Plasma polymer and PEG-based coatings for DNA, protein and cell microarrays

Andrew L. Hook, Nicolas Voelcker, Helmut Thissen

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Researchpeer-review

3 Citations (Scopus)

Abstract

DNA, protein and cell microarrays are increasingly used in a multitude of bioassays. All of these arrays require substrates that are suitable for the immobilisation and display of arrayed probe molecules whilst at the same time resisting non-specific interactions of biomolecules with the substrate in areas between printed spots. To meet these conflicting requirements, three different approaches have been developed, all of which were based on low-fouling, high-density poly(ethylene glycol) (PEG) background coatings. In the first approach, the coating was based on allylamine plasma polymerisation (ALAPP) and the subsequent high-density grafting of PEG, followed by the generation of a surface chemical pattern using laser ablation. In the second approach, a photoreactive polymer was printed on the same ALAPP-PEG background. The third approach was based on ALAPP deposition followed by the formation of a multifunctional layer by spin coating a PEG-based polymer that also displayed epoxy groups. The successful demonstration of DNA, protein and cell microarrays has been achieved on each of these coatings.

Original languageEnglish
Title of host publicationCell-Based Microarrays
Subtitle of host publicationMethods and Protocols
EditorsElla Palmer
Place of PublicationNew York NY USA
PublisherHumana Press
Chapter13
Pages159-170
Number of pages12
ISBN (Electronic)9781617379703
ISBN (Print)9781617379697
DOIs
Publication statusPublished - 2011
Externally publishedYes

Publication series

NameMethods in Molecular Biology
PublisherHumana Press
Volume706
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Patterned substrates
  • plasma polymerisation
  • PEG
  • laser ablation
  • contact printing
  • inkjet printing
  • microarrays

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