Preparation of Cryoprecipitate and Cryo-depleted Plasma for Proteomic Research Analysis

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Abstract

When frozen plasma is slowly thawed in cold conditions (1-6 °C), high-molecular-weight plasma proteins precipitate forming a concentrate known as cryoprecipitate. The concentrate is enriched with several important coagulation proteins, including fibrinogen, antihemophilic factor (factor VIII), von Willebrand factor, fibrin stabilizing factor (factor XIII), fibronectin, and small amounts of other plasma proteins. In current medical practice, clinical-grade preparations of cryoprecipitate are used mostly to correct fibrinogen deficiency caused by acute blood loss or due to functional abnormalities of the fibrinogen protein. In the past, cryoprecipitate was used to treat von Willebrand disease and hemophilia A (factor VIII deficiency), but the availability of more highly purified coagulation factor concentrates or recombinant protein preparations has superseded the use of cryoprecipitate for these coagulopathies. Cryo-depleted plasma (also called cryosupernatant) is the plasma supernatant remaining following removal of the cryoprecipitate from frozen-thawed plasma and contains all the remaining soluble plasma proteins. This protocol describes the research-scale preparation of cryoprecipitate and cryo-depleted plasma suitable for proteomic studies and is based on the procedures used to prepare clinical-grade cryoprecipitate.

Original languageEnglish
Title of host publicationSerum/Plasma Proteomics
Subtitle of host publicationMethods and Protocols
EditorsDavid W. Greening, Richard J. Simpson
Place of PublicationNew York NY USA
PublisherHumana Press
Chapter4
Pages41-49
Number of pages9
Volume2628
Edition3rd
ISBN (Electronic)9781071629789
ISBN (Print)9781071629772, 9781071629802
DOIs
Publication statusPublished - 2023

Publication series

NameMethods in Molecular Biology
PublisherHumana Press
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Antihemophilic factor
  • Cryo-depleted
  • Cryoprecipitate
  • Cryosupernatant
  • Factor VIII
  • Fibrinogen
  • Plasma

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