Primary structure of ovine pituitary basic fibroblast growth factor

Richard J. Simpson, Robert L. Moritz, Christopher J. Lloyd, Louis J. Fabri, Edouard C. Nice, Michael R. Rubira, Antony W. Burgess

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16 Citations (Scopus)

Abstract

The complete amino acid sequence of basic FGF (146 residues) from ovine pituitary glands has been established. This has been achieved by the sequence analysis of subnanomole amounts of the intact molecule and of peptides derived by enzymatic digestions with clostripain, chymotrypsin, pepsin and Staphylococcus aureus V8 protease. Microbore HPLC, employing 1-2 mm i.d. columns, was used to purify, concentrate and buffer-exchange the FGF peptides. A novel application of ion-pairing chromatography was employed to isolate peptides which were not retained on conventional reversed-phase systems. There is only one positional difference between the ovine and bovine basic FGFs, but there are 3 positional differences between ovine and human basic FGFS.

Original languageEnglish
Pages (from-to)128-132
Number of pages5
JournalFEBS Letters
Volume224
Issue number1
DOIs
Publication statusPublished - 16 Nov 1987
Externally publishedYes

Keywords

  • Fibroblast growth factor
  • Ion-pair chromatography
  • Microbore HPLC
  • Microsequencing
  • Primary structure

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