Population genomics of hypervirulent Klebsiella pneumoniae clonal-group 23 reveals early emergence and rapid global dissemination

Margaret M.C. Lam, Kelly L. Wyres, Sebastian Duchêne, Ryan R. Wick, Louise M. Judd, Yunn Hwen Gan, Chu Han Hoh, Sophia Archuleta, James S. Molton, Shirin Kalimuddin, Tse Hsien Koh, Virginie Passet, Sylvain Brisse, Kathryn E. Holt

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Severe liver abscess infections caused by hypervirulent clonal-group CG23 Klebsiella pneumoniae have been increasingly reported since the mid-1980s. Strains typically possess several virulence factors including an integrative, conjugative element ICEKp encoding the siderophore yersiniabactin and genotoxin colibactin. Here we investigate CG23's evolutionary history, showing several deep-branching sublineages associated with distinct ICEKp acquisitions. Over 80% of liver abscess isolates belong to sublineage CG23-I, which emerged in ∼1928 following acquisition of ICEKp10 (encoding yersiniabactin and colibactin), and then disseminated globally within the human population. CG23-I's distinguishing feature is the colibactin synthesis locus, which reportedly promotes gut colonisation and metastatic infection in murine models. These data show circulation of CG23 K. pneumoniae decades before the liver abscess epidemic was first recognised, and provide a framework for future epidemiological and experimental studies of hypervirulent K. pneumoniae. To support such studies we present an open access, completely sequenced CG23-I human liver abscess isolate, SGH10.

Original languageEnglish
Article number2703
Number of pages10
JournalNature Communications
Volume9
Issue number1
DOIs
Publication statusPublished - 1 Dec 2018
Externally publishedYes

Cite this

Lam, Margaret M.C. ; Wyres, Kelly L. ; Duchêne, Sebastian ; Wick, Ryan R. ; Judd, Louise M. ; Gan, Yunn Hwen ; Hoh, Chu Han ; Archuleta, Sophia ; Molton, James S. ; Kalimuddin, Shirin ; Koh, Tse Hsien ; Passet, Virginie ; Brisse, Sylvain ; Holt, Kathryn E. / Population genomics of hypervirulent Klebsiella pneumoniae clonal-group 23 reveals early emergence and rapid global dissemination. In: Nature Communications. 2018 ; Vol. 9, No. 1.
@article{4d15f2a6b89c45c7a8322461d66e98a3,
title = "Population genomics of hypervirulent Klebsiella pneumoniae clonal-group 23 reveals early emergence and rapid global dissemination",
abstract = "Severe liver abscess infections caused by hypervirulent clonal-group CG23 Klebsiella pneumoniae have been increasingly reported since the mid-1980s. Strains typically possess several virulence factors including an integrative, conjugative element ICEKp encoding the siderophore yersiniabactin and genotoxin colibactin. Here we investigate CG23's evolutionary history, showing several deep-branching sublineages associated with distinct ICEKp acquisitions. Over 80{\%} of liver abscess isolates belong to sublineage CG23-I, which emerged in ∼1928 following acquisition of ICEKp10 (encoding yersiniabactin and colibactin), and then disseminated globally within the human population. CG23-I's distinguishing feature is the colibactin synthesis locus, which reportedly promotes gut colonisation and metastatic infection in murine models. These data show circulation of CG23 K. pneumoniae decades before the liver abscess epidemic was first recognised, and provide a framework for future epidemiological and experimental studies of hypervirulent K. pneumoniae. To support such studies we present an open access, completely sequenced CG23-I human liver abscess isolate, SGH10.",
author = "Lam, {Margaret M.C.} and Wyres, {Kelly L.} and Sebastian Duch{\^e}ne and Wick, {Ryan R.} and Judd, {Louise M.} and Gan, {Yunn Hwen} and Hoh, {Chu Han} and Sophia Archuleta and Molton, {James S.} and Shirin Kalimuddin and Koh, {Tse Hsien} and Virginie Passet and Sylvain Brisse and Holt, {Kathryn E.}",
year = "2018",
month = "12",
day = "1",
doi = "10.1038/s41467-018-05114-7",
language = "English",
volume = "9",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "Nature Publishing Group",
number = "1",

}

Lam, MMC, Wyres, KL, Duchêne, S, Wick, RR, Judd, LM, Gan, YH, Hoh, CH, Archuleta, S, Molton, JS, Kalimuddin, S, Koh, TH, Passet, V, Brisse, S & Holt, KE 2018, 'Population genomics of hypervirulent Klebsiella pneumoniae clonal-group 23 reveals early emergence and rapid global dissemination' Nature Communications, vol. 9, no. 1, 2703. https://doi.org/10.1038/s41467-018-05114-7

Population genomics of hypervirulent Klebsiella pneumoniae clonal-group 23 reveals early emergence and rapid global dissemination. / Lam, Margaret M.C.; Wyres, Kelly L.; Duchêne, Sebastian; Wick, Ryan R.; Judd, Louise M.; Gan, Yunn Hwen; Hoh, Chu Han; Archuleta, Sophia; Molton, James S.; Kalimuddin, Shirin; Koh, Tse Hsien; Passet, Virginie; Brisse, Sylvain; Holt, Kathryn E.

In: Nature Communications, Vol. 9, No. 1, 2703, 01.12.2018.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Population genomics of hypervirulent Klebsiella pneumoniae clonal-group 23 reveals early emergence and rapid global dissemination

AU - Lam, Margaret M.C.

AU - Wyres, Kelly L.

AU - Duchêne, Sebastian

AU - Wick, Ryan R.

AU - Judd, Louise M.

AU - Gan, Yunn Hwen

AU - Hoh, Chu Han

AU - Archuleta, Sophia

AU - Molton, James S.

AU - Kalimuddin, Shirin

AU - Koh, Tse Hsien

AU - Passet, Virginie

AU - Brisse, Sylvain

AU - Holt, Kathryn E.

PY - 2018/12/1

Y1 - 2018/12/1

N2 - Severe liver abscess infections caused by hypervirulent clonal-group CG23 Klebsiella pneumoniae have been increasingly reported since the mid-1980s. Strains typically possess several virulence factors including an integrative, conjugative element ICEKp encoding the siderophore yersiniabactin and genotoxin colibactin. Here we investigate CG23's evolutionary history, showing several deep-branching sublineages associated with distinct ICEKp acquisitions. Over 80% of liver abscess isolates belong to sublineage CG23-I, which emerged in ∼1928 following acquisition of ICEKp10 (encoding yersiniabactin and colibactin), and then disseminated globally within the human population. CG23-I's distinguishing feature is the colibactin synthesis locus, which reportedly promotes gut colonisation and metastatic infection in murine models. These data show circulation of CG23 K. pneumoniae decades before the liver abscess epidemic was first recognised, and provide a framework for future epidemiological and experimental studies of hypervirulent K. pneumoniae. To support such studies we present an open access, completely sequenced CG23-I human liver abscess isolate, SGH10.

AB - Severe liver abscess infections caused by hypervirulent clonal-group CG23 Klebsiella pneumoniae have been increasingly reported since the mid-1980s. Strains typically possess several virulence factors including an integrative, conjugative element ICEKp encoding the siderophore yersiniabactin and genotoxin colibactin. Here we investigate CG23's evolutionary history, showing several deep-branching sublineages associated with distinct ICEKp acquisitions. Over 80% of liver abscess isolates belong to sublineage CG23-I, which emerged in ∼1928 following acquisition of ICEKp10 (encoding yersiniabactin and colibactin), and then disseminated globally within the human population. CG23-I's distinguishing feature is the colibactin synthesis locus, which reportedly promotes gut colonisation and metastatic infection in murine models. These data show circulation of CG23 K. pneumoniae decades before the liver abscess epidemic was first recognised, and provide a framework for future epidemiological and experimental studies of hypervirulent K. pneumoniae. To support such studies we present an open access, completely sequenced CG23-I human liver abscess isolate, SGH10.

UR - http://www.scopus.com/inward/record.url?scp=85049930500&partnerID=8YFLogxK

U2 - 10.1038/s41467-018-05114-7

DO - 10.1038/s41467-018-05114-7

M3 - Article

VL - 9

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

IS - 1

M1 - 2703

ER -