1), plasmin (1.3 · 106 M-1s-1), and activated protein C (7.5 · 103 M-1 s-1). By monitoring complex formation, recombinant PI-6 was also shown to interact with factor Xa. No complex formation was observed with chymotrypsin, human leukocyte elastase, cathepsin G and tissue plasminogen activator, although PI-6 is apparently a substrate for chymotrypsin, leukocyte elastase and cathepsin G.

Original languageEnglish
Pages (from-to)28-34
Number of pages7
JournalBiochimica et Biophysica Acta (BBA)/Protein Structure and Molecular
Volume1252
Issue number1
DOIs
Publication statusPublished - 27 Sep 1995
Externally publishedYes

Keywords

  • (P. pastoris)
  • Gene expression
  • Placental thrombin inhibitor
  • Proteinase inhibitor 6
  • Serine proteinase inhibitor
  • Serpin

Cite this

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title = "Production and characterization of recombinant human proteinase inhibitor 6 expressed in Pichia pastoris",
abstract = "The human intracellular serine proteinase inhibitor, proteinase inhibitor 6 (PI-6), was expressed in the methylotropic yeast Pichia pastoris. The PI-6 cDNA was modified to encode six histidine residues immediately after the initiation codon, and was placed under the control of the P. pastoris alcohol oxidase promoter in the vector pHIL-D2. On methanol induction, active recombinant PI-6 was produced within the yeast cells, and following cell lysis, was separated from yeast proteins by affinity chromatography using nickel nitrilo-tri-acetic acid (NTA) resin. The interaction of recombinant PI-6 with a range of serine proteinases was studied. Second order association rate constants (ka) were derived for the interaction with trypsin (1.8 · 106 M-1s-1), thrombin (1.2 · 105 M-1 s-1), urokinase plasminogen activator (4.0 · 104 M-1s-1), plasmin (1.3 · 106 M-1s-1), and activated protein C (7.5 · 103 M-1 s-1). By monitoring complex formation, recombinant PI-6 was also shown to interact with factor Xa. No complex formation was observed with chymotrypsin, human leukocyte elastase, cathepsin G and tissue plasminogen activator, although PI-6 is apparently a substrate for chymotrypsin, leukocyte elastase and cathepsin G.",
keywords = "(P. pastoris), Gene expression, Placental thrombin inhibitor, Proteinase inhibitor 6, Serine proteinase inhibitor, Serpin",
author = "Jiuru Sun and Paul Coughlin and Salem, {Hatem H.} and Phillip Bird",
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AU - Salem, Hatem H.

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N2 - The human intracellular serine proteinase inhibitor, proteinase inhibitor 6 (PI-6), was expressed in the methylotropic yeast Pichia pastoris. The PI-6 cDNA was modified to encode six histidine residues immediately after the initiation codon, and was placed under the control of the P. pastoris alcohol oxidase promoter in the vector pHIL-D2. On methanol induction, active recombinant PI-6 was produced within the yeast cells, and following cell lysis, was separated from yeast proteins by affinity chromatography using nickel nitrilo-tri-acetic acid (NTA) resin. The interaction of recombinant PI-6 with a range of serine proteinases was studied. Second order association rate constants (ka) were derived for the interaction with trypsin (1.8 · 106 M-1s-1), thrombin (1.2 · 105 M-1 s-1), urokinase plasminogen activator (4.0 · 104 M-1s-1), plasmin (1.3 · 106 M-1s-1), and activated protein C (7.5 · 103 M-1 s-1). By monitoring complex formation, recombinant PI-6 was also shown to interact with factor Xa. No complex formation was observed with chymotrypsin, human leukocyte elastase, cathepsin G and tissue plasminogen activator, although PI-6 is apparently a substrate for chymotrypsin, leukocyte elastase and cathepsin G.

AB - The human intracellular serine proteinase inhibitor, proteinase inhibitor 6 (PI-6), was expressed in the methylotropic yeast Pichia pastoris. The PI-6 cDNA was modified to encode six histidine residues immediately after the initiation codon, and was placed under the control of the P. pastoris alcohol oxidase promoter in the vector pHIL-D2. On methanol induction, active recombinant PI-6 was produced within the yeast cells, and following cell lysis, was separated from yeast proteins by affinity chromatography using nickel nitrilo-tri-acetic acid (NTA) resin. The interaction of recombinant PI-6 with a range of serine proteinases was studied. Second order association rate constants (ka) were derived for the interaction with trypsin (1.8 · 106 M-1s-1), thrombin (1.2 · 105 M-1 s-1), urokinase plasminogen activator (4.0 · 104 M-1s-1), plasmin (1.3 · 106 M-1s-1), and activated protein C (7.5 · 103 M-1 s-1). By monitoring complex formation, recombinant PI-6 was also shown to interact with factor Xa. No complex formation was observed with chymotrypsin, human leukocyte elastase, cathepsin G and tissue plasminogen activator, although PI-6 is apparently a substrate for chymotrypsin, leukocyte elastase and cathepsin G.

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