Polymer-Protein/Peptide Bioconjugates

Paul Wilson, Julien Nicolas, David M. Haddleton

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Researchpeer-review

6 Citations (Scopus)

Abstract

This chapter discusses the main synthetic strategies to achieve polymer-protein/peptide bioconjugates. Due to the high amount of publications in this field, it highlights recent examples derived from the use of recent polymerization techniques such as controlled/living radical polymerization (CLRP). The covalent coupling between synthetic polymers and biological entities represents the main strategy with many different well-established coupling methods using native or unnatural functional groups that are available to construct desired bioconjugates. The corresponding reaction between an alkoxyamine and an aldehyde/ketone results in the formation of a stable oxime bond. Different strategies have been implemented so far to achieve polymer-protein/peptide bioconjugates via a non-covalent linkage. The apoenzyme/cofactor reconstitution strategy represents an interesting alternative to the construction of non-covalent polymer-protein bioconjugates. The covalent attachment of initiators can be compatible with not only the grafting from approach but also the non-covalent biotin/streptavidin (SAv) ligation methodology.

Original languageEnglish
Title of host publicationChemistry of Organo-Hybrids:
Subtitle of host publication Synthesis and Characterization of Functional Nano-Objects
EditorsBernadette Charleux, Christophe Coperet, Emmanuel Lacote
Place of PublicationNew Jersey USA
PublisherJohn Wiley & Sons
Chapter12
Pages466-502
Number of pages37
Edition1
ISBN (Electronic)9781118870068
ISBN (Print)9781118379028
DOIs
Publication statusPublished - 2015
Externally publishedYes

Keywords

  • Biotin/streptavidin (SAv) ligation methodology
  • Controlled/living radical polymerization (CLRP)
  • Covalent coupling
  • Polymer-protein/peptide bioconjugates
  • Synthetic polymers

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