PiRNAs and Aubergine cooperate with Wispy poly(A) polymerase to stabilize mRNAs in the germ plasm

Jérémy Dufourt, Gwénaëlle Bontonou, Aymeric Chartier, Camille Jahan, Anne Cécile Meunier, Stéphanie Pierson, Paul F. Harrison, Catherine Papin, Traude H. Beilharz, Martine Simonelig

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Piwi-interacting RNAs (piRNAs) and PIWI proteins play a crucial role in germ cells by repressing transposable elements and regulating gene expression. In Drosophila, maternal piRNAs are loaded into the embryo mostly bound to the PIWI protein Aubergine (Aub). Aub targets maternal mRNAs through incomplete base-pairing with piRNAs and can induce their destabilization in the somatic part of the embryo. Paradoxically, these Aub-dependent unstable mRNAs encode germ cell determinants that are selectively stabilized in the germ plasm. Here we show that piRNAs and Aub actively protect germ cell mRNAs in the germ plasm. Aub directly interacts with the germline-specific poly(A) polymerase Wispy, thus leading to mRNA polyadenylation and stabilization in the germ plasm. These results reveal a role for piRNAs in mRNA stabilization and identify Aub as an interactor of Wispy for mRNA polyadenylation. They further highlight the role of Aub and piRNAs in embryonic patterning through two opposite functions.

Original languageEnglish
Article number1305
Number of pages12
JournalNature Communications
Volume8
Issue number1
DOIs
Publication statusPublished - 1 Dec 2017

Keywords

  • oogenesis
  • piwi RNAs

Cite this

Dufourt, J., Bontonou, G., Chartier, A., Jahan, C., Meunier, A. C., Pierson, S., ... Simonelig, M. (2017). PiRNAs and Aubergine cooperate with Wispy poly(A) polymerase to stabilize mRNAs in the germ plasm. Nature Communications, 8(1), [1305]. https://doi.org/10.1038/s41467-017-01431-5
Dufourt, Jérémy ; Bontonou, Gwénaëlle ; Chartier, Aymeric ; Jahan, Camille ; Meunier, Anne Cécile ; Pierson, Stéphanie ; Harrison, Paul F. ; Papin, Catherine ; Beilharz, Traude H. ; Simonelig, Martine. / PiRNAs and Aubergine cooperate with Wispy poly(A) polymerase to stabilize mRNAs in the germ plasm. In: Nature Communications. 2017 ; Vol. 8, No. 1.
@article{fc62c3a70b97422986a2e2d30f390215,
title = "PiRNAs and Aubergine cooperate with Wispy poly(A) polymerase to stabilize mRNAs in the germ plasm",
abstract = "Piwi-interacting RNAs (piRNAs) and PIWI proteins play a crucial role in germ cells by repressing transposable elements and regulating gene expression. In Drosophila, maternal piRNAs are loaded into the embryo mostly bound to the PIWI protein Aubergine (Aub). Aub targets maternal mRNAs through incomplete base-pairing with piRNAs and can induce their destabilization in the somatic part of the embryo. Paradoxically, these Aub-dependent unstable mRNAs encode germ cell determinants that are selectively stabilized in the germ plasm. Here we show that piRNAs and Aub actively protect germ cell mRNAs in the germ plasm. Aub directly interacts with the germline-specific poly(A) polymerase Wispy, thus leading to mRNA polyadenylation and stabilization in the germ plasm. These results reveal a role for piRNAs in mRNA stabilization and identify Aub as an interactor of Wispy for mRNA polyadenylation. They further highlight the role of Aub and piRNAs in embryonic patterning through two opposite functions.",
keywords = "oogenesis, piwi RNAs",
author = "J{\'e}r{\'e}my Dufourt and Gw{\'e}na{\"e}lle Bontonou and Aymeric Chartier and Camille Jahan and Meunier, {Anne C{\'e}cile} and St{\'e}phanie Pierson and Harrison, {Paul F.} and Catherine Papin and Beilharz, {Traude H.} and Martine Simonelig",
year = "2017",
month = "12",
day = "1",
doi = "10.1038/s41467-017-01431-5",
language = "English",
volume = "8",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "Nature Publishing Group",
number = "1",

}

Dufourt, J, Bontonou, G, Chartier, A, Jahan, C, Meunier, AC, Pierson, S, Harrison, PF, Papin, C, Beilharz, TH & Simonelig, M 2017, 'PiRNAs and Aubergine cooperate with Wispy poly(A) polymerase to stabilize mRNAs in the germ plasm' Nature Communications, vol. 8, no. 1, 1305. https://doi.org/10.1038/s41467-017-01431-5

PiRNAs and Aubergine cooperate with Wispy poly(A) polymerase to stabilize mRNAs in the germ plasm. / Dufourt, Jérémy; Bontonou, Gwénaëlle; Chartier, Aymeric; Jahan, Camille; Meunier, Anne Cécile; Pierson, Stéphanie; Harrison, Paul F.; Papin, Catherine; Beilharz, Traude H.; Simonelig, Martine.

In: Nature Communications, Vol. 8, No. 1, 1305, 01.12.2017.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - PiRNAs and Aubergine cooperate with Wispy poly(A) polymerase to stabilize mRNAs in the germ plasm

AU - Dufourt, Jérémy

AU - Bontonou, Gwénaëlle

AU - Chartier, Aymeric

AU - Jahan, Camille

AU - Meunier, Anne Cécile

AU - Pierson, Stéphanie

AU - Harrison, Paul F.

AU - Papin, Catherine

AU - Beilharz, Traude H.

AU - Simonelig, Martine

PY - 2017/12/1

Y1 - 2017/12/1

N2 - Piwi-interacting RNAs (piRNAs) and PIWI proteins play a crucial role in germ cells by repressing transposable elements and regulating gene expression. In Drosophila, maternal piRNAs are loaded into the embryo mostly bound to the PIWI protein Aubergine (Aub). Aub targets maternal mRNAs through incomplete base-pairing with piRNAs and can induce their destabilization in the somatic part of the embryo. Paradoxically, these Aub-dependent unstable mRNAs encode germ cell determinants that are selectively stabilized in the germ plasm. Here we show that piRNAs and Aub actively protect germ cell mRNAs in the germ plasm. Aub directly interacts with the germline-specific poly(A) polymerase Wispy, thus leading to mRNA polyadenylation and stabilization in the germ plasm. These results reveal a role for piRNAs in mRNA stabilization and identify Aub as an interactor of Wispy for mRNA polyadenylation. They further highlight the role of Aub and piRNAs in embryonic patterning through two opposite functions.

AB - Piwi-interacting RNAs (piRNAs) and PIWI proteins play a crucial role in germ cells by repressing transposable elements and regulating gene expression. In Drosophila, maternal piRNAs are loaded into the embryo mostly bound to the PIWI protein Aubergine (Aub). Aub targets maternal mRNAs through incomplete base-pairing with piRNAs and can induce their destabilization in the somatic part of the embryo. Paradoxically, these Aub-dependent unstable mRNAs encode germ cell determinants that are selectively stabilized in the germ plasm. Here we show that piRNAs and Aub actively protect germ cell mRNAs in the germ plasm. Aub directly interacts with the germline-specific poly(A) polymerase Wispy, thus leading to mRNA polyadenylation and stabilization in the germ plasm. These results reveal a role for piRNAs in mRNA stabilization and identify Aub as an interactor of Wispy for mRNA polyadenylation. They further highlight the role of Aub and piRNAs in embryonic patterning through two opposite functions.

KW - oogenesis

KW - piwi RNAs

UR - http://www.scopus.com/inward/record.url?scp=85032817103&partnerID=8YFLogxK

U2 - 10.1038/s41467-017-01431-5

DO - 10.1038/s41467-017-01431-5

M3 - Article

VL - 8

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

IS - 1

M1 - 1305

ER -