Plasma advanced glycation end products (AGEs) and NF-(kappa)B activity are independent determinants of diastolic and pulse pressure

Karly Sourris, Jasmine Lyons, Sonia L Dougherty, Vibhasha Chand, Nora Straznicky, Markus P Schlaich, Mariee Grima, Mark Emmanuel Cooper, Bronwyn A Kingwell, Maximilian Pangratius J De Courten, Josephine M Forbes, Barbora de Courten

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Background: High levels of circulating advanced glycation end products (AGEs) can initiate chronic low-grade activation of the immune system (CLAIS) with each of these factors independently associated with cardiovascular (CV) morbidity and mortality. Therefore, our objective was to characterize the relationship between serum AGEs, CLAIS and other risk factors for CV disease in normotensive non-diabetic individuals. Methods: We measured body mass index (BMI), waistto-hip ratio (WHR), blood pressure, lipid and glucose profile in 44 non-diabetic volunteers (17 female, 27 males). Carboxymethyl-lysine (CML) was measured by ELISA as a marker for circulating AGEs and NF-?B p65 activity as an inflammatory marker by DNA-binding in peripheral blood mononuclear cells lysates (PBMC). Results: Plasma CML concentrations were related to diastolic blood pressure (r = .0.51, p <0.01) independently of age, sex, BMI and WHR (p <0.05). Diastolic blood pressure was also related to NF-eB activity in PBMC (r = 0.47, p <0.01) before and after adjustment for age, sex, BMI and WHR (p <0.05). Plasma CML concentrations were related to the pulse pressure before (r = 0.42; p <0.05) and after adjustment for age, sex, BMI and waist (p <0.05). Neither CML nor NF-?B activity were related to systolic blood pressure (both p = ns). Plasma CML concentrations were not associated with plasma lipid or glucose concentrations (all p = ns). Conclusions: Plasma AGE levels and NF-eB activity in PBMC were independent determinants of diastolic and pulse pressure in healthy normotensive individuals. This association suggests a role for AGEs in the etiology of hypertension, possibly via the initiation of CLAIS and aortic stiffening.
Original languageEnglish
Pages (from-to)129 - 138
Number of pages10
JournalClinical Chemistry and Laboratory Medicine
Volume52
Issue number1
DOIs
Publication statusPublished - 2014

Cite this

@article{45014502ce8f43749bfd1bad753520c1,
title = "Plasma advanced glycation end products (AGEs) and NF-(kappa)B activity are independent determinants of diastolic and pulse pressure",
abstract = "Background: High levels of circulating advanced glycation end products (AGEs) can initiate chronic low-grade activation of the immune system (CLAIS) with each of these factors independently associated with cardiovascular (CV) morbidity and mortality. Therefore, our objective was to characterize the relationship between serum AGEs, CLAIS and other risk factors for CV disease in normotensive non-diabetic individuals. Methods: We measured body mass index (BMI), waistto-hip ratio (WHR), blood pressure, lipid and glucose profile in 44 non-diabetic volunteers (17 female, 27 males). Carboxymethyl-lysine (CML) was measured by ELISA as a marker for circulating AGEs and NF-?B p65 activity as an inflammatory marker by DNA-binding in peripheral blood mononuclear cells lysates (PBMC). Results: Plasma CML concentrations were related to diastolic blood pressure (r = .0.51, p <0.01) independently of age, sex, BMI and WHR (p <0.05). Diastolic blood pressure was also related to NF-eB activity in PBMC (r = 0.47, p <0.01) before and after adjustment for age, sex, BMI and WHR (p <0.05). Plasma CML concentrations were related to the pulse pressure before (r = 0.42; p <0.05) and after adjustment for age, sex, BMI and waist (p <0.05). Neither CML nor NF-?B activity were related to systolic blood pressure (both p = ns). Plasma CML concentrations were not associated with plasma lipid or glucose concentrations (all p = ns). Conclusions: Plasma AGE levels and NF-eB activity in PBMC were independent determinants of diastolic and pulse pressure in healthy normotensive individuals. This association suggests a role for AGEs in the etiology of hypertension, possibly via the initiation of CLAIS and aortic stiffening.",
author = "Karly Sourris and Jasmine Lyons and Dougherty, {Sonia L} and Vibhasha Chand and Nora Straznicky and Schlaich, {Markus P} and Mariee Grima and Cooper, {Mark Emmanuel} and Kingwell, {Bronwyn A} and {De Courten}, {Maximilian Pangratius J} and Forbes, {Josephine M} and {de Courten}, Barbora",
year = "2014",
doi = "10.1515/cclm-2012-0850",
language = "English",
volume = "52",
pages = "129 -- 138",
journal = "Clinical Chemistry and Laboratory Medicine",
issn = "1434-6621",
publisher = "Walter de Gruyter",
number = "1",

}

Plasma advanced glycation end products (AGEs) and NF-(kappa)B activity are independent determinants of diastolic and pulse pressure. / Sourris, Karly; Lyons, Jasmine; Dougherty, Sonia L; Chand, Vibhasha; Straznicky, Nora; Schlaich, Markus P; Grima, Mariee; Cooper, Mark Emmanuel; Kingwell, Bronwyn A; De Courten, Maximilian Pangratius J; Forbes, Josephine M; de Courten, Barbora.

In: Clinical Chemistry and Laboratory Medicine, Vol. 52, No. 1, 2014, p. 129 - 138.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Plasma advanced glycation end products (AGEs) and NF-(kappa)B activity are independent determinants of diastolic and pulse pressure

AU - Sourris, Karly

AU - Lyons, Jasmine

AU - Dougherty, Sonia L

AU - Chand, Vibhasha

AU - Straznicky, Nora

AU - Schlaich, Markus P

AU - Grima, Mariee

AU - Cooper, Mark Emmanuel

AU - Kingwell, Bronwyn A

AU - De Courten, Maximilian Pangratius J

AU - Forbes, Josephine M

AU - de Courten, Barbora

PY - 2014

Y1 - 2014

N2 - Background: High levels of circulating advanced glycation end products (AGEs) can initiate chronic low-grade activation of the immune system (CLAIS) with each of these factors independently associated with cardiovascular (CV) morbidity and mortality. Therefore, our objective was to characterize the relationship between serum AGEs, CLAIS and other risk factors for CV disease in normotensive non-diabetic individuals. Methods: We measured body mass index (BMI), waistto-hip ratio (WHR), blood pressure, lipid and glucose profile in 44 non-diabetic volunteers (17 female, 27 males). Carboxymethyl-lysine (CML) was measured by ELISA as a marker for circulating AGEs and NF-?B p65 activity as an inflammatory marker by DNA-binding in peripheral blood mononuclear cells lysates (PBMC). Results: Plasma CML concentrations were related to diastolic blood pressure (r = .0.51, p <0.01) independently of age, sex, BMI and WHR (p <0.05). Diastolic blood pressure was also related to NF-eB activity in PBMC (r = 0.47, p <0.01) before and after adjustment for age, sex, BMI and WHR (p <0.05). Plasma CML concentrations were related to the pulse pressure before (r = 0.42; p <0.05) and after adjustment for age, sex, BMI and waist (p <0.05). Neither CML nor NF-?B activity were related to systolic blood pressure (both p = ns). Plasma CML concentrations were not associated with plasma lipid or glucose concentrations (all p = ns). Conclusions: Plasma AGE levels and NF-eB activity in PBMC were independent determinants of diastolic and pulse pressure in healthy normotensive individuals. This association suggests a role for AGEs in the etiology of hypertension, possibly via the initiation of CLAIS and aortic stiffening.

AB - Background: High levels of circulating advanced glycation end products (AGEs) can initiate chronic low-grade activation of the immune system (CLAIS) with each of these factors independently associated with cardiovascular (CV) morbidity and mortality. Therefore, our objective was to characterize the relationship between serum AGEs, CLAIS and other risk factors for CV disease in normotensive non-diabetic individuals. Methods: We measured body mass index (BMI), waistto-hip ratio (WHR), blood pressure, lipid and glucose profile in 44 non-diabetic volunteers (17 female, 27 males). Carboxymethyl-lysine (CML) was measured by ELISA as a marker for circulating AGEs and NF-?B p65 activity as an inflammatory marker by DNA-binding in peripheral blood mononuclear cells lysates (PBMC). Results: Plasma CML concentrations were related to diastolic blood pressure (r = .0.51, p <0.01) independently of age, sex, BMI and WHR (p <0.05). Diastolic blood pressure was also related to NF-eB activity in PBMC (r = 0.47, p <0.01) before and after adjustment for age, sex, BMI and WHR (p <0.05). Plasma CML concentrations were related to the pulse pressure before (r = 0.42; p <0.05) and after adjustment for age, sex, BMI and waist (p <0.05). Neither CML nor NF-?B activity were related to systolic blood pressure (both p = ns). Plasma CML concentrations were not associated with plasma lipid or glucose concentrations (all p = ns). Conclusions: Plasma AGE levels and NF-eB activity in PBMC were independent determinants of diastolic and pulse pressure in healthy normotensive individuals. This association suggests a role for AGEs in the etiology of hypertension, possibly via the initiation of CLAIS and aortic stiffening.

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U2 - 10.1515/cclm-2012-0850

DO - 10.1515/cclm-2012-0850

M3 - Article

VL - 52

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EP - 138

JO - Clinical Chemistry and Laboratory Medicine

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SN - 1434-6621

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