Piwi and piRNAs Act Upstream of an Endogenous siRNA Pathway to Suppress Tc3 Transposon Mobility in the Caenorhabditis elegans Germline

Partha P. Das; Marloes P. Bagijn; Leonard D. Goldstein; Julie R. Woolford; Nicolas J. Lehrbach; Alexandra Sapetschnig; Heeran R. Buhecha; Michael J. Gilchrist; Kevin L. Howe; Rory Stark; Nik Matthews; Eugene Berezikov; René F. Ketting; Simon Tavaré; Eric A. Miska

doi:10.1016/j.molcel.2008.06.003

Piwi and piRNAs Act Upstream of an Endogenous siRNA Pathway to Suppress Tc3 Transposon Mobility in the Caenorhabditis elegans Germline

Partha P. Das, Marloes P. Bagijn, Leonard D. Goldstein, Julie R. Woolford, Nicolas J. Lehrbach, Alexandra Sapetschnig, Heeran R. Buhecha, Michael J. Gilchrist, Kevin L. Howe, Rory Stark, Nik Matthews, Eugene Berezikov, René F. Ketting, Simon Tavaré, Eric A. Miska

Research output: Contribution to journal › Article › Research › peer-review

240 Citations (Scopus)

Abstract

The Piwi proteins of the Argonaute superfamily are required for normal germline development in Drosophila, zebrafish, and mice and associate with 24-30 nucleotide RNAs termed piRNAs. We identify a class of 21 nucleotide RNAs, previously named 21U-RNAs, as the piRNAs of C. elegans. Piwi and piRNA expression is restricted to the male and female germline and independent of many proteins in other small-RNA pathways, including DCR-1. We show that Piwi is specifically required to silence Tc3, but not other Tc/mariner DNA transposons. Tc3 excision rates in the germline are increased at least 100-fold in piwi mutants as compared to wild-type. We find no evidence for a Ping-Pong model for piRNA amplification in C. elegans. Instead, we demonstrate that Piwi acts upstream of an endogenous siRNA pathway in Tc3 silencing. These data might suggest a link between piRNA and siRNA function.

Original language	English
Pages (from-to)	79-90
Number of pages	12
Journal	Molecular Cell
Volume	31
Issue number	1
DOIs	https://doi.org/10.1016/j.molcel.2008.06.003
Publication status	Published - 11 Jul 2008
Externally published	Yes

Keywords

DEVBIO
RNA

Access to Document

10.1016/j.molcel.2008.06.003

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Cite this

Das, P. P., Bagijn, M. P., Goldstein, L. D., Woolford, J. R., Lehrbach, N. J., Sapetschnig, A., Buhecha, H. R., Gilchrist, M. J., Howe, K. L., Stark, R., Matthews, N., Berezikov, E., Ketting, R. F., Tavaré, S., & Miska, E. A. (2008). Piwi and piRNAs Act Upstream of an Endogenous siRNA Pathway to Suppress Tc3 Transposon Mobility in the Caenorhabditis elegans Germline. Molecular Cell, 31(1), 79-90. https://doi.org/10.1016/j.molcel.2008.06.003

Das, Partha P. ; Bagijn, Marloes P. ; Goldstein, Leonard D. ; Woolford, Julie R. ; Lehrbach, Nicolas J. ; Sapetschnig, Alexandra ; Buhecha, Heeran R. ; Gilchrist, Michael J. ; Howe, Kevin L. ; Stark, Rory ; Matthews, Nik ; Berezikov, Eugene ; Ketting, René F. ; Tavaré, Simon ; Miska, Eric A. / Piwi and piRNAs Act Upstream of an Endogenous siRNA Pathway to Suppress Tc3 Transposon Mobility in the Caenorhabditis elegans Germline. In: Molecular Cell. 2008 ; Vol. 31, No. 1. pp. 79-90.

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title = "Piwi and piRNAs Act Upstream of an Endogenous siRNA Pathway to Suppress Tc3 Transposon Mobility in the Caenorhabditis elegans Germline",

abstract = "The Piwi proteins of the Argonaute superfamily are required for normal germline development in Drosophila, zebrafish, and mice and associate with 24-30 nucleotide RNAs termed piRNAs. We identify a class of 21 nucleotide RNAs, previously named 21U-RNAs, as the piRNAs of C. elegans. Piwi and piRNA expression is restricted to the male and female germline and independent of many proteins in other small-RNA pathways, including DCR-1. We show that Piwi is specifically required to silence Tc3, but not other Tc/mariner DNA transposons. Tc3 excision rates in the germline are increased at least 100-fold in piwi mutants as compared to wild-type. We find no evidence for a Ping-Pong model for piRNA amplification in C. elegans. Instead, we demonstrate that Piwi acts upstream of an endogenous siRNA pathway in Tc3 silencing. These data might suggest a link between piRNA and siRNA function.",

keywords = "DEVBIO, RNA",

author = "Das, {Partha P.} and Bagijn, {Marloes P.} and Goldstein, {Leonard D.} and Woolford, {Julie R.} and Lehrbach, {Nicolas J.} and Alexandra Sapetschnig and Buhecha, {Heeran R.} and Gilchrist, {Michael J.} and Howe, {Kevin L.} and Rory Stark and Nik Matthews and Eugene Berezikov and Ketting, {Ren{\'e} F.} and Simon Tavar{\'e} and Miska, {Eric A.}",

year = "2008",

month = jul,

day = "11",

doi = "10.1016/j.molcel.2008.06.003",

language = "English",

volume = "31",

pages = "79--90",

journal = "Molecular Cell",

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Das, PP, Bagijn, MP, Goldstein, LD, Woolford, JR, Lehrbach, NJ, Sapetschnig, A, Buhecha, HR, Gilchrist, MJ, Howe, KL, Stark, R, Matthews, N, Berezikov, E, Ketting, RF, Tavaré, S & Miska, EA 2008, 'Piwi and piRNAs Act Upstream of an Endogenous siRNA Pathway to Suppress Tc3 Transposon Mobility in the Caenorhabditis elegans Germline', Molecular Cell, vol. 31, no. 1, pp. 79-90. https://doi.org/10.1016/j.molcel.2008.06.003

Piwi and piRNAs Act Upstream of an Endogenous siRNA Pathway to Suppress Tc3 Transposon Mobility in the Caenorhabditis elegans Germline. / Das, Partha P.; Bagijn, Marloes P.; Goldstein, Leonard D.; Woolford, Julie R.; Lehrbach, Nicolas J.; Sapetschnig, Alexandra; Buhecha, Heeran R.; Gilchrist, Michael J.; Howe, Kevin L.; Stark, Rory; Matthews, Nik; Berezikov, Eugene; Ketting, René F.; Tavaré, Simon; Miska, Eric A.

In: Molecular Cell, Vol. 31, No. 1, 11.07.2008, p. 79-90.

Research output: Contribution to journal › Article › Research › peer-review

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T1 - Piwi and piRNAs Act Upstream of an Endogenous siRNA Pathway to Suppress Tc3 Transposon Mobility in the Caenorhabditis elegans Germline

AU - Das, Partha P.

AU - Bagijn, Marloes P.

AU - Goldstein, Leonard D.

AU - Woolford, Julie R.

AU - Lehrbach, Nicolas J.

AU - Sapetschnig, Alexandra

AU - Buhecha, Heeran R.

AU - Gilchrist, Michael J.

AU - Howe, Kevin L.

AU - Stark, Rory

AU - Matthews, Nik

AU - Berezikov, Eugene

AU - Ketting, René F.

AU - Tavaré, Simon

AU - Miska, Eric A.

PY - 2008/7/11

Y1 - 2008/7/11

N2 - The Piwi proteins of the Argonaute superfamily are required for normal germline development in Drosophila, zebrafish, and mice and associate with 24-30 nucleotide RNAs termed piRNAs. We identify a class of 21 nucleotide RNAs, previously named 21U-RNAs, as the piRNAs of C. elegans. Piwi and piRNA expression is restricted to the male and female germline and independent of many proteins in other small-RNA pathways, including DCR-1. We show that Piwi is specifically required to silence Tc3, but not other Tc/mariner DNA transposons. Tc3 excision rates in the germline are increased at least 100-fold in piwi mutants as compared to wild-type. We find no evidence for a Ping-Pong model for piRNA amplification in C. elegans. Instead, we demonstrate that Piwi acts upstream of an endogenous siRNA pathway in Tc3 silencing. These data might suggest a link between piRNA and siRNA function.

AB - The Piwi proteins of the Argonaute superfamily are required for normal germline development in Drosophila, zebrafish, and mice and associate with 24-30 nucleotide RNAs termed piRNAs. We identify a class of 21 nucleotide RNAs, previously named 21U-RNAs, as the piRNAs of C. elegans. Piwi and piRNA expression is restricted to the male and female germline and independent of many proteins in other small-RNA pathways, including DCR-1. We show that Piwi is specifically required to silence Tc3, but not other Tc/mariner DNA transposons. Tc3 excision rates in the germline are increased at least 100-fold in piwi mutants as compared to wild-type. We find no evidence for a Ping-Pong model for piRNA amplification in C. elegans. Instead, we demonstrate that Piwi acts upstream of an endogenous siRNA pathway in Tc3 silencing. These data might suggest a link between piRNA and siRNA function.

KW - DEVBIO

KW - RNA

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DO - 10.1016/j.molcel.2008.06.003

M3 - Article

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AN - SCOPUS:46149092009

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EP - 90

JO - Molecular Cell

JF - Molecular Cell

SN - 1097-2765

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