Polyadenylation state microarray (PASTA) analysis

Traude Beilharz, Thomas Preiss

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Other

4 Citations (Scopus)

Abstract

Nearly all eukaryotic mRNAs terminate in a poly(A) tail that serves important roles in mRNA utilization. In the cytoplasm, the poly(A) tail promotes both mRNA stability and translation, and these functions are frequently regulated through changes in tail length. To identify the scope of poly(A) tail length control in a transcriptome, we developed the polyadenylation state microarray (PASTA) method. It involves the purification of mRNA based on poly(A) tail length using thermal elution from poly(U) sepharose, followed by microarray analysis of the resulting fractions. In this chapter we detail our PASTA approach and describe some methods for bulk and mRNA-specific poly(A) tail length measurements of use to monitor the procedure and independently verify the microarray data.
Original languageEnglish
Title of host publicationYeast Systems Biology: Methods and Protocols
EditorsJuan I Castrillo, Stephen G Oliver
Place of PublicationUSA
PublisherHumana Press
Pages133 - 147
Number of pages15
Edition1st
ISBN (Print)9781617791727
DOIs
Publication statusPublished - 2011

Cite this