Phytochemical analysis and in vitro evaluation of antidiabetic activity of Diospyros buxifolia

Pasupuleti Visweswara Rao, Elawarasi Krishinasamy, Indireddy Rama Manohar Reddy, Malepati Dhananjaya Naidu, Siew Hua Gan

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Abstract

Background: Diabetes mellitus is a chronic disease and is one of the main public health concerns worldwide. To date, the potential of many natural products in its treatment are not fully explored. Materials and Methods: In this study, major phytochemicals present in the leaves and stems of Diospyros buxifolia were determined following extraction using distilled water and 70% of methanol. The antidiabetic activities of the extracts were determined in vitro by measuring α-amylase and amyloglucosidase inhibitory activities at four different extract concentrations (0.125, 0.250, 0.500 and 1.000 mg mL-1). Results: Methanolic extracts of D. buxifolia stems found to possess significant α-amylase inhibitory activity (84.68±0.03%) followed by methanolic extract of the leaves (84.00±0.08%), aqueous extracts of the stems (49.44±0.05%) and aqueous extract of the leaves (40.71±0.03%). Methanolic extracts of the stems showed significant inhibition of amyloglucosidase (62.72±0.15%) followed by methanolic extract of the leaves (58.93±0.08%), aqueous extracts of the stems (36.65±0.05%) and finally aqueous extract of the leaves (20.57±0.03%). Conclusion: It can be concluded that methanolic stem extract of D. buxifolia displayed the presence of phytochemicals and also significant a-amylase and amyloglucosidase inhibition. Further analysis needs to be made to find out the mechanism of action of antidiabetic activities of the extracts used in this study.

Original languageEnglish
Pages (from-to)95-101
Number of pages7
JournalAmerican Journal of Biochemistry and Molecular Biology
Volume6
Issue number3-4
DOIs
Publication statusPublished - 15 Sep 2016
Externally publishedYes

Keywords

  • Diabetes
  • Diaspyros buxifolia
  • Medicinal plants
  • α-amylase

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