Printed two-dimensional micro-ring film plate for spot assays and its functionalization by immobilized enzymes

Zhengyang Zhao, Wei Shen, Lizhong He, Junfei Tian

Research output: Contribution to journalArticleResearchpeer-review

4 Citations (Scopus)

Abstract

In this paper, we present a simple printable two-dimensional micro-ring plate for spot assays in low-resource settings. Each micro-zone on the overhead transparency contains a hydrophilic center restricted by a superhydrophobic ring. The zone can be fully wetted by a small amount of aqueous sample (0.5 mu l) and can also hold a large volume up to 100 mu l, offering the flexibility to handle different amount of samples. Bio-analysis capacity of the micro-ring plate was demonstrated by the Pierce 660 nm protein assay and blood typing assay. We further functionalized the plate by depositing enzymes-immobilized nanorods into micro-zones. This functionalization, for the first time, enables multi-diagnostic functions in one micro-ring as demonstrated by analysis of individual substrate of the two enzymes. The functionalized plates show a high stability under varied temperatures that mimic the changes of environment during transport. Importantly, all the assays were carried out using non-laboratory devices such as mobile phone, ensuring its low-cost application under limited resources.
Original languageEnglish
Pages (from-to)268 - 275
Number of pages8
JournalSensors and Actuators B: Chemical
Volume219
DOIs
Publication statusPublished - 2015

Cite this

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title = "Printed two-dimensional micro-ring film plate for spot assays and its functionalization by immobilized enzymes",
abstract = "In this paper, we present a simple printable two-dimensional micro-ring plate for spot assays in low-resource settings. Each micro-zone on the overhead transparency contains a hydrophilic center restricted by a superhydrophobic ring. The zone can be fully wetted by a small amount of aqueous sample (0.5 mu l) and can also hold a large volume up to 100 mu l, offering the flexibility to handle different amount of samples. Bio-analysis capacity of the micro-ring plate was demonstrated by the Pierce 660 nm protein assay and blood typing assay. We further functionalized the plate by depositing enzymes-immobilized nanorods into micro-zones. This functionalization, for the first time, enables multi-diagnostic functions in one micro-ring as demonstrated by analysis of individual substrate of the two enzymes. The functionalized plates show a high stability under varied temperatures that mimic the changes of environment during transport. Importantly, all the assays were carried out using non-laboratory devices such as mobile phone, ensuring its low-cost application under limited resources.",
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Printed two-dimensional micro-ring film plate for spot assays and its functionalization by immobilized enzymes. / Zhao, Zhengyang; Shen, Wei; He, Lizhong; Tian, Junfei.

In: Sensors and Actuators B: Chemical, Vol. 219, 2015, p. 268 - 275.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

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AU - Shen, Wei

AU - He, Lizhong

AU - Tian, Junfei

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AB - In this paper, we present a simple printable two-dimensional micro-ring plate for spot assays in low-resource settings. Each micro-zone on the overhead transparency contains a hydrophilic center restricted by a superhydrophobic ring. The zone can be fully wetted by a small amount of aqueous sample (0.5 mu l) and can also hold a large volume up to 100 mu l, offering the flexibility to handle different amount of samples. Bio-analysis capacity of the micro-ring plate was demonstrated by the Pierce 660 nm protein assay and blood typing assay. We further functionalized the plate by depositing enzymes-immobilized nanorods into micro-zones. This functionalization, for the first time, enables multi-diagnostic functions in one micro-ring as demonstrated by analysis of individual substrate of the two enzymes. The functionalized plates show a high stability under varied temperatures that mimic the changes of environment during transport. Importantly, all the assays were carried out using non-laboratory devices such as mobile phone, ensuring its low-cost application under limited resources.

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