PI(3,4,5)P3 interactome

Bruno Catimel, Meng -Xin Yin, Christine Schieber, Melanie Condron, Heather Patsiouras, Jenny Catimel, Diane E J E Robinson, Leon S -M Wong, Edouard C Nice, Andrew B Holmes, Antony W Burgess

Research output: Contribution to journalArticleResearchpeer-review

36 Citations (Scopus)

Abstract

Immobilizing chemically synthesized analogues of PI(3,4,5)P3 onto Affi-10 beads and incorporating them into liposomes allowed their use as affinity absorbents in the comprehensive analysis of the phosphoinositide interactome using cytosolic cell extracts of the LIM1215 colon cancer cell line. This led to the identification of 282 proteins that either interact with PI(3,4,5)P3 or are indirectly captured as part of a complex containing a PI(3,4,5)P3 binding partner. Identification of the proteins was achieved using affinity/LC-MS/MS experiments.
Original languageEnglish
Pages (from-to)3712 - 3726
Number of pages15
JournalJournal of Proteome Research
Volume8
Issue number7
Publication statusPublished - 2009
Externally publishedYes

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