Practical and low cost whole-organism motility assay: a step-by-step protocol

Sarah Preston, Abdul Jabbar, Cameron Nowell, Anja Joachim, Baerbel Ruttkowski, Tony Cardno, Andreas Hofmann, Robin B. Gasser

Research output: Contribution to journalArticleOtherpeer-review

19 Citations (Scopus)

Abstract

Here, we provide a step-by-step protocol for a practical and low cost whole-organism assay for the screening of chemical compounds for activity against parasitic worms. This assay has considerable advantages over conventional methods, mainly in relation to ease of use, throughput, time and cost. It is readily suited to the screening of hundreds to thousands of compounds for subsequent hit-to-lead optimisation, and should be applicable to many different parasites and other organisms commensurate with the size of wells in the microtiter plates used for phenotypic screening.

Original languageEnglish
Pages (from-to)13-17
Number of pages5
JournalMolecular and Cellular Probes
Volume30
Issue number1
DOIs
Publication statusPublished - 1 Feb 2016

Keywords

  • Drug screening
  • Motility assay
  • Nematode
  • Parasite
  • Step-by-step protocol

Cite this

Preston, S., Jabbar, A., Nowell, C., Joachim, A., Ruttkowski, B., Cardno, T., Hofmann, A., & Gasser, R. B. (2016). Practical and low cost whole-organism motility assay: a step-by-step protocol. Molecular and Cellular Probes, 30(1), 13-17. https://doi.org/10.1016/j.mcp.2015.08.005