Plastic responses to four environmental stresses and cross-resistance in a laboratory population of Drosophila melanogaster

O Bubliy, Torsten Kristensen, Vanessa Kellermann, Volker Loeschcke

Research output: Contribution to journalArticleResearchpeer-review

Abstract

1. Acclimation or hardening to one stress in arthropods can lead to a plastic response, which confers increased resistance to other stresses. Such cross-resistance may indicate shared physiological resistance mechanisms and a possibility of joint evolution for resistance traits. 2. In this study, we tested for cross-resistance using Drosophila melanogaster as a model system. Adult females were assayed for resistance to cold, heat, desiccation and starvation following cold acclimation, heat hardening, desiccation hardening and starvation acclimation, resulting in 12 pretreatment-by-test combinations for estimating potential cross-resistance effects. The acclimation / hardening regimes were chosen so that flies enhanced their resistance to the same type of stress as that used for the particular pretreatment. 3. Two cases of cross-resistance were found, with desiccation-hardened and starvation-acclimated flies being more resistant than control ones in heat and desiccation resistance tests, respectively. In four cases, no acclimation / hardening effect was observed, and for six pretreatment- by-test combinations, the effect of acclimation / hardening was negative. 4. We also revealed that heat and desiccation hardening as well as acclimation to starvation had a cost under non-stressful conditions leading to reduced longevity. Cold acclimation did not affect longevity, although its effect was difficult to estimate precisely: during pretreatment at a low temperature, biological ageing of the flies might be delayed. 5. The pattern of acclimation / hardening responses considered in the context of literature data on stress resistance indicates that expression of heat-shock proteins is not likely to contribute to the observed cross-resistance, but other probable general stress resistance mechanisms such as reduction of metabolic rate and accumulation of energy reserves might be involved. 6. The lack of cross-resistance induced by acclimation / hardening treatments suggests that in an environment with multiple stresses, evolution of shared protective systems associated with plastic responses may be constrained.
Original languageEnglish
Pages (from-to)245 - 253
Number of pages9
JournalFunctional Ecology
Volume26
Issue number1
DOIs
Publication statusPublished - 2012
Externally publishedYes

Cite this

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title = "Plastic responses to four environmental stresses and cross-resistance in a laboratory population of Drosophila melanogaster",
abstract = "1. Acclimation or hardening to one stress in arthropods can lead to a plastic response, which confers increased resistance to other stresses. Such cross-resistance may indicate shared physiological resistance mechanisms and a possibility of joint evolution for resistance traits. 2. In this study, we tested for cross-resistance using Drosophila melanogaster as a model system. Adult females were assayed for resistance to cold, heat, desiccation and starvation following cold acclimation, heat hardening, desiccation hardening and starvation acclimation, resulting in 12 pretreatment-by-test combinations for estimating potential cross-resistance effects. The acclimation / hardening regimes were chosen so that flies enhanced their resistance to the same type of stress as that used for the particular pretreatment. 3. Two cases of cross-resistance were found, with desiccation-hardened and starvation-acclimated flies being more resistant than control ones in heat and desiccation resistance tests, respectively. In four cases, no acclimation / hardening effect was observed, and for six pretreatment- by-test combinations, the effect of acclimation / hardening was negative. 4. We also revealed that heat and desiccation hardening as well as acclimation to starvation had a cost under non-stressful conditions leading to reduced longevity. Cold acclimation did not affect longevity, although its effect was difficult to estimate precisely: during pretreatment at a low temperature, biological ageing of the flies might be delayed. 5. The pattern of acclimation / hardening responses considered in the context of literature data on stress resistance indicates that expression of heat-shock proteins is not likely to contribute to the observed cross-resistance, but other probable general stress resistance mechanisms such as reduction of metabolic rate and accumulation of energy reserves might be involved. 6. The lack of cross-resistance induced by acclimation / hardening treatments suggests that in an environment with multiple stresses, evolution of shared protective systems associated with plastic responses may be constrained.",
author = "O Bubliy and Torsten Kristensen and Vanessa Kellermann and Volker Loeschcke",
year = "2012",
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journal = "Functional Ecology",
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Plastic responses to four environmental stresses and cross-resistance in a laboratory population of Drosophila melanogaster. / Bubliy, O; Kristensen, Torsten; Kellermann, Vanessa; Loeschcke, Volker.

In: Functional Ecology, Vol. 26, No. 1, 2012, p. 245 - 253.

Research output: Contribution to journalArticleResearchpeer-review

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T1 - Plastic responses to four environmental stresses and cross-resistance in a laboratory population of Drosophila melanogaster

AU - Bubliy, O

AU - Kristensen, Torsten

AU - Kellermann, Vanessa

AU - Loeschcke, Volker

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N2 - 1. Acclimation or hardening to one stress in arthropods can lead to a plastic response, which confers increased resistance to other stresses. Such cross-resistance may indicate shared physiological resistance mechanisms and a possibility of joint evolution for resistance traits. 2. In this study, we tested for cross-resistance using Drosophila melanogaster as a model system. Adult females were assayed for resistance to cold, heat, desiccation and starvation following cold acclimation, heat hardening, desiccation hardening and starvation acclimation, resulting in 12 pretreatment-by-test combinations for estimating potential cross-resistance effects. The acclimation / hardening regimes were chosen so that flies enhanced their resistance to the same type of stress as that used for the particular pretreatment. 3. Two cases of cross-resistance were found, with desiccation-hardened and starvation-acclimated flies being more resistant than control ones in heat and desiccation resistance tests, respectively. In four cases, no acclimation / hardening effect was observed, and for six pretreatment- by-test combinations, the effect of acclimation / hardening was negative. 4. We also revealed that heat and desiccation hardening as well as acclimation to starvation had a cost under non-stressful conditions leading to reduced longevity. Cold acclimation did not affect longevity, although its effect was difficult to estimate precisely: during pretreatment at a low temperature, biological ageing of the flies might be delayed. 5. The pattern of acclimation / hardening responses considered in the context of literature data on stress resistance indicates that expression of heat-shock proteins is not likely to contribute to the observed cross-resistance, but other probable general stress resistance mechanisms such as reduction of metabolic rate and accumulation of energy reserves might be involved. 6. The lack of cross-resistance induced by acclimation / hardening treatments suggests that in an environment with multiple stresses, evolution of shared protective systems associated with plastic responses may be constrained.

AB - 1. Acclimation or hardening to one stress in arthropods can lead to a plastic response, which confers increased resistance to other stresses. Such cross-resistance may indicate shared physiological resistance mechanisms and a possibility of joint evolution for resistance traits. 2. In this study, we tested for cross-resistance using Drosophila melanogaster as a model system. Adult females were assayed for resistance to cold, heat, desiccation and starvation following cold acclimation, heat hardening, desiccation hardening and starvation acclimation, resulting in 12 pretreatment-by-test combinations for estimating potential cross-resistance effects. The acclimation / hardening regimes were chosen so that flies enhanced their resistance to the same type of stress as that used for the particular pretreatment. 3. Two cases of cross-resistance were found, with desiccation-hardened and starvation-acclimated flies being more resistant than control ones in heat and desiccation resistance tests, respectively. In four cases, no acclimation / hardening effect was observed, and for six pretreatment- by-test combinations, the effect of acclimation / hardening was negative. 4. We also revealed that heat and desiccation hardening as well as acclimation to starvation had a cost under non-stressful conditions leading to reduced longevity. Cold acclimation did not affect longevity, although its effect was difficult to estimate precisely: during pretreatment at a low temperature, biological ageing of the flies might be delayed. 5. The pattern of acclimation / hardening responses considered in the context of literature data on stress resistance indicates that expression of heat-shock proteins is not likely to contribute to the observed cross-resistance, but other probable general stress resistance mechanisms such as reduction of metabolic rate and accumulation of energy reserves might be involved. 6. The lack of cross-resistance induced by acclimation / hardening treatments suggests that in an environment with multiple stresses, evolution of shared protective systems associated with plastic responses may be constrained.

UR - http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2435.2011.01928.x/pdf

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DO - 10.1111/j.1365-2435.2011.01928.x

M3 - Article

VL - 26

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EP - 253

JO - Functional Ecology

JF - Functional Ecology

SN - 0269-8463

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