Prevalence and associated risk factors of Giardia duodenalis infection among school-going children in Nepal

Sarmila Tandukar; Jeevan B. Sherchand; Jia Xue; Sital Uprety; Samendra P. Sherchan; Dinesh Bhandari; Bikash Malla; Rajani Ghaju Shrestha; Laxmi Parajuli; Shristi Poudel; Ashmita Dhital; Eiji Haramoto

doi:10.1007/s00436-017-5706-5

Prevalence and associated risk factors of Giardia duodenalis infection among school-going children in Nepal

Sarmila Tandukar, Jeevan B. Sherchand, Jia Xue, Sital Uprety, Samendra P. Sherchan, Dinesh Bhandari, Bikash Malla, Rajani Ghaju Shrestha, Laxmi Parajuli, Shristi Poudel, Ashmita Dhital, Eiji Haramoto

Research output: Contribution to journal › Article › Research › peer-review

13 Citations (Scopus)

Abstract

This study aimed to determine the prevalence of intestinal parasites and its associated risk factors among school-going children in Kathmandu, Nepal. Between August and September 2016, a total of 333 stool samples were collected from children at five public schools. The collected samples were subjected to formol-ether concentration, followed by conventional microscopic examination for intestinal parasites. The overall prevalence of intestinal parasites was 24.3% (81/333), with Giardia spp. showing the highest prevalence of 18.9% (63/333). Samples positive for Giardia spp. by microscopy were further subjected to quantitative polymerase chain reaction (qPCR) for G. duodenalis, resulting in a positive ratio of 100%. The positive ratio of Giardia spp. was considerably high among children consuming tanker water (27.3%), jar water (21.0%), and tap water (17.5%). Our results demonstrated that G. duodenalis remains predominant in school-going children in Nepal.

Original language	English
Pages (from-to)	287-293
Number of pages	7
Journal	Parasitology Research
Volume	117
Issue number	1
DOIs	https://doi.org/10.1007/s00436-017-5706-5
Publication status	Published - Jan 2018
Externally published	Yes

Keywords

Giardia duodenalis
Nepal
Quantitative polymerase chain reaction
School-going children
Stool

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/s00436-017-5706-5

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title = "Prevalence and associated risk factors of Giardia duodenalis infection among school-going children in Nepal",

abstract = "This study aimed to determine the prevalence of intestinal parasites and its associated risk factors among school-going children in Kathmandu, Nepal. Between August and September 2016, a total of 333 stool samples were collected from children at five public schools. The collected samples were subjected to formol-ether concentration, followed by conventional microscopic examination for intestinal parasites. The overall prevalence of intestinal parasites was 24.3\% (81/333), with Giardia spp. showing the highest prevalence of 18.9\% (63/333). Samples positive for Giardia spp. by microscopy were further subjected to quantitative polymerase chain reaction (qPCR) for G. duodenalis, resulting in a positive ratio of 100\%. The positive ratio of Giardia spp. was considerably high among children consuming tanker water (27.3\%), jar water (21.0\%), and tap water (17.5\%). Our results demonstrated that G. duodenalis remains predominant in school-going children in Nepal.",

keywords = "Giardia duodenalis, Nepal, Quantitative polymerase chain reaction, School-going children, Stool",

author = "Sarmila Tandukar and Sherchand, \{Jeevan B.\} and Jia Xue and Sital Uprety and Sherchan, \{Samendra P.\} and Dinesh Bhandari and Bikash Malla and \{Ghaju Shrestha\}, Rajani and Laxmi Parajuli and Shristi Poudel and Ashmita Dhital and Eiji Haramoto",

note = "Publisher Copyright: {\textcopyright} 2017, Springer-Verlag GmbH Germany, part of Springer Nature.",

year = "2018",

month = jan,

doi = "10.1007/s00436-017-5706-5",

language = "English",

volume = "117",

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journal = "Parasitology Research",

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TY - JOUR

T1 - Prevalence and associated risk factors of Giardia duodenalis infection among school-going children in Nepal

AU - Tandukar, Sarmila

AU - Sherchand, Jeevan B.

AU - Xue, Jia

AU - Uprety, Sital

AU - Sherchan, Samendra P.

AU - Bhandari, Dinesh

AU - Malla, Bikash

AU - Ghaju Shrestha, Rajani

AU - Parajuli, Laxmi

AU - Poudel, Shristi

AU - Dhital, Ashmita

AU - Haramoto, Eiji

PY - 2018/1

Y1 - 2018/1

N2 - This study aimed to determine the prevalence of intestinal parasites and its associated risk factors among school-going children in Kathmandu, Nepal. Between August and September 2016, a total of 333 stool samples were collected from children at five public schools. The collected samples were subjected to formol-ether concentration, followed by conventional microscopic examination for intestinal parasites. The overall prevalence of intestinal parasites was 24.3% (81/333), with Giardia spp. showing the highest prevalence of 18.9% (63/333). Samples positive for Giardia spp. by microscopy were further subjected to quantitative polymerase chain reaction (qPCR) for G. duodenalis, resulting in a positive ratio of 100%. The positive ratio of Giardia spp. was considerably high among children consuming tanker water (27.3%), jar water (21.0%), and tap water (17.5%). Our results demonstrated that G. duodenalis remains predominant in school-going children in Nepal.

AB - This study aimed to determine the prevalence of intestinal parasites and its associated risk factors among school-going children in Kathmandu, Nepal. Between August and September 2016, a total of 333 stool samples were collected from children at five public schools. The collected samples were subjected to formol-ether concentration, followed by conventional microscopic examination for intestinal parasites. The overall prevalence of intestinal parasites was 24.3% (81/333), with Giardia spp. showing the highest prevalence of 18.9% (63/333). Samples positive for Giardia spp. by microscopy were further subjected to quantitative polymerase chain reaction (qPCR) for G. duodenalis, resulting in a positive ratio of 100%. The positive ratio of Giardia spp. was considerably high among children consuming tanker water (27.3%), jar water (21.0%), and tap water (17.5%). Our results demonstrated that G. duodenalis remains predominant in school-going children in Nepal.

KW - Giardia duodenalis

KW - Nepal

KW - Quantitative polymerase chain reaction

KW - School-going children

KW - Stool

UR - https://www.scopus.com/pages/publications/85037372959

U2 - 10.1007/s00436-017-5706-5

DO - 10.1007/s00436-017-5706-5

M3 - Article

C2 - 29218443

AN - SCOPUS:85037372959

SN - 0932-0113

VL - 117

SP - 287

EP - 293

JO - Parasitology Research

JF - Parasitology Research

IS - 1

ER -

Prevalence and associated risk factors of Giardia duodenalis infection among school-going children in Nepal

Abstract

Keywords

UN SDGs

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