Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 268 - 275 |
| Number of pages | 8 |
| Journal | Sensors and Actuators B: Chemical |
| Volume | 219 |
| DOIs | |
| Publication status | Published - 2015 |
Cite this
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Printed two-dimensional micro-ring film plate for spot assays and its functionalization by immobilized enzymes. / Zhao, Zhengyang; Shen, Wei; He, Lizhong; Tian, Junfei.
In: Sensors and Actuators B: Chemical, Vol. 219, 2015, p. 268 - 275.Research output: Contribution to journal › Article › Research › peer-review
TY - JOUR
T1 - Printed two-dimensional micro-ring film plate for spot assays and its functionalization by immobilized enzymes
AU - Zhao, Zhengyang
AU - Shen, Wei
AU - He, Lizhong
AU - Tian, Junfei
PY - 2015
Y1 - 2015
N2 - In this paper, we present a simple printable two-dimensional micro-ring plate for spot assays in low-resource settings. Each micro-zone on the overhead transparency contains a hydrophilic center restricted by a superhydrophobic ring. The zone can be fully wetted by a small amount of aqueous sample (0.5 mu l) and can also hold a large volume up to 100 mu l, offering the flexibility to handle different amount of samples. Bio-analysis capacity of the micro-ring plate was demonstrated by the Pierce 660 nm protein assay and blood typing assay. We further functionalized the plate by depositing enzymes-immobilized nanorods into micro-zones. This functionalization, for the first time, enables multi-diagnostic functions in one micro-ring as demonstrated by analysis of individual substrate of the two enzymes. The functionalized plates show a high stability under varied temperatures that mimic the changes of environment during transport. Importantly, all the assays were carried out using non-laboratory devices such as mobile phone, ensuring its low-cost application under limited resources.
AB - In this paper, we present a simple printable two-dimensional micro-ring plate for spot assays in low-resource settings. Each micro-zone on the overhead transparency contains a hydrophilic center restricted by a superhydrophobic ring. The zone can be fully wetted by a small amount of aqueous sample (0.5 mu l) and can also hold a large volume up to 100 mu l, offering the flexibility to handle different amount of samples. Bio-analysis capacity of the micro-ring plate was demonstrated by the Pierce 660 nm protein assay and blood typing assay. We further functionalized the plate by depositing enzymes-immobilized nanorods into micro-zones. This functionalization, for the first time, enables multi-diagnostic functions in one micro-ring as demonstrated by analysis of individual substrate of the two enzymes. The functionalized plates show a high stability under varied temperatures that mimic the changes of environment during transport. Importantly, all the assays were carried out using non-laboratory devices such as mobile phone, ensuring its low-cost application under limited resources.
UR - http://goo.gl/wYEENJ
U2 - 10.1016/j.snb.2015.05.033
DO - 10.1016/j.snb.2015.05.033
M3 - Article
VL - 219
SP - 268
EP - 275
JO - Sensors and Actuators B: Chemical
JF - Sensors and Actuators B: Chemical
SN - 0925-4005
ER -