Pre-targeting amyloid-β with antibodies for potential molecular imaging of Alzheimer's disease

Katherine A. Morgan; Michael de Veer; Luke A. Miles; Cormac A.A. Kelderman; Catriona A. McLean; Colin L. Masters; Kevin J. Barnham; Jonathan M. White; Brett M. Paterson; Paul S. Donnelly

doi:10.1039/d2cc06850h

Pre-targeting amyloid-β with antibodies for potential molecular imaging of Alzheimer's disease

Katherine A. Morgan, Michael de Veer, Luke A. Miles, Cormac A.A. Kelderman, Catriona A. McLean, Colin L. Masters, Kevin J. Barnham, Jonathan M. White, Brett M. Paterson, Paul S. Donnelly

Research output: Contribution to journal › Article › Research › peer-review

5 Citations (Scopus)

Abstract

With the aim of developing the concept of pretargeted click chemistry for the diagnosis of Alzheimer's disease two antibodies specific for amyloid-β were modified to incorporate trans-cyclooctene functional groups. Two bis(thiosemicarbazone) compounds with pendant 1,2,4,5-tetrazine functional groups were prepared and radiolabelled with positron emitting copper-64. The new copper-64 complexes rapidly react with the trans-cyclooctene functionalized antibodies in a bioorthogonal click reaction and cross the blood-brain barrier in mice.

Original language	English
Pages (from-to)	2243-2246
Number of pages	4
Journal	Chemical Communications
Volume	59
Issue number	16
DOIs	https://doi.org/10.1039/d2cc06850h
Publication status	Published - 17 Jan 2023

Access to Document

10.1039/d2cc06850h

Cite this

@article{a04da0a8cd8c4777b84dab80f9b9d182,

title = "Pre-targeting amyloid-β with antibodies for potential molecular imaging of Alzheimer's disease",

abstract = "With the aim of developing the concept of pretargeted click chemistry for the diagnosis of Alzheimer's disease two antibodies specific for amyloid-β were modified to incorporate trans-cyclooctene functional groups. Two bis(thiosemicarbazone) compounds with pendant 1,2,4,5-tetrazine functional groups were prepared and radiolabelled with positron emitting copper-64. The new copper-64 complexes rapidly react with the trans-cyclooctene functionalized antibodies in a bioorthogonal click reaction and cross the blood-brain barrier in mice.",

author = "Morgan, {Katherine A.} and {de Veer}, Michael and Miles, {Luke A.} and Kelderman, {Cormac A.A.} and McLean, {Catriona A.} and Masters, {Colin L.} and Barnham, {Kevin J.} and White, {Jonathan M.} and Paterson, {Brett M.} and Donnelly, {Paul S.}",

note = "Publisher Copyright: {\textcopyright} 2023 The Royal Society of Chemistry.",

year = "2023",

month = jan,

day = "17",

doi = "10.1039/d2cc06850h",

language = "English",

volume = "59",

pages = "2243--2246",

journal = "Chemical Communications",

issn = "1359-7345",

publisher = "The Royal Society of Chemistry",

number = "16",

}

TY - JOUR

T1 - Pre-targeting amyloid-β with antibodies for potential molecular imaging of Alzheimer's disease

AU - Morgan, Katherine A.

AU - de Veer, Michael

AU - Miles, Luke A.

AU - Kelderman, Cormac A.A.

AU - McLean, Catriona A.

AU - Masters, Colin L.

AU - Barnham, Kevin J.

AU - White, Jonathan M.

AU - Paterson, Brett M.

AU - Donnelly, Paul S.

PY - 2023/1/17

Y1 - 2023/1/17

N2 - With the aim of developing the concept of pretargeted click chemistry for the diagnosis of Alzheimer's disease two antibodies specific for amyloid-β were modified to incorporate trans-cyclooctene functional groups. Two bis(thiosemicarbazone) compounds with pendant 1,2,4,5-tetrazine functional groups were prepared and radiolabelled with positron emitting copper-64. The new copper-64 complexes rapidly react with the trans-cyclooctene functionalized antibodies in a bioorthogonal click reaction and cross the blood-brain barrier in mice.

AB - With the aim of developing the concept of pretargeted click chemistry for the diagnosis of Alzheimer's disease two antibodies specific for amyloid-β were modified to incorporate trans-cyclooctene functional groups. Two bis(thiosemicarbazone) compounds with pendant 1,2,4,5-tetrazine functional groups were prepared and radiolabelled with positron emitting copper-64. The new copper-64 complexes rapidly react with the trans-cyclooctene functionalized antibodies in a bioorthogonal click reaction and cross the blood-brain barrier in mice.

UR - http://www.scopus.com/inward/record.url?scp=85147441556&partnerID=8YFLogxK

U2 - 10.1039/d2cc06850h

DO - 10.1039/d2cc06850h

M3 - Article

C2 - 36723107

AN - SCOPUS:85147441556

SN - 1359-7345

VL - 59

SP - 2243

EP - 2246

JO - Chemical Communications

JF - Chemical Communications

IS - 16

ER -

Pre-targeting amyloid-β with antibodies for potential molecular imaging of Alzheimer's disease

Abstract

Access to Document

Other files and links

Monash Biomedical Imaging (MBI)

Cite this

Pre-targeting amyloid-β with antibodies for potential molecular imaging of Alzheimer's disease

Abstract

Access to Document

Other files and links

Equipment

Monash Biomedical Imaging (MBI)

Cite this