Abstract
Background: Defining how epigenetic information is established in the germline during fetal development is key to understanding how epigenetic information is inherited and impacts on evolution and human health and disease. Results: Here, we show that Polycomb Repressive Complex 2 is transiently localized in the nucleus of mouse fetal germ cells, while DNA methylation is removed from the germline. This coincides with significant enrichment of trimethylated lysine 27 on histone 3 near the nuclear lamina that is dependent on activity of the essential PRC2 catalytic proteins, Enhancer of Zeste 1 and/or 2. Conclusions: Combined, these data reveal a role for Polycomb Repressive Complex 2 and trimethylated lysine 27 on histone 3 during germline epigenetic programming that we speculate is required to repress target sequences while DNA methylation is removed.
Original language | English |
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Article number | 7 |
Number of pages | 20 |
Journal | Epigenetics and Chromatin |
Volume | 10 |
Issue number | 1 |
DOIs | |
Publication status | Published - 20 Feb 2017 |
Keywords
- Epigenetic reprogramming
- Epigenetics
- Germ cells
- H3K27me3
- Histone modifications
Equipment
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Animal Research Platform (MARP)
John Phelps (Manager)
Office of the Vice-Provost (Research and Research Infrastructure)Facility/equipment: Facility
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MHTP Medical Genomics Facility
Vivien Vasic (Manager)
Office of the Vice-Provost (Research and Research Infrastructure)Facility/equipment: Facility
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Monash Micro Imaging
Ian Harper (Manager), Stephen Firth (Manager), Alex Fulcher (Operator), Oleks Chernyavskiy (Operator), Margaret Rzeszutek (Other), David Potter (Manager), Volker Hilsenstein (Operator), Juan Nunez-Iglesias (Other), Stephen Cody (Manager), Irena Carmichael (Operator), Betty Kouskousis (Other), Chad Johnson (Operator), Sarah Creed (Manager) & Giulia Ballerin (Operator)
Office of the Vice-Provost (Research and Research Infrastructure)Facility/equipment: Facility