Abstract
Platelets are specialized cellular elements of the blood that play central roles in physiologic and pathologic processes of hemostasis, wound healing, host defense, thrombosis, inflammation, and tumor metastasis. Activation of platelets is crucial for platelet function that includes a complex interplay of adhesion, signaling molecules, and release of bioactive factors. Transfusion of platelet concentrates is an important treatment component for thrombocytopenia and bleeding. Recent progress in high-throughput mRNA and protein profiling techniques has advanced the understanding of platelet biological functions toward identifying novel platelet-expressed and secreted proteins, analyzing functional changes between normal and pathologic states, and determining the effects of processing and storage on platelet concentrates for transfusion. It is important to understand the different standard methods of platelet preparation and how they differ from the perspective for use as research samples in clinical chemistry. Two simple methods are described here for the preparation of research-scale platelet samples from whole blood, and detailed notes are provided about the methods used for the preparation of platelet concentrates for transfusion.
| Original language | English |
|---|---|
| Title of host publication | Serum/Plasma Proteomics |
| Subtitle of host publication | Methods and Protocols |
| Publisher | Humana Press |
| Pages | 31-42 |
| Number of pages | 12 |
| Volume | 1619 |
| Edition | 2nd |
| DOIs | |
| Publication status | Published - 2017 |
Publication series
| Name | Methods in Molecular Biology |
|---|---|
| Volume | 1619 |
| ISSN (Print) | 10643745 |
Keywords
- Apheresis
- Buffy coat
- Platelet concentrate
- Platelet rich plasma
- Proteomics
- Protocol
- Research
- SOP
- Transfusion
Cite this
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Preparation of platelet concentrates for research and transfusion purposes. / Greening, David W; Simpson, Richard J.; Sparrow, Rosemary L.
Serum/Plasma Proteomics: Methods and Protocols. Vol. 1619 2nd. ed. Humana Press, 2017. p. 31-42 (Methods in Molecular Biology; Vol. 1619).Research output: Chapter in Book/Report/Conference proceeding › Chapter (Book) › Other › peer-review
TY - CHAP
T1 - Preparation of platelet concentrates for research and transfusion purposes
AU - Greening, David W
AU - Simpson, Richard J.
AU - Sparrow, Rosemary L.
PY - 2017
Y1 - 2017
N2 - Platelets are specialized cellular elements of the blood that play central roles in physiologic and pathologic processes of hemostasis, wound healing, host defense, thrombosis, inflammation, and tumor metastasis. Activation of platelets is crucial for platelet function that includes a complex interplay of adhesion, signaling molecules, and release of bioactive factors. Transfusion of platelet concentrates is an important treatment component for thrombocytopenia and bleeding. Recent progress in high-throughput mRNA and protein profiling techniques has advanced the understanding of platelet biological functions toward identifying novel platelet-expressed and secreted proteins, analyzing functional changes between normal and pathologic states, and determining the effects of processing and storage on platelet concentrates for transfusion. It is important to understand the different standard methods of platelet preparation and how they differ from the perspective for use as research samples in clinical chemistry. Two simple methods are described here for the preparation of research-scale platelet samples from whole blood, and detailed notes are provided about the methods used for the preparation of platelet concentrates for transfusion.
AB - Platelets are specialized cellular elements of the blood that play central roles in physiologic and pathologic processes of hemostasis, wound healing, host defense, thrombosis, inflammation, and tumor metastasis. Activation of platelets is crucial for platelet function that includes a complex interplay of adhesion, signaling molecules, and release of bioactive factors. Transfusion of platelet concentrates is an important treatment component for thrombocytopenia and bleeding. Recent progress in high-throughput mRNA and protein profiling techniques has advanced the understanding of platelet biological functions toward identifying novel platelet-expressed and secreted proteins, analyzing functional changes between normal and pathologic states, and determining the effects of processing and storage on platelet concentrates for transfusion. It is important to understand the different standard methods of platelet preparation and how they differ from the perspective for use as research samples in clinical chemistry. Two simple methods are described here for the preparation of research-scale platelet samples from whole blood, and detailed notes are provided about the methods used for the preparation of platelet concentrates for transfusion.
KW - Apheresis
KW - Buffy coat
KW - Platelet concentrate
KW - Platelet rich plasma
KW - Proteomics
KW - Protocol
KW - Research
KW - SOP
KW - Transfusion
UR - http://www.scopus.com/inward/record.url?scp=85021735593&partnerID=8YFLogxK
U2 - 10.1007/978-1-4939-7057-5_3
DO - 10.1007/978-1-4939-7057-5_3
M3 - Chapter (Book)
VL - 1619
T3 - Methods in Molecular Biology
SP - 31
EP - 42
BT - Serum/Plasma Proteomics
PB - Humana Press
ER -