Placental restriction alters the functional development of the pituitary-adrenal axis in the sheep fetus during late gestation

Ian D. Phillips, Giuseppe Simonetta, Julie A. Owens, Jeffrey S. Robinson, Iain J. Clarke, I. Caroline McMillen

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Abstract

We have experimentally restricted placental growth in the sheep to investigate the impact of reduced substrate delivery on fetal pituitary proopiomelanocortin (POMC) mRNA levels and on circulating ACTH 1-39, immunoreactive ACTH, and cortisol concentrations during late gestation. Endometrial caruncles were removed in nine ewes before mating to reduce the number of placentomes formed [placental restriction group (PR)]. Fetal arterial PO2 and O2 saturation were reduced in the PR group (2.0 ± 0.1 kPa and 42.8 ± 1.1%, n = 9) when compared with control fetuses (3.1 ± 0.1 kPa and 66.4 ± 0.9%, n = 10). The ratio of anterior pituitary POMC mRNA:18 S ribosomal RNA was also lower (p < 0.05) in the PR group (0.49 ± 0.05) when compared with the control group (0.80 ± 0.12) after 140 d of gestation. In contrast, plasma concentrations of ACTH 1-39 and immunoreactive ACTH were similar in the PR and control groups throughout late gestation. Plasma ACTH 1-39 concentrations increased (p < 0.0116) between 128 and 134 d of gestation, in both the PR (122-128 d: 2.70 ± 0.34 pmol/L: 134-141 d; 7.07 ± 1.57 pmol/L) and control (122-128 d; 3.36 ± 0.56 pmol/L: 134-141 d; 10.78 ± 2.88 pmol/L) groups. Combined adrenal weight was higher (p < 0.005) in the PR group (130 ± 10 mg/kg) compared with controls (80 ± 1 mg/kg) at 140 d of gestation, and plasma cortisol concentrations were also higher (p < 0.02) in PR than control fetuses between 127 and 141 d of gestation. These changes imply that the fetal hypothalamo-pituitary-adrenal axis is operating at a new central set point in the growth-restricted fetus.

Original languageEnglish
Pages (from-to)861-866
Number of pages6
JournalPediatric Research
Volume40
Issue number6
DOIs
Publication statusPublished - 1 Jan 1996
Externally publishedYes

Cite this

Phillips, Ian D. ; Simonetta, Giuseppe ; Owens, Julie A. ; Robinson, Jeffrey S. ; Clarke, Iain J. ; McMillen, I. Caroline. / Placental restriction alters the functional development of the pituitary-adrenal axis in the sheep fetus during late gestation. In: Pediatric Research. 1996 ; Vol. 40, No. 6. pp. 861-866.
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abstract = "We have experimentally restricted placental growth in the sheep to investigate the impact of reduced substrate delivery on fetal pituitary proopiomelanocortin (POMC) mRNA levels and on circulating ACTH 1-39, immunoreactive ACTH, and cortisol concentrations during late gestation. Endometrial caruncles were removed in nine ewes before mating to reduce the number of placentomes formed [placental restriction group (PR)]. Fetal arterial PO2 and O2 saturation were reduced in the PR group (2.0 ± 0.1 kPa and 42.8 ± 1.1{\%}, n = 9) when compared with control fetuses (3.1 ± 0.1 kPa and 66.4 ± 0.9{\%}, n = 10). The ratio of anterior pituitary POMC mRNA:18 S ribosomal RNA was also lower (p < 0.05) in the PR group (0.49 ± 0.05) when compared with the control group (0.80 ± 0.12) after 140 d of gestation. In contrast, plasma concentrations of ACTH 1-39 and immunoreactive ACTH were similar in the PR and control groups throughout late gestation. Plasma ACTH 1-39 concentrations increased (p < 0.0116) between 128 and 134 d of gestation, in both the PR (122-128 d: 2.70 ± 0.34 pmol/L: 134-141 d; 7.07 ± 1.57 pmol/L) and control (122-128 d; 3.36 ± 0.56 pmol/L: 134-141 d; 10.78 ± 2.88 pmol/L) groups. Combined adrenal weight was higher (p < 0.005) in the PR group (130 ± 10 mg/kg) compared with controls (80 ± 1 mg/kg) at 140 d of gestation, and plasma cortisol concentrations were also higher (p < 0.02) in PR than control fetuses between 127 and 141 d of gestation. These changes imply that the fetal hypothalamo-pituitary-adrenal axis is operating at a new central set point in the growth-restricted fetus.",
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Placental restriction alters the functional development of the pituitary-adrenal axis in the sheep fetus during late gestation. / Phillips, Ian D.; Simonetta, Giuseppe; Owens, Julie A.; Robinson, Jeffrey S.; Clarke, Iain J.; McMillen, I. Caroline.

In: Pediatric Research, Vol. 40, No. 6, 01.01.1996, p. 861-866.

Research output: Contribution to journalArticleResearchpeer-review

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T1 - Placental restriction alters the functional development of the pituitary-adrenal axis in the sheep fetus during late gestation

AU - Phillips, Ian D.

AU - Simonetta, Giuseppe

AU - Owens, Julie A.

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AB - We have experimentally restricted placental growth in the sheep to investigate the impact of reduced substrate delivery on fetal pituitary proopiomelanocortin (POMC) mRNA levels and on circulating ACTH 1-39, immunoreactive ACTH, and cortisol concentrations during late gestation. Endometrial caruncles were removed in nine ewes before mating to reduce the number of placentomes formed [placental restriction group (PR)]. Fetal arterial PO2 and O2 saturation were reduced in the PR group (2.0 ± 0.1 kPa and 42.8 ± 1.1%, n = 9) when compared with control fetuses (3.1 ± 0.1 kPa and 66.4 ± 0.9%, n = 10). The ratio of anterior pituitary POMC mRNA:18 S ribosomal RNA was also lower (p < 0.05) in the PR group (0.49 ± 0.05) when compared with the control group (0.80 ± 0.12) after 140 d of gestation. In contrast, plasma concentrations of ACTH 1-39 and immunoreactive ACTH were similar in the PR and control groups throughout late gestation. Plasma ACTH 1-39 concentrations increased (p < 0.0116) between 128 and 134 d of gestation, in both the PR (122-128 d: 2.70 ± 0.34 pmol/L: 134-141 d; 7.07 ± 1.57 pmol/L) and control (122-128 d; 3.36 ± 0.56 pmol/L: 134-141 d; 10.78 ± 2.88 pmol/L) groups. Combined adrenal weight was higher (p < 0.005) in the PR group (130 ± 10 mg/kg) compared with controls (80 ± 1 mg/kg) at 140 d of gestation, and plasma cortisol concentrations were also higher (p < 0.02) in PR than control fetuses between 127 and 141 d of gestation. These changes imply that the fetal hypothalamo-pituitary-adrenal axis is operating at a new central set point in the growth-restricted fetus.

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