Preparation of Inert Polystyrene Latex Particles as MicroRNA Delivery Vectors by Surfactant-Free RAFT Emulsion Polymerization

Cheuk Ka Poon, Owen Tang, Xin Ming Chen, Binh T T Pham, Guillaume Gody, Carol A. Pollock, Brian S. Hawkett, Sebastien Perrier

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20 Citations (Scopus)

Abstract

We present the preparation of 11 nm polyacrylamide-stabilized polystyrene latex particles for conjugation to a microRNA model by surfactant-free RAFT emulsion polymerization. Our synthetic strategy involved the preparation of amphiphilic polyacrylamide-block-polystyrene copolymers, which were able to self-assemble into polymeric micelles and "grow" into polystyrene latex particles. The surface of these sterically stabilized particles was postmodified with a disulfide-bearing linker for the attachment of the microRNA model, which can be released from the latex particles under reducing conditions. These nanoparticles offer the advantage of ease of preparation via a scaleable process, and the versatility of their synthesis makes them adaptable to a range of applications.

Original languageEnglish
Pages (from-to)965-973
Number of pages9
JournalBiomacromolecules
Volume17
Issue number3
DOIs
Publication statusPublished - 14 Mar 2016

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