Plasmodium falciparum: Comparative analysis of erythrocyte stage-dependent protein antigens

Graham V. Brown; Ross L. Coppel; Helena Vrbova; Raelene J. Grumont; Robin F. Anders

doi:10.1016/0014-4894(82)90070-4

Plasmodium falciparum: Comparative analysis of erythrocyte stage-dependent protein antigens

Graham V. Brown, Ross L. Coppel, Helena Vrbova, Raelene J. Grumont, Robin F. Anders

Research output: Contribution to journal › Article › Research › peer-review

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Abstract

Proteins of erythrocytic stages of Plasmodium falciparum were biosynthetically labeled at different times during the first cycle of in vitro synchronous cultivation after collection from patients in the Madang region of Papua New Guinea. Proteins were immunoprecipitated with a pool of hyperimmune serum collected in the region then analyzed by sodium dodecyl sulfate-gel electrophoresis. Antigens were recognized in all life cycle stages but the majority of antigens, particularly those of high molecular weight, were present in the mature forms of the parasite.

Original language	English
Pages (from-to)	279-284
Number of pages	6
Journal	Experimental Parasitology
Volume	53
Issue number	2
DOIs	https://doi.org/10.1016/0014-4894(82)90070-4
Publication status	Published - 1 Jan 1982
Externally published	Yes

Keywords

Antigens
Biosynthetic labeling
Blood stages
Cultivation
Hyperimmune sera, human
Malaria, human
Plasmodium falciparum
Protozoa, parasitic

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/0014-4894(82)90070-4

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title = "Plasmodium falciparum: Comparative analysis of erythrocyte stage-dependent protein antigens",

abstract = "Proteins of erythrocytic stages of Plasmodium falciparum were biosynthetically labeled at different times during the first cycle of in vitro synchronous cultivation after collection from patients in the Madang region of Papua New Guinea. Proteins were immunoprecipitated with a pool of hyperimmune serum collected in the region then analyzed by sodium dodecyl sulfate-gel electrophoresis. Antigens were recognized in all life cycle stages but the majority of antigens, particularly those of high molecular weight, were present in the mature forms of the parasite.",

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T1 - Plasmodium falciparum

T2 - Comparative analysis of erythrocyte stage-dependent protein antigens

AU - Brown, Graham V.

AU - Coppel, Ross L.

AU - Vrbova, Helena

AU - Grumont, Raelene J.

AU - Anders, Robin F.

PY - 1982/1/1

Y1 - 1982/1/1

N2 - Proteins of erythrocytic stages of Plasmodium falciparum were biosynthetically labeled at different times during the first cycle of in vitro synchronous cultivation after collection from patients in the Madang region of Papua New Guinea. Proteins were immunoprecipitated with a pool of hyperimmune serum collected in the region then analyzed by sodium dodecyl sulfate-gel electrophoresis. Antigens were recognized in all life cycle stages but the majority of antigens, particularly those of high molecular weight, were present in the mature forms of the parasite.

AB - Proteins of erythrocytic stages of Plasmodium falciparum were biosynthetically labeled at different times during the first cycle of in vitro synchronous cultivation after collection from patients in the Madang region of Papua New Guinea. Proteins were immunoprecipitated with a pool of hyperimmune serum collected in the region then analyzed by sodium dodecyl sulfate-gel electrophoresis. Antigens were recognized in all life cycle stages but the majority of antigens, particularly those of high molecular weight, were present in the mature forms of the parasite.

KW - Antigens

KW - Biosynthetic labeling

KW - Blood stages

KW - Cultivation

KW - Hyperimmune sera, human

KW - Malaria, human

KW - Plasmodium falciparum

KW - Protozoa, parasitic

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AN - SCOPUS:0019953535

SN - 0014-4894

VL - 53

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EP - 284

JO - Experimental Parasitology

JF - Experimental Parasitology

IS - 2

ER -

Plasmodium falciparum: Comparative analysis of erythrocyte stage-dependent protein antigens

Abstract

Keywords

UN SDGs

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