Preparation of platelet concentrates

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Researchpeer-review

Abstract

Platelets are specialized blood cells that play central roles in physiologic and pathologic processes of hemostasis, wound healing, host defense, thrombosis, inflammation, and tumor metastasis. Activation of platelets is crucial for platelet function that includes a complex interplay of adhesion, signaling molecules, and release of bioactive factors. Transfusion of platelet concentrates is an important treatment component for thrombocytopenia and bleeding. Recent progress in high-throughput mRNA and protein profiling techniques has advanced the understanding of platelet biological functions toward identifying novel platelet-expressed and secreted proteins, analyzing functional changes between normal and pathologic states, and determining the effects of processing and storage on platelet concentrates for transfusion. It is important to understand the different standard methods of platelet preparation and how they differ from the perspective for use as research samples in clinical chemistry. Two simple methods are described here for the preparation of research-scale platelet samples from whole blood, and detailed notes are provided about the methods used for the preparation of platelet concentrates for transfusion.
Original languageEnglish
Title of host publicationSerum/Plasma Proteomics
Subtitle of host publicationMethods and Protocols
EditorsRichard J. Simpson, David W. Greening
Place of PublicationNew York NY USA
PublisherHumana Press
Pages267-278
Number of pages12
Volume728
ISBN (Print)9781617790676
DOIs
Publication statusPublished - 2011
Externally publishedYes

Publication series

NameMethods in Molecular Biology
PublisherHumana Press
Volume728
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Cite this

Greening, D. W., Sparrow, R., & Simpson, R. J. (2011). Preparation of platelet concentrates. In R. J. Simpson, & D. W. Greening (Eds.), Serum/Plasma Proteomics: Methods and Protocols (Vol. 728, pp. 267-278). (Methods in Molecular Biology; Vol. 728). New York NY USA: Humana Press. https://doi.org/10.1007/978-1-61779-068-3_18
Greening, David W ; Sparrow, Rosemary ; Simpson, Richard J. / Preparation of platelet concentrates. Serum/Plasma Proteomics: Methods and Protocols. editor / Richard J. Simpson ; David W. Greening. Vol. 728 New York NY USA : Humana Press, 2011. pp. 267-278 (Methods in Molecular Biology).
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Greening, DW, Sparrow, R & Simpson, RJ 2011, Preparation of platelet concentrates. in RJ Simpson & DW Greening (eds), Serum/Plasma Proteomics: Methods and Protocols. vol. 728, Methods in Molecular Biology, vol. 728, Humana Press, New York NY USA, pp. 267-278. https://doi.org/10.1007/978-1-61779-068-3_18

Preparation of platelet concentrates. / Greening, David W; Sparrow, Rosemary; Simpson, Richard J.

Serum/Plasma Proteomics: Methods and Protocols. ed. / Richard J. Simpson; David W. Greening. Vol. 728 New York NY USA : Humana Press, 2011. p. 267-278 (Methods in Molecular Biology; Vol. 728).

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Researchpeer-review

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AB - Platelets are specialized blood cells that play central roles in physiologic and pathologic processes of hemostasis, wound healing, host defense, thrombosis, inflammation, and tumor metastasis. Activation of platelets is crucial for platelet function that includes a complex interplay of adhesion, signaling molecules, and release of bioactive factors. Transfusion of platelet concentrates is an important treatment component for thrombocytopenia and bleeding. Recent progress in high-throughput mRNA and protein profiling techniques has advanced the understanding of platelet biological functions toward identifying novel platelet-expressed and secreted proteins, analyzing functional changes between normal and pathologic states, and determining the effects of processing and storage on platelet concentrates for transfusion. It is important to understand the different standard methods of platelet preparation and how they differ from the perspective for use as research samples in clinical chemistry. Two simple methods are described here for the preparation of research-scale platelet samples from whole blood, and detailed notes are provided about the methods used for the preparation of platelet concentrates for transfusion.

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Greening DW, Sparrow R, Simpson RJ. Preparation of platelet concentrates. In Simpson RJ, Greening DW, editors, Serum/Plasma Proteomics: Methods and Protocols. Vol. 728. New York NY USA: Humana Press. 2011. p. 267-278. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-61779-068-3_18