Preparation of DNA from embryonic stem cells or other cultured cells

Richard Behringer, Marina Gertsenstein, Kristina Vintersten Nagy, Andras Nagy

Research output: Contribution to journalArticleOtherpeer-review


Characterization of embryonic stem (ES) cell-mediated genome alterations, including random insertional transgenesis, gene trapping, gene targeting, and site-specific recombinase-mediated changes, is performed mostly at the ES cell level, before the introduction of these alterations into a mouse. A detailed characterization requires a larger amount of DNA than is required for the initial detection of the candidates for the desired alteration. This protocol describes the preparation of DNA from a 10-cm tissue culture plate. The cells are trypsinized and lysed, and DNA is recovered from the lysate by organic extraction followed by ethanol precipitation.

Original languageEnglish
Pages (from-to)1016-1018
Number of pages3
JournalCold Spring Harbor Protocols
Issue number12
Publication statusPublished - 1 Dec 2017

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