Precocious terminal differentiation of premigratory limb muscle precursor cells requires positive signalling

Sara J Ventner, Rebecca E Argent, Fiona M Deegan, Gina Perez-Baron, Ted S Wong, William E Tidyman, Wilfred F Denetclaw Jr., Christophe Marcelle, Marianne Bronner-Fraser, Charles P Ordahl

Research output: Contribution to journalArticleResearchpeer-review

Abstract

The timing of myogenic differentiation of hypaxial muscle precursor cells in the somite lags behind that of epaxial precursors. Two hypotheses have been proposed to explain this delay. One attributes the delay to the presence of negative-acting signals from the lateral plate mesoderm adjacent to the hypaxial muscle precursor cells located in the ventrolateral lip of the somitic dermomyotome (Pourquie et al. [1995] Proc. Natl. Acad. Sci. USA 92:3219-3223). The second attributes the delay to an absence of positive-acting inductive signals, similar to those from the axial structures that induce epaxial myotome development (Pownall et al. [1996] Development 122:1475-1488). Because both studies relied principally upon changes in the expression pattern of mRNAs specific to early muscle precursor cell markers, we revisited these experiments using two methods to assess muscle terminal differentiation. First, injection of fluorescent dyes before surgery was used to determine whether ventrolateral lip cells transform from epithelial cells to elongated myocytes. Second, an antibody to a terminal differentiation marker and a new monoclonal antibody that recognises avian and mammalian Pax3 were used for immunohistochemistry to assess the transition from precursor cell to myocyte. The results support both hypotheses and show further that placing axial structures adjacent to the somite ventrolateral lip induces an axial pattern of myocyte terminal differentiation and elongation.
Original languageEnglish
Pages (from-to)591 - 599
Number of pages9
JournalDevelopmental Dynamics
Volume229
Issue number3
Publication statusPublished - 2004

Cite this

Ventner, S. J., Argent, R. E., Deegan, F. M., Perez-Baron, G., Wong, T. S., Tidyman, W. E., ... Ordahl, C. P. (2004). Precocious terminal differentiation of premigratory limb muscle precursor cells requires positive signalling. Developmental Dynamics, 229(3), 591 - 599.
Ventner, Sara J ; Argent, Rebecca E ; Deegan, Fiona M ; Perez-Baron, Gina ; Wong, Ted S ; Tidyman, William E ; Denetclaw Jr., Wilfred F ; Marcelle, Christophe ; Bronner-Fraser, Marianne ; Ordahl, Charles P. / Precocious terminal differentiation of premigratory limb muscle precursor cells requires positive signalling. In: Developmental Dynamics. 2004 ; Vol. 229, No. 3. pp. 591 - 599.
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abstract = "The timing of myogenic differentiation of hypaxial muscle precursor cells in the somite lags behind that of epaxial precursors. Two hypotheses have been proposed to explain this delay. One attributes the delay to the presence of negative-acting signals from the lateral plate mesoderm adjacent to the hypaxial muscle precursor cells located in the ventrolateral lip of the somitic dermomyotome (Pourquie et al. [1995] Proc. Natl. Acad. Sci. USA 92:3219-3223). The second attributes the delay to an absence of positive-acting inductive signals, similar to those from the axial structures that induce epaxial myotome development (Pownall et al. [1996] Development 122:1475-1488). Because both studies relied principally upon changes in the expression pattern of mRNAs specific to early muscle precursor cell markers, we revisited these experiments using two methods to assess muscle terminal differentiation. First, injection of fluorescent dyes before surgery was used to determine whether ventrolateral lip cells transform from epithelial cells to elongated myocytes. Second, an antibody to a terminal differentiation marker and a new monoclonal antibody that recognises avian and mammalian Pax3 were used for immunohistochemistry to assess the transition from precursor cell to myocyte. The results support both hypotheses and show further that placing axial structures adjacent to the somite ventrolateral lip induces an axial pattern of myocyte terminal differentiation and elongation.",
author = "Ventner, {Sara J} and Argent, {Rebecca E} and Deegan, {Fiona M} and Gina Perez-Baron and Wong, {Ted S} and Tidyman, {William E} and {Denetclaw Jr.}, {Wilfred F} and Christophe Marcelle and Marianne Bronner-Fraser and Ordahl, {Charles P}",
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Ventner, SJ, Argent, RE, Deegan, FM, Perez-Baron, G, Wong, TS, Tidyman, WE, Denetclaw Jr., WF, Marcelle, C, Bronner-Fraser, M & Ordahl, CP 2004, 'Precocious terminal differentiation of premigratory limb muscle precursor cells requires positive signalling' Developmental Dynamics, vol. 229, no. 3, pp. 591 - 599.

Precocious terminal differentiation of premigratory limb muscle precursor cells requires positive signalling. / Ventner, Sara J; Argent, Rebecca E; Deegan, Fiona M; Perez-Baron, Gina; Wong, Ted S; Tidyman, William E; Denetclaw Jr., Wilfred F; Marcelle, Christophe; Bronner-Fraser, Marianne; Ordahl, Charles P.

In: Developmental Dynamics, Vol. 229, No. 3, 2004, p. 591 - 599.

Research output: Contribution to journalArticleResearchpeer-review

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T1 - Precocious terminal differentiation of premigratory limb muscle precursor cells requires positive signalling

AU - Ventner, Sara J

AU - Argent, Rebecca E

AU - Deegan, Fiona M

AU - Perez-Baron, Gina

AU - Wong, Ted S

AU - Tidyman, William E

AU - Denetclaw Jr., Wilfred F

AU - Marcelle, Christophe

AU - Bronner-Fraser, Marianne

AU - Ordahl, Charles P

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N2 - The timing of myogenic differentiation of hypaxial muscle precursor cells in the somite lags behind that of epaxial precursors. Two hypotheses have been proposed to explain this delay. One attributes the delay to the presence of negative-acting signals from the lateral plate mesoderm adjacent to the hypaxial muscle precursor cells located in the ventrolateral lip of the somitic dermomyotome (Pourquie et al. [1995] Proc. Natl. Acad. Sci. USA 92:3219-3223). The second attributes the delay to an absence of positive-acting inductive signals, similar to those from the axial structures that induce epaxial myotome development (Pownall et al. [1996] Development 122:1475-1488). Because both studies relied principally upon changes in the expression pattern of mRNAs specific to early muscle precursor cell markers, we revisited these experiments using two methods to assess muscle terminal differentiation. First, injection of fluorescent dyes before surgery was used to determine whether ventrolateral lip cells transform from epithelial cells to elongated myocytes. Second, an antibody to a terminal differentiation marker and a new monoclonal antibody that recognises avian and mammalian Pax3 were used for immunohistochemistry to assess the transition from precursor cell to myocyte. The results support both hypotheses and show further that placing axial structures adjacent to the somite ventrolateral lip induces an axial pattern of myocyte terminal differentiation and elongation.

AB - The timing of myogenic differentiation of hypaxial muscle precursor cells in the somite lags behind that of epaxial precursors. Two hypotheses have been proposed to explain this delay. One attributes the delay to the presence of negative-acting signals from the lateral plate mesoderm adjacent to the hypaxial muscle precursor cells located in the ventrolateral lip of the somitic dermomyotome (Pourquie et al. [1995] Proc. Natl. Acad. Sci. USA 92:3219-3223). The second attributes the delay to an absence of positive-acting inductive signals, similar to those from the axial structures that induce epaxial myotome development (Pownall et al. [1996] Development 122:1475-1488). Because both studies relied principally upon changes in the expression pattern of mRNAs specific to early muscle precursor cell markers, we revisited these experiments using two methods to assess muscle terminal differentiation. First, injection of fluorescent dyes before surgery was used to determine whether ventrolateral lip cells transform from epithelial cells to elongated myocytes. Second, an antibody to a terminal differentiation marker and a new monoclonal antibody that recognises avian and mammalian Pax3 were used for immunohistochemistry to assess the transition from precursor cell to myocyte. The results support both hypotheses and show further that placing axial structures adjacent to the somite ventrolateral lip induces an axial pattern of myocyte terminal differentiation and elongation.

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Ventner SJ, Argent RE, Deegan FM, Perez-Baron G, Wong TS, Tidyman WE et al. Precocious terminal differentiation of premigratory limb muscle precursor cells requires positive signalling. Developmental Dynamics. 2004;229(3):591 - 599.