Abstract
A phosphate biosensor based on potentiometric detection mode is described. Purine nucleoside phosphorylase (PNP) and xanthine oxidase (XOD) were immobilised into polypyrrole films for potentiometric measurement of phosphate in 0.05 M barbitone buffer (pH 7.8) which contain 10 mM inosine. A minimum detectable amount of 1.0 I?M phosphate and a linear concentration range of 5a??25 I?M were achieved enabling sensitive potentiometric detection and a wide linear concentration range. The presence of uric and ascorbic acids had the least effect on the performance of the PPya??PNPa??XODa??Fe(CN)64a?? biosensor and therefore will not have any effect on phosphate measurement at levels normally present in water.
Original language | English |
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Pages (from-to) | 45 - 49 |
Number of pages | 5 |
Journal | Journal of Molecular Catalysis B: Enzymatic |
Volume | 63 |
Issue number | 1-2 |
DOIs | |
Publication status | Published - 2010 |