Preparation and characterization of ZnO-CNF/chitosan hydrogel and its in vitro antibacterial activity

Janarthanan Supramaniam; Irvy Ai Xia Teh; Darren Yi Sern Low; Bey Fen Leo; Loh Teng Hern Tan; Bey Hing Goh; Sivakumar Manickam; Siah Ying Tang

doi:10.1063/5.0111070

Preparation and characterization of ZnO-CNF/chitosan hydrogel and its in vitro antibacterial activity

Janarthanan Supramaniam, Irvy Ai Xia Teh, Darren Yi Sern Low, Bey Fen Leo, Loh Teng Hern Tan, Bey Hing Goh, Sivakumar Manickam, Siah Ying Tang

Research output: Chapter in Book/Report/Conference proceeding › Conference Paper › Other

Abstract

Metallic oxide nanoparticles such as zinc oxide (ZnO) are extensively renowned for their potential use in biomedical material development. It is usually added in modern healthcare products as antibacterial agents. The fabrication of cellulose nanofibers (CNF) with ZnO is desirable due to its non-toxicity and excellent physicochemical properties. This study aims to develop flexible and microporous chitosan (CS) hydrogel film with ZnO-coated CNF nanocomposite (ZnO-CNF) via mixing and casting method to investigate its potential antibacterial activity. In this work, ZnO-CNF was obtained via a sonochemical-assisted route. The as-prepared ZnO-CNF/CS and CS hydrogel were characterized and examined using different analytical methods. Fourier transform infrared spectroscopy (FTIR) analysis revealed the strong chemical interaction between ZnO-CNF and CS. Field emission scanning electron microscopy (FESEM) observations confirmed the presence of ZnO-CNF nanocomposite on the hydrogel surface. At the same time, energy-dispersive x-ray (EDX) spectroscopy elemental mapping identified zinc specks. Further, it confirmed that ZnO-CNF were uniformly incorporated in the ZnO-CNF/CS hydrogel film. The as-synthesized ZnO-CNF/CS hydrogel film revealed antibacterial resistance against methicillin-resistant Staphylococcus aureus (MRSA) (ATCC 43300). The finding of this study offers a new route of producing a sturdy CS hydrogel film with antibacterial properties for potential wound dressing applications.

Original language	English
Title of host publication	8th Brunei International Conference on Engineering and Technology 2021
Editors	Mohammad Yeakub Ali, Rama Rao Karri, Shahriar Shams, Roslynna Rosli, Ena Kartina Abdul Rahman, Ramesh Singh
Publisher	American Institute of Physics
Number of pages	7
ISBN (Electronic)	9780735442795
DOIs	https://doi.org/10.1063/5.0111070
Publication status	Published - 10 Jan 2023
Event	Brunei International Conference on Engineering and Technology 2021 - Bandar Seri Begawan, Brunei Darussalam Duration: 8 Nov 2021 → 10 Nov 2021 Conference number: 8th https://aip.scitation.org/toc/apc/2643/1 (Proceedings)

Publication series

Name	AIP Conference Proceedings
Volume	2643
ISSN (Print)	0094-243X
ISSN (Electronic)	1551-7616

Conference

Conference	Brunei International Conference on Engineering and Technology 2021
Abbreviated title	BICET 2021
Country/Territory	Brunei Darussalam
City	Bandar Seri Begawan
Period	8/11/21 → 10/11/21
Internet address	https://aip.scitation.org/toc/apc/2643/1 (Proceedings)

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10.1063/5.0111070

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Supramaniam, J., Teh, I. A. X., Low, D. Y. S., Leo, B. F., Tan, L. T. H., Goh, B. H., Manickam, S., & Tang, S. Y. (2023). Preparation and characterization of ZnO-CNF/chitosan hydrogel and its in vitro antibacterial activity. In M. Y. Ali, R. R. Karri, S. Shams, R. Rosli, E. K. A. Rahman, & R. Singh (Eds.), 8th Brunei International Conference on Engineering and Technology 2021 Article 060018 (AIP Conference Proceedings; Vol. 2643). American Institute of Physics. https://doi.org/10.1063/5.0111070

Supramaniam, Janarthanan ; Teh, Irvy Ai Xia ; Low, Darren Yi Sern et al. / Preparation and characterization of ZnO-CNF/chitosan hydrogel and its in vitro antibacterial activity. 8th Brunei International Conference on Engineering and Technology 2021. editor / Mohammad Yeakub Ali ; Rama Rao Karri ; Shahriar Shams ; Roslynna Rosli ; Ena Kartina Abdul Rahman ; Ramesh Singh. American Institute of Physics, 2023. (AIP Conference Proceedings).

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title = "Preparation and characterization of ZnO-CNF/chitosan hydrogel and its in vitro antibacterial activity",

abstract = "Metallic oxide nanoparticles such as zinc oxide (ZnO) are extensively renowned for their potential use in biomedical material development. It is usually added in modern healthcare products as antibacterial agents. The fabrication of cellulose nanofibers (CNF) with ZnO is desirable due to its non-toxicity and excellent physicochemical properties. This study aims to develop flexible and microporous chitosan (CS) hydrogel film with ZnO-coated CNF nanocomposite (ZnO-CNF) via mixing and casting method to investigate its potential antibacterial activity. In this work, ZnO-CNF was obtained via a sonochemical-assisted route. The as-prepared ZnO-CNF/CS and CS hydrogel were characterized and examined using different analytical methods. Fourier transform infrared spectroscopy (FTIR) analysis revealed the strong chemical interaction between ZnO-CNF and CS. Field emission scanning electron microscopy (FESEM) observations confirmed the presence of ZnO-CNF nanocomposite on the hydrogel surface. At the same time, energy-dispersive x-ray (EDX) spectroscopy elemental mapping identified zinc specks. Further, it confirmed that ZnO-CNF were uniformly incorporated in the ZnO-CNF/CS hydrogel film. The as-synthesized ZnO-CNF/CS hydrogel film revealed antibacterial resistance against methicillin-resistant Staphylococcus aureus (MRSA) (ATCC 43300). The finding of this study offers a new route of producing a sturdy CS hydrogel film with antibacterial properties for potential wound dressing applications.",

author = "Janarthanan Supramaniam and Teh, {Irvy Ai Xia} and Low, {Darren Yi Sern} and Leo, {Bey Fen} and Tan, {Loh Teng Hern} and Goh, {Bey Hing} and Sivakumar Manickam and Tang, {Siah Ying}",

note = "Funding Information: This work was supported by the Tropical Medicine and Biology Platform, School of Science, Monash University Malaysia. Publisher Copyright: {\textcopyright} 2023 American Institute of Physics Inc.. All rights reserved.; Brunei International Conference on Engineering and Technology 2021, BICET 2021 ; Conference date: 08-11-2021 Through 10-11-2021",

year = "2023",

month = jan,

day = "10",

doi = "10.1063/5.0111070",

language = "English",

series = "AIP Conference Proceedings",

publisher = "American Institute of Physics",

editor = "Ali, {Mohammad Yeakub} and Karri, {Rama Rao} and Shahriar Shams and Roslynna Rosli and Rahman, {Ena Kartina Abdul} and Ramesh Singh",

booktitle = "8th Brunei International Conference on Engineering and Technology 2021",

address = "United States of America",

url = "https://aip.scitation.org/toc/apc/2643/1",

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Supramaniam, J, Teh, IAX, Low, DYS, Leo, BF, Tan, LTH, Goh, BH, Manickam, S & Tang, SY 2023, Preparation and characterization of ZnO-CNF/chitosan hydrogel and its in vitro antibacterial activity. in MY Ali, RR Karri, S Shams, R Rosli, EKA Rahman & R Singh (eds), 8th Brunei International Conference on Engineering and Technology 2021., 060018, AIP Conference Proceedings, vol. 2643, American Institute of Physics, Brunei International Conference on Engineering and Technology 2021, Bandar Seri Begawan, Brunei Darussalam, 8/11/21. https://doi.org/10.1063/5.0111070

Preparation and characterization of ZnO-CNF/chitosan hydrogel and its in vitro antibacterial activity. / Supramaniam, Janarthanan; Teh, Irvy Ai Xia; Low, Darren Yi Sern et al.
8th Brunei International Conference on Engineering and Technology 2021. ed. / Mohammad Yeakub Ali; Rama Rao Karri; Shahriar Shams; Roslynna Rosli; Ena Kartina Abdul Rahman; Ramesh Singh. American Institute of Physics, 2023. 060018 (AIP Conference Proceedings; Vol. 2643).

Research output: Chapter in Book/Report/Conference proceeding › Conference Paper › Other

TY - GEN

T1 - Preparation and characterization of ZnO-CNF/chitosan hydrogel and its in vitro antibacterial activity

AU - Supramaniam, Janarthanan

AU - Teh, Irvy Ai Xia

AU - Low, Darren Yi Sern

AU - Leo, Bey Fen

AU - Tan, Loh Teng Hern

AU - Goh, Bey Hing

AU - Manickam, Sivakumar

AU - Tang, Siah Ying

N1 - Conference code: 8th

PY - 2023/1/10

Y1 - 2023/1/10

N2 - Metallic oxide nanoparticles such as zinc oxide (ZnO) are extensively renowned for their potential use in biomedical material development. It is usually added in modern healthcare products as antibacterial agents. The fabrication of cellulose nanofibers (CNF) with ZnO is desirable due to its non-toxicity and excellent physicochemical properties. This study aims to develop flexible and microporous chitosan (CS) hydrogel film with ZnO-coated CNF nanocomposite (ZnO-CNF) via mixing and casting method to investigate its potential antibacterial activity. In this work, ZnO-CNF was obtained via a sonochemical-assisted route. The as-prepared ZnO-CNF/CS and CS hydrogel were characterized and examined using different analytical methods. Fourier transform infrared spectroscopy (FTIR) analysis revealed the strong chemical interaction between ZnO-CNF and CS. Field emission scanning electron microscopy (FESEM) observations confirmed the presence of ZnO-CNF nanocomposite on the hydrogel surface. At the same time, energy-dispersive x-ray (EDX) spectroscopy elemental mapping identified zinc specks. Further, it confirmed that ZnO-CNF were uniformly incorporated in the ZnO-CNF/CS hydrogel film. The as-synthesized ZnO-CNF/CS hydrogel film revealed antibacterial resistance against methicillin-resistant Staphylococcus aureus (MRSA) (ATCC 43300). The finding of this study offers a new route of producing a sturdy CS hydrogel film with antibacterial properties for potential wound dressing applications.

AB - Metallic oxide nanoparticles such as zinc oxide (ZnO) are extensively renowned for their potential use in biomedical material development. It is usually added in modern healthcare products as antibacterial agents. The fabrication of cellulose nanofibers (CNF) with ZnO is desirable due to its non-toxicity and excellent physicochemical properties. This study aims to develop flexible and microporous chitosan (CS) hydrogel film with ZnO-coated CNF nanocomposite (ZnO-CNF) via mixing and casting method to investigate its potential antibacterial activity. In this work, ZnO-CNF was obtained via a sonochemical-assisted route. The as-prepared ZnO-CNF/CS and CS hydrogel were characterized and examined using different analytical methods. Fourier transform infrared spectroscopy (FTIR) analysis revealed the strong chemical interaction between ZnO-CNF and CS. Field emission scanning electron microscopy (FESEM) observations confirmed the presence of ZnO-CNF nanocomposite on the hydrogel surface. At the same time, energy-dispersive x-ray (EDX) spectroscopy elemental mapping identified zinc specks. Further, it confirmed that ZnO-CNF were uniformly incorporated in the ZnO-CNF/CS hydrogel film. The as-synthesized ZnO-CNF/CS hydrogel film revealed antibacterial resistance against methicillin-resistant Staphylococcus aureus (MRSA) (ATCC 43300). The finding of this study offers a new route of producing a sturdy CS hydrogel film with antibacterial properties for potential wound dressing applications.

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U2 - 10.1063/5.0111070

DO - 10.1063/5.0111070

M3 - Conference Paper

AN - SCOPUS:85146530221

T3 - AIP Conference Proceedings

BT - 8th Brunei International Conference on Engineering and Technology 2021

A2 - Ali, Mohammad Yeakub

A2 - Karri, Rama Rao

A2 - Shams, Shahriar

A2 - Rosli, Roslynna

A2 - Rahman, Ena Kartina Abdul

A2 - Singh, Ramesh

PB - American Institute of Physics

T2 - Brunei International Conference on Engineering and Technology 2021

Y2 - 8 November 2021 through 10 November 2021

ER -

Supramaniam J, Teh IAX, Low DYS, Leo BF, Tan LTH, Goh BH et al. Preparation and characterization of ZnO-CNF/chitosan hydrogel and its in vitro antibacterial activity. In Ali MY, Karri RR, Shams S, Rosli R, Rahman EKA, Singh R, editors, 8th Brunei International Conference on Engineering and Technology 2021. American Institute of Physics. 2023. 060018. (AIP Conference Proceedings). doi: 10.1063/5.0111070

Preparation and characterization of ZnO-CNF/chitosan hydrogel and its in vitro antibacterial activity

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