TY - JOUR
T1 - Platelet apoptosis by cold-induced glycoprotein IbI? clustering
AU - van der Wal, Evertdina
AU - Du, Vivian X
AU - Lo, Kimberly
AU - Rasmussen, Jan Trige
AU - Verhoef, Sandra
AU - Akkerman, Jan-Willem N
PY - 2010
Y1 - 2010
N2 - Cold-storage of platelets followed by rewarming induces changes in Glycoprotein (GP) IbI?-distribution indicative of receptor clustering and initiates thromboxane A 2-formation. GPIbI? is associated with 14-3-3 proteins, which contribute to GPIbI?-signaling and in nucleated cells take part in apoptosis regulation.Objectives and methods: We investigated whether GPIbI?-clustering induces platelet apoptosis through 14-3-3 proteins during cold (4 h 0 A?C)-rewarming (1 h 37 A?C).Results: During cold-rewarming, 14-3-3 proteins associate with GPIbI? and dissociate from Bad inducing Bad-dephosphorylation and activation. This initiates pro-apoptosis changes in Bax/Bcl-x L and Bax-translocation to the mitochondria, inducing cytochrome c release. The result is activation of caspase-9, which triggers phosphatidylserine exposure and platelet phagocytosis by macrophages. Responses are prevented by N-acetyl-d-glucosamine (GN), which blocks GPIbI?-clustering, and by O-sialoglycoprotein endopeptidase, which removes extracellular GPIbI?.Conclusions: Cold-rewarming triggers apoptosis through a GN-sensitive GPIbI?-change indicative of receptor clustering. Attempts to improve platelet transfusion by cold-storage should focus on prevention of the GPIbI?-change. A? 2010 International Society on Thrombosis and Haemostasis.
AB - Cold-storage of platelets followed by rewarming induces changes in Glycoprotein (GP) IbI?-distribution indicative of receptor clustering and initiates thromboxane A 2-formation. GPIbI? is associated with 14-3-3 proteins, which contribute to GPIbI?-signaling and in nucleated cells take part in apoptosis regulation.Objectives and methods: We investigated whether GPIbI?-clustering induces platelet apoptosis through 14-3-3 proteins during cold (4 h 0 A?C)-rewarming (1 h 37 A?C).Results: During cold-rewarming, 14-3-3 proteins associate with GPIbI? and dissociate from Bad inducing Bad-dephosphorylation and activation. This initiates pro-apoptosis changes in Bax/Bcl-x L and Bax-translocation to the mitochondria, inducing cytochrome c release. The result is activation of caspase-9, which triggers phosphatidylserine exposure and platelet phagocytosis by macrophages. Responses are prevented by N-acetyl-d-glucosamine (GN), which blocks GPIbI?-clustering, and by O-sialoglycoprotein endopeptidase, which removes extracellular GPIbI?.Conclusions: Cold-rewarming triggers apoptosis through a GN-sensitive GPIbI?-change indicative of receptor clustering. Attempts to improve platelet transfusion by cold-storage should focus on prevention of the GPIbI?-change. A? 2010 International Society on Thrombosis and Haemostasis.
UR - http://onlinelibrary.wiley.com.ezproxy.lib.monash.edu.au/doi/10.1111/j.1538-7836.2010.04043.x/abstract?systemMessage=Wiley+Online+Library+will+be+disr
U2 - 10.1111/j.1538-7836.2010.04043.x
DO - 10.1111/j.1538-7836.2010.04043.x
M3 - Article
SN - 1538-7933
VL - 8
SP - 2554
EP - 2562
JO - Journal of Thrombosis and Haemostasis
JF - Journal of Thrombosis and Haemostasis
IS - 11
ER -