PNA-DNA hybridization at the air-water interface in the presence of octadecylamine Langmuir monolayers

Vidya Ramakrishnan, Monnesha D'costa, Krishna N. Ganesh, Murali Sastry

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19 Citations (Scopus)

Abstract

Sequential immobilization of single-stranded complementary DNA and peptide nucleic acid (PNA) molecules at the air-water interface with cationic Langmuir monolayers is demonstrated. The complexation of the single-stranded DNA/PNA molecules with octadecylamine (ODA) Langmuir monolayers was followed in time by monitoring the pressure-area isotherms. A large (and slow) expansion of the ODA monolayer was observed during immobilization of single-stranded DNA at the air-water interface, and interestingly, no further expansion was detected on addition of PNA to the subphase. However, quartz crystal microgravimetry and UV-melting analysis of Langmuir-Blodgett films of the ODA-PNA-DNA hybrid monolayer clearly showed that the sequential immobilization process had resulted in hybridization of the complementary single-stranded DNA molecules with PNA at the air-water interface.

Original languageEnglish
Pages (from-to)6307-6311
Number of pages5
JournalLangmuir
Volume18
Issue number16
DOIs
Publication statusPublished - 6 Aug 2002
Externally publishedYes

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