Preparation, crystallization and preliminary X-ray diffraction analysis of two intestinal fatty-acid binding proteins in the presence of 11-(dansylamino)undecanoic acid

Aisha Laguerre; Jerome Wielens; Michael Parker; Christopher Porter; Martin Scanlon

doi:10.1107/S1744309110051481

Preparation, crystallization and preliminary X-ray diffraction analysis of two intestinal fatty-acid binding proteins in the presence of 11-(dansylamino)undecanoic acid

Aisha Laguerre, Jerome Wielens, Michael Parker, Christopher Porter, Martin Scanlon

Research output: Contribution to journal › Article › Research › peer-review

4 Citations (Scopus)

Abstract

Fatty-acid binding proteins (FABPs) are abundantly expressed proteins that bind a range of lipophilic molecules. They have been implicated in the import and intracellular distribution of their ligands and have been linked with metabolic and inflammatory responses in the cells in which they are expressed. Despite their high sequence identity, human intestinal FABP (hIFABP) and rat intestinal FABP (rIFABP) bind some ligands with different affinities. In order to address the structural basis of this differential binding, diffraction-quality crystals have been obtained of hIFABP and rIFABP in complex with the fluorescent fatty-acid analogue 11-(dansylamino)undecanoic acid.

Original language	English
Pages (from-to)	291 - 295
Number of pages	5
Journal	Acta Crystallographica Section F: Structural Biology Communications
Volume	67
Issue number	2
DOIs	https://doi.org/10.1107/S1744309110051481
Publication status	Published - 2011

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10.1107/S1744309110051481

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Laguerre, A., Wielens, J., Parker, M., Porter, C., & Scanlon, M. (2011). Preparation, crystallization and preliminary X-ray diffraction analysis of two intestinal fatty-acid binding proteins in the presence of 11-(dansylamino)undecanoic acid. Acta Crystallographica Section F: Structural Biology Communications, 67(2), 291 - 295. https://doi.org/10.1107/S1744309110051481

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title = "Preparation, crystallization and preliminary X-ray diffraction analysis of two intestinal fatty-acid binding proteins in the presence of 11-(dansylamino)undecanoic acid",

abstract = "Fatty-acid binding proteins (FABPs) are abundantly expressed proteins that bind a range of lipophilic molecules. They have been implicated in the import and intracellular distribution of their ligands and have been linked with metabolic and inflammatory responses in the cells in which they are expressed. Despite their high sequence identity, human intestinal FABP (hIFABP) and rat intestinal FABP (rIFABP) bind some ligands with different affinities. In order to address the structural basis of this differential binding, diffraction-quality crystals have been obtained of hIFABP and rIFABP in complex with the fluorescent fatty-acid analogue 11-(dansylamino)undecanoic acid.",

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Laguerre, A, Wielens, J, Parker, M, Porter, C & Scanlon, M 2011, 'Preparation, crystallization and preliminary X-ray diffraction analysis of two intestinal fatty-acid binding proteins in the presence of 11-(dansylamino)undecanoic acid', Acta Crystallographica Section F: Structural Biology Communications, vol. 67, no. 2, pp. 291 - 295. https://doi.org/10.1107/S1744309110051481

Preparation, crystallization and preliminary X-ray diffraction analysis of two intestinal fatty-acid binding proteins in the presence of 11-(dansylamino)undecanoic acid. / Laguerre, Aisha; Wielens, Jerome; Parker, Michael et al.
In: Acta Crystallographica Section F: Structural Biology Communications, Vol. 67, No. 2, 2011, p. 291 - 295.

Research output: Contribution to journal › Article › Research › peer-review

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