Preparation of ion exchange mesoporous sieve DEAE-SBA-15 and its application to separation and purification of Der f 2 allergen in dermatophagoides farinae

Jie Lei, Lu Yan Zhang, Sheng Song Ding, Shi Yi Jiang, Cheng Zhong Yu, Dong Yuan Zhao

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2 Citations (Scopus)

Abstract

Mesoporous silica SBA-15 was functionalized by 2-diethylamino-ethanol (DEAE) through the epoxide ring opening of 3-glycidyloxy-propyl-trimethoxy silane grafted on the silica surface (denoted as DEAE-SBA-15). Subsequently, DEAE-SBA-15 was used as the weak anion exchange matrix for chromatography to purify Der f 2 allergen from the spent culture medium extract of Dermatophagoides farinae, one of House dust mites that can cause heavy atopic diseases such as asthma and dermatitis. The structure of DEAE-SBA-15 was confirmed by 13C and 29Si CP MAS NMR and other methods. By SDS-PAGE, Der f 2 was estimated to have a molecular weight of 14000 and appeared as the only separated protein band. The assay results of skin prick test showed that the allergic activity of Der f 2 was retained. The above results indicate that DEAE-SBA-15 materials are promising fixed-matrix candidates in liquid chromatography to purify proteins.

Original languageEnglish
Pages (from-to)793-796
Number of pages4
JournalChemical Journal of Chinese Universities
Volume26
Issue number5
Publication statusPublished - 1 May 2005
Externally publishedYes

Keywords

  • DEAE-SBA-15
  • Der f 2 allergen
  • Dermatophagoides farinae
  • Purification
  • Stationary phase preparation

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