TY - JOUR
T1 - Physico-chemical characterization and tissue healing changes by Hancornia speciosa Gomes latex biomembrane
AU - Pegorin, Giovana S.
AU - Leite, Marcel N.
AU - Antoniassi, Marcio
AU - Chagas, Ana Laura D.
AU - Santana, Luísiane A.
AU - Garms, Bruna C.
AU - Marcelino, Mônica Y.
AU - Herculano, Rondinelli D.
AU - Cipriani Frade, Marco Andrey
N1 - Funding Information:
Conselho Nacional de Desenvolvimento Científico e Tecnológico, Grant/Award Number: 470261/2012; Fundação de Amparo à Pesquisa do Estado de São Paulo, Grant/Award Numbers: 2009/09355‐0, 2016/16437‐7, 2017/19603‐8 Funding information
Funding Information:
This work was supported by a grant from the PIBIC/CNPq, Coordination for the Improvement of Higher Education Personnel (CAPES), FAPESP (Processes 2009/09355-0, 2016/16437-7, 2017/19603-8) and CNPq (Project 470261/2012).
Funding Information:
This work was supported by a grant from the PIBIC/CNPq, Coordination for the Improvement of Higher Education Personnel (CAPES), FAPESP (Processes 2009/09355‐0, 2016/16437‐7, 2017/19603‐8) and CNPq (Project 470261/2012).
Publisher Copyright:
© 2020 Wiley Periodicals LLC.
PY - 2021/7
Y1 - 2021/7
N2 - Skin wounds have been a public health concern of high frequency, in addition to requiring intensive and expensive care. The natural rubber latex (NRL) from Hancornia speciosa Gomes has been used to treat many problems in traditional medicine and also present healing properties, antifungal and anti-inflammatory activity and antinociceptive effects. The purpose of this study was to characterize the new biomembrane from the NRL of H. speciosa (HS) by Fourier transform infrared (FTIR) and mechanical strength test and to investigate its biological properties by the cytotoxicity assay and in vivo healing activity. The results showed that the HS biomembrane exhibited characteristic bands of the main component cis-1,4-polyisoprene. Besides, its Young modulus was close to human skin with adhesive-compatible mechanical characteristics. The cytotoxicity assays revealed that the HS biomembrane was not toxic to fibroblast cells neither using agar diffusion test nor MTT assay. Furthermore, the HS biomembrane stimulated the inflammatory cells and the angiogenesis, increased significantly the collagenesis and improved the quality of heal until the remodeling phase induced by implants in mice. Thus, this biomembrane has proven to be a safe and biocompatible biomaterial with healing potential, becoming an effective and low-cost alternative for the treatment of skin wounds.
AB - Skin wounds have been a public health concern of high frequency, in addition to requiring intensive and expensive care. The natural rubber latex (NRL) from Hancornia speciosa Gomes has been used to treat many problems in traditional medicine and also present healing properties, antifungal and anti-inflammatory activity and antinociceptive effects. The purpose of this study was to characterize the new biomembrane from the NRL of H. speciosa (HS) by Fourier transform infrared (FTIR) and mechanical strength test and to investigate its biological properties by the cytotoxicity assay and in vivo healing activity. The results showed that the HS biomembrane exhibited characteristic bands of the main component cis-1,4-polyisoprene. Besides, its Young modulus was close to human skin with adhesive-compatible mechanical characteristics. The cytotoxicity assays revealed that the HS biomembrane was not toxic to fibroblast cells neither using agar diffusion test nor MTT assay. Furthermore, the HS biomembrane stimulated the inflammatory cells and the angiogenesis, increased significantly the collagenesis and improved the quality of heal until the remodeling phase induced by implants in mice. Thus, this biomembrane has proven to be a safe and biocompatible biomaterial with healing potential, becoming an effective and low-cost alternative for the treatment of skin wounds.
KW - Hancornia speciosa Gomes
KW - latex biomembrane
KW - subcutaneous implants
KW - wound healing
UR - http://www.scopus.com/inward/record.url?scp=85096802383&partnerID=8YFLogxK
U2 - 10.1002/jbm.b.34758
DO - 10.1002/jbm.b.34758
M3 - Article
C2 - 33241610
AN - SCOPUS:85096802383
VL - 109
SP - 938
EP - 948
JO - Journal of Biomedical Materials Research Part B: Applied Biomaterials
JF - Journal of Biomedical Materials Research Part B: Applied Biomaterials
SN - 1552-4973
IS - 7
ER -