TY - JOUR
T1 - Potential of curcumin-loaded cubosomes for topical treatment of cervical cancer
AU - Victorelli, Francesca Damiani
AU - Salvati Manni, Livia
AU - Biffi, Stefania
AU - Bortot, Barbara
AU - Buzzá, Hilde Harb
AU - Lutz-Bueno, Viviane
AU - Handschin, Stephan
AU - Calixto, Giovana
AU - Murgia, Sergio
AU - Chorilli, Marlus
AU - Mezzenga, Raffaele
N1 - Funding Information:
The authors gratefully acknowledge Mario Salviato - Ovos férteis Porto Ferreira/SP, Brazil and AD'ORO S.A., São Carlos/SP, Brazil for providing the eggs for this research, Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), and Programa de Apoio ao Desenvolvimento Científico (PADC) and the National Institute of Science and Technology in Pharmaceutical Nanotechnology: a transdisciplinary approach INCT-NANOFARMA, which is supported by the São Paulo Research Foundation (FAPESP, Brazil) Grants \#2017/23357-2, \#2019/03929-7, \#2019/07245-5. The authors also thank FAPESP for the grants \#2016/14033-6, \#2013/07276-1 and \#2014/50857-8 and CNPQ grant \#465360/2014-9. The authors also acknowledge the support from ScopeM/Swiss Federal Institute of Technology, ETHZ. The authors are grateful to the company, Biorender. Some illustrations were prepared with Biorender.com.
Funding Information:
The authors gratefully acknowledge Mario Salviato - Ovos f?rteis Porto Ferreira/SP, Brazil and AD'ORO S.A. S?o Carlos/SP, Brazil for providing the eggs for this research, Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico (CNPq), and Programa de Apoio ao Desenvolvimento Cient?fico (PADC) and the National Institute of Science and Technology in Pharmaceutical Nanotechnology: a transdisciplinary approach INCT-NANOFARMA, which is supported by the S?o Paulo Research Foundation (FAPESP, Brazil) Grants \#2017/23357-2, \#2019/03929-7, \#2019/07245-5. The authors also thank FAPESP for the grants \#2016/14033-6, \#2013/07276-1 and \#2014/50857-8 and CNPQ grant \#465360/2014-9. The authors also acknowledge the support from ScopeM/Swiss Federal Institute of Technology, ETHZ. The authors are grateful to the company, Biorender. Some illustrations were prepared with Biorender.com. Data are available from the corresponding author upon reasonable request.
Publisher Copyright:
© 2022 The Author(s)
PY - 2022/8/15
Y1 - 2022/8/15
N2 - Cervical cancer is one of the most common cancers affecting women worldwide. There are an estimated 570,000 new cases of cervical cancer each year and conventional treatments can cause severe side effects. In this work, we developed a platform for vaginal administration of lipophilic drugs for cervical cancer treatment. We formulated mucoadhesive cubosomes for the delivery of curcumin, a lipophilic drug for cervical cancer treatment, to increase its bioavailability and local absorption. This study tests the use of cubosomes for vaginal drug administration and assesses their potential efficiency using the CAM (chick embryo chorioallantoic membrane) model. SAXS (small-angle X-ray scattering), cryo-TEM (cryo-transmission electron microscopy), and dynamic light scattering (DLS) were employed to characterise the system. With ex vivo permeation and retention studies, we find that the curcumin released from our system is retained in the vaginal mucosa. In vitro cytotoxicity assay and cellular uptake showed an increased cytotoxic effect of curcumin against HeLa cell line when incorporated into the cubosomes. The curcumin-loaded cubosomes also demonstrated an antiangiogenic effect evaluated in vivo by the CAM model.
AB - Cervical cancer is one of the most common cancers affecting women worldwide. There are an estimated 570,000 new cases of cervical cancer each year and conventional treatments can cause severe side effects. In this work, we developed a platform for vaginal administration of lipophilic drugs for cervical cancer treatment. We formulated mucoadhesive cubosomes for the delivery of curcumin, a lipophilic drug for cervical cancer treatment, to increase its bioavailability and local absorption. This study tests the use of cubosomes for vaginal drug administration and assesses their potential efficiency using the CAM (chick embryo chorioallantoic membrane) model. SAXS (small-angle X-ray scattering), cryo-TEM (cryo-transmission electron microscopy), and dynamic light scattering (DLS) were employed to characterise the system. With ex vivo permeation and retention studies, we find that the curcumin released from our system is retained in the vaginal mucosa. In vitro cytotoxicity assay and cellular uptake showed an increased cytotoxic effect of curcumin against HeLa cell line when incorporated into the cubosomes. The curcumin-loaded cubosomes also demonstrated an antiangiogenic effect evaluated in vivo by the CAM model.
KW - Cervical cancer
KW - Chorioallantoic membrane model
KW - Cubosomes
KW - DOTAP
KW - Mucoadhesion
KW - Vaginal administration
UR - http://www.scopus.com/inward/record.url?scp=85128415663&partnerID=8YFLogxK
U2 - 10.1016/j.jcis.2022.04.031
DO - 10.1016/j.jcis.2022.04.031
M3 - Article
C2 - 35439693
AN - SCOPUS:85128415663
SN - 0021-9797
VL - 620
SP - 419
EP - 430
JO - Journal of Colloid and Interface Science
JF - Journal of Colloid and Interface Science
ER -
