Presynaptic adenosine A2a receptors on soma and central terminals of rat vagal afferent neurons

Margie Castillo-Meléndez, Elena Krstew, Andrew J. Lawrence, Bevyn Jarrott

Research output: Contribution to journalArticleResearchpeer-review

60 Citations (Scopus)

Abstract

The dorsal vagal complex of the medulla oblongata is a key centre involved in the regulation of numerous autonomic functions, including cardiovascular control. Adenosine has been implicated as a potential neuromodulator of the baroreceptor reflex, and therefore the current study has investigated the presence and characteristics of adenosine receptors on rat vagal afferent neurons. In the nodose-vagal grease gap preparation, the adenosine A2a agonist CGS-21680 evoked a depolarisation only in the presence of the selective adenosine A1 antagonist PACPX. Autoradiography using [3H]NECA (4 nM) with suppression of A1 binding enabled the first visualisation of high affinity adenosine A2 receptors in the nucleus tractus solitarius (NTS). Unilateral nodose ganglionectomy resulted in over 90% reduction in binding in the lesioned (ipsilateral) NTS compared to a sham control. Furthermore, local administration of CGS-21680 increased evoked glutamate release in the NTS, as measured by in vivo microdialysis. These data suggest the presence of presynaptic adenosine A2a receptors on both the soma and central terminals of rat vagal afferent neurons, and thereby support the hypothesis that adenosine may have a modulatory role in the baroreceptor reflex.

Original languageEnglish
Pages (from-to)137-144
Number of pages8
JournalBrain Research
Volume652
Issue number1
DOIs
Publication statusPublished - 25 Jul 1994

Keywords

  • Adenosine
  • Autoradiography
  • Glutamate
  • Microdialysis
  • Nodose ganglion
  • Nucleus tractus solitarius
  • Receptor
  • Vagal afferent

Cite this

Castillo-Meléndez, Margie ; Krstew, Elena ; Lawrence, Andrew J. ; Jarrott, Bevyn. / Presynaptic adenosine A2a receptors on soma and central terminals of rat vagal afferent neurons. In: Brain Research. 1994 ; Vol. 652, No. 1. pp. 137-144.
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Presynaptic adenosine A2a receptors on soma and central terminals of rat vagal afferent neurons. / Castillo-Meléndez, Margie; Krstew, Elena; Lawrence, Andrew J.; Jarrott, Bevyn.

In: Brain Research, Vol. 652, No. 1, 25.07.1994, p. 137-144.

Research output: Contribution to journalArticleResearchpeer-review

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