Preparation of platelet concentrates for research and transfusion purposes

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Otherpeer-review

4 Citations (Scopus)

Abstract

Platelets are specialized cellular elements of the blood that play central roles in physiologic and pathologic processes of hemostasis, wound healing, host defense, thrombosis, inflammation, and tumor metastasis. Activation of platelets is crucial for platelet function that includes a complex interplay of adhesion, signaling molecules, and release of bioactive factors. Transfusion of platelet concentrates is an important treatment component for thrombocytopenia and bleeding. Recent progress in high-throughput mRNA and protein profiling techniques has advanced the understanding of platelet biological functions toward identifying novel platelet-expressed and secreted proteins, analyzing functional changes between normal and pathologic states, and determining the effects of processing and storage on platelet concentrates for transfusion. It is important to understand the different standard methods of platelet preparation and how they differ from the perspective for use as research samples in clinical chemistry. Two simple methods are described here for the preparation of research-scale platelet samples from whole blood, and detailed notes are provided about the methods used for the preparation of platelet concentrates for transfusion.

Original languageEnglish
Title of host publicationSerum/Plasma Proteomics
Subtitle of host publicationMethods and Protocols
PublisherHumana Press
Pages31-42
Number of pages12
Volume1619
Edition2nd
DOIs
Publication statusPublished - 2017

Publication series

NameMethods in Molecular Biology
Volume1619
ISSN (Print)10643745

Keywords

  • Apheresis
  • Buffy coat
  • Platelet concentrate
  • Platelet rich plasma
  • Proteomics
  • Protocol
  • Research
  • SOP
  • Transfusion

Cite this

Greening, D. W., Simpson, R. J., & Sparrow, R. L. (2017). Preparation of platelet concentrates for research and transfusion purposes. In Serum/Plasma Proteomics: Methods and Protocols (2nd ed., Vol. 1619, pp. 31-42). (Methods in Molecular Biology; Vol. 1619). Humana Press. https://doi.org/10.1007/978-1-4939-7057-5_3
Greening, David W ; Simpson, Richard J. ; Sparrow, Rosemary L. / Preparation of platelet concentrates for research and transfusion purposes. Serum/Plasma Proteomics: Methods and Protocols. Vol. 1619 2nd. ed. Humana Press, 2017. pp. 31-42 (Methods in Molecular Biology).
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Greening, DW, Simpson, RJ & Sparrow, RL 2017, Preparation of platelet concentrates for research and transfusion purposes. in Serum/Plasma Proteomics: Methods and Protocols. 2nd edn, vol. 1619, Methods in Molecular Biology, vol. 1619, Humana Press, pp. 31-42. https://doi.org/10.1007/978-1-4939-7057-5_3

Preparation of platelet concentrates for research and transfusion purposes. / Greening, David W; Simpson, Richard J.; Sparrow, Rosemary L.

Serum/Plasma Proteomics: Methods and Protocols. Vol. 1619 2nd. ed. Humana Press, 2017. p. 31-42 (Methods in Molecular Biology; Vol. 1619).

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Otherpeer-review

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Greening DW, Simpson RJ, Sparrow RL. Preparation of platelet concentrates for research and transfusion purposes. In Serum/Plasma Proteomics: Methods and Protocols. 2nd ed. Vol. 1619. Humana Press. 2017. p. 31-42. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-7057-5_3