Background: Diabetes mellitus is a chronic disease and is one of the main public health concerns worldwide. To date, the potential of many natural products in its treatment are not fully explored. Materials and Methods: In this study, major phytochemicals present in the leaves and stems of Diospyros buxifolia were determined following extraction using distilled water and 70% of methanol. The antidiabetic activities of the extracts were determined in vitro by measuring α-amylase and amyloglucosidase inhibitory activities at four different extract concentrations (0.125, 0.250, 0.500 and 1.000 mg mL-1). Results: Methanolic extracts of D. buxifolia stems found to possess significant α-amylase inhibitory activity (84.68±0.03%) followed by methanolic extract of the leaves (84.00±0.08%), aqueous extracts of the stems (49.44±0.05%) and aqueous extract of the leaves (40.71±0.03%). Methanolic extracts of the stems showed significant inhibition of amyloglucosidase (62.72±0.15%) followed by methanolic extract of the leaves (58.93±0.08%), aqueous extracts of the stems (36.65±0.05%) and finally aqueous extract of the leaves (20.57±0.03%). Conclusion: It can be concluded that methanolic stem extract of D. buxifolia displayed the presence of phytochemicals and also significant a-amylase and amyloglucosidase inhibition. Further analysis needs to be made to find out the mechanism of action of antidiabetic activities of the extracts used in this study.
|Number of pages||7|
|Journal||American Journal of Biochemistry and Molecular Biology|
|Publication status||Published - 15 Sep 2016|
- Diaspyros buxifolia
- Medicinal plants