Abstract
Sequential immobilization of single-stranded complementary DNA and peptide nucleic acid (PNA) molecules at the air-water interface with cationic Langmuir monolayers is demonstrated. The complexation of the single-stranded DNA/PNA molecules with octadecylamine (ODA) Langmuir monolayers was followed in time by monitoring the pressure-area isotherms. A large (and slow) expansion of the ODA monolayer was observed during immobilization of single-stranded DNA at the air-water interface, and interestingly, no further expansion was detected on addition of PNA to the subphase. However, quartz crystal microgravimetry and UV-melting analysis of Langmuir-Blodgett films of the ODA-PNA-DNA hybrid monolayer clearly showed that the sequential immobilization process had resulted in hybridization of the complementary single-stranded DNA molecules with PNA at the air-water interface.
Original language | English |
---|---|
Pages (from-to) | 6307-6311 |
Number of pages | 5 |
Journal | Langmuir |
Volume | 18 |
Issue number | 16 |
DOIs | |
Publication status | Published - 6 Aug 2002 |
Externally published | Yes |