Probes to monitor activity of the paracaspase MALT1

Janna Hachmann, Laura E. Edgington-Mitchell, Marcin Poreba, Laura E. Sanman, Marcin Drag, Matthew Bogyo, Guy S. Salvesen

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14 Citations (Scopus)

Abstract

The human paracaspase mucosa-associated lymphoid tissue lymphoma translocation protein 1 (MALT1) plays a central role in nuclear factor-κB (NF-κB) signaling as both a protease and scaffolding protein. Knocking out MALT1 leads to impaired NF-κB signaling and failure to mount an effective immune response. However, it is unclear to which degree it is the scaffolding function versus the proteolytic activity of MALT1 that is essential. Previous work involving a MALT1 inhibitor with low selectivity suggests that the enzymatic function plays an important role in different cell lines. To help elucidate this proteolytic role of MALT1, we have designed activity-based probes that inhibit its proteolytic activity. The probes selectively label active enzyme and can be used to inhibit MALT1 and trace its activity profile, helping to create a better picture of the significance of the proteolytic function of MALT1.

Original languageEnglish
Pages (from-to)139-147
Number of pages9
JournalChemistry and Biology
Volume22
Issue number1
DOIs
Publication statusPublished - 22 Jan 2015
Externally publishedYes

Cite this

Hachmann, J., Edgington-Mitchell, L. E., Poreba, M., Sanman, L. E., Drag, M., Bogyo, M., & Salvesen, G. S. (2015). Probes to monitor activity of the paracaspase MALT1. Chemistry and Biology, 22(1), 139-147. https://doi.org/10.1016/j.chembiol.2014.11.011