TY - JOUR
T1 - Probes to monitor activity of the paracaspase MALT1
AU - Hachmann, Janna
AU - Edgington-Mitchell, Laura E.
AU - Poreba, Marcin
AU - Sanman, Laura E.
AU - Drag, Marcin
AU - Bogyo, Matthew
AU - Salvesen, Guy S.
PY - 2015/1/22
Y1 - 2015/1/22
N2 - The human paracaspase mucosa-associated lymphoid tissue lymphoma translocation protein 1 (MALT1) plays a central role in nuclear factor-κB (NF-κB) signaling as both a protease and scaffolding protein. Knocking out MALT1 leads to impaired NF-κB signaling and failure to mount an effective immune response. However, it is unclear to which degree it is the scaffolding function versus the proteolytic activity of MALT1 that is essential. Previous work involving a MALT1 inhibitor with low selectivity suggests that the enzymatic function plays an important role in different cell lines. To help elucidate this proteolytic role of MALT1, we have designed activity-based probes that inhibit its proteolytic activity. The probes selectively label active enzyme and can be used to inhibit MALT1 and trace its activity profile, helping to create a better picture of the significance of the proteolytic function of MALT1.
AB - The human paracaspase mucosa-associated lymphoid tissue lymphoma translocation protein 1 (MALT1) plays a central role in nuclear factor-κB (NF-κB) signaling as both a protease and scaffolding protein. Knocking out MALT1 leads to impaired NF-κB signaling and failure to mount an effective immune response. However, it is unclear to which degree it is the scaffolding function versus the proteolytic activity of MALT1 that is essential. Previous work involving a MALT1 inhibitor with low selectivity suggests that the enzymatic function plays an important role in different cell lines. To help elucidate this proteolytic role of MALT1, we have designed activity-based probes that inhibit its proteolytic activity. The probes selectively label active enzyme and can be used to inhibit MALT1 and trace its activity profile, helping to create a better picture of the significance of the proteolytic function of MALT1.
UR - http://www.scopus.com/inward/record.url?scp=84921527332&partnerID=8YFLogxK
U2 - 10.1016/j.chembiol.2014.11.011
DO - 10.1016/j.chembiol.2014.11.011
M3 - Article
C2 - 25556944
AN - SCOPUS:84921527332
SN - 1074-5521
VL - 22
SP - 139
EP - 147
JO - Chemistry and Biology
JF - Chemistry and Biology
IS - 1
ER -