Platelet receptor shedding

Elizabeth Gardiner, Mohammad Al-Tamimi, Robert Andrews, Michael Berndt

Research output: Contribution to journalArticleResearchpeer-review

19 Citations (Scopus)

Abstract

Receptor shedding is a mechanism for irreversible removal of transmembrane cell surface receptors by proteolysis of the receptor at a position near the extracellular surface of the plasma membrane. This process generates a soluble ectodomain fragment and a membrane-associated remnant fragment, and is distinct from loss of receptor surface expression by internalization or microparticle release or secretion of alternatively spliced soluble forms of receptors lacking a transmembrane domain. There has been an increased focus on new methods for analyzing shedding of platelet glycoprotein (GP)Ib-IX-V and GPVI because these receptors are platelet specific and are critical for the initiation of platelet adhesion and activation in thrombus formation at arterial shear rates. Platelet receptor shedding provides a mechanism for downregulating surface expression resulting in loss of ligand binding, decreasing the surface density affecting receptor cross linking and signalling and generation of proteolytic fragments that may be functional and/or provide platelet-specific biomarkers. A? 2012 Springer Science+Business Media, LLC.
Original languageEnglish
Pages (from-to)321 - 339
Number of pages19
JournalMethods in Molecular Biology
Volume788
DOIs
Publication statusPublished - 2012

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