Preparation of DNA from embryonic stem cells or other cultured cells

Richard Behringer, Marina Gertsenstein, Kristina Vintersten Nagy, Andras Nagy

Research output: Contribution to journalArticleOtherpeer-review

Abstract

Characterization of embryonic stem (ES) cell-mediated genome alterations, including random insertional transgenesis, gene trapping, gene targeting, and site-specific recombinase-mediated changes, is performed mostly at the ES cell level, before the introduction of these alterations into a mouse. A detailed characterization requires a larger amount of DNA than is required for the initial detection of the candidates for the desired alteration. This protocol describes the preparation of DNA from a 10-cm tissue culture plate. The cells are trypsinized and lysed, and DNA is recovered from the lysate by organic extraction followed by ethanol precipitation.

Original languageEnglish
Pages (from-to)1016-1018
Number of pages3
JournalCold Spring Harbor Protocols
Volume2017
Issue number12
DOIs
Publication statusPublished - 1 Dec 2017

Cite this

Behringer, Richard ; Gertsenstein, Marina ; Nagy, Kristina Vintersten ; Nagy, Andras. / Preparation of DNA from embryonic stem cells or other cultured cells. In: Cold Spring Harbor Protocols. 2017 ; Vol. 2017, No. 12. pp. 1016-1018.
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Preparation of DNA from embryonic stem cells or other cultured cells. / Behringer, Richard; Gertsenstein, Marina; Nagy, Kristina Vintersten; Nagy, Andras.

In: Cold Spring Harbor Protocols, Vol. 2017, No. 12, 01.12.2017, p. 1016-1018.

Research output: Contribution to journalArticleOtherpeer-review

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