@article{3645a73734464fdd8e12884e2b61ab34,
title = "Physical stability and rheological behavior of Pickering emulsions stabilized by protein-polysaccharide hybrid nanoconjugates",
abstract = "This study investigated the emulsifying properties of a protein-polysaccharide hybrid nanoconjugate system comprising cellulose nanocrystals (CNC, 1% w/v) and soy protein isolate at various concentrations (SPI, 1-3% w/v). The average particle size of the nanoconjugate increased, and the zeta potential decreased when 3% (w/v) of SPI was used. The contact angle and thermal stability of CNC improved with the conjugation of SPI. Upon Pickering emulsification, 0.5% (w/v) of CNC-SPI nanoconjugate as particle stabilizer was sufficient to obtain stable emulsions. The CNC-SPI1 formulation (CNC to SPI, 1:1) provided the emulsion with the smallest droplet size and higher emulsifying activity. Intriguingly, ultrasound (US) pre-treatment on nanoconjugates before emulsification significantly reduced the size of the emulsion. The rheological assessment demonstrated that the CNC-SPI-stabilized emulsions exhibit shear thinning behavior at a lower shear rate and shear thickening behavior at a higher shear rate, indicating the interruption of existing attractive interactions between the CNC particles. All emulsions exhibited higher elastic modulus (G′) than viscous modulus (G″), suggesting high viscoelastic properties of the emulsions. This study demonstrates that CNC-SPI nanoconjugate with optimum protein to polysaccharide ratio has great potential as a natural particle stabilizer in food and nutraceutical emulsion applications. ",
keywords = "nanoconjugate, Pickering emulsion, protein-polysaccharide, rheology, stability",
author = "Wong, {See Kiat} and Low, {Liang Ee} and Janarthanan Supramaniam and Sivakumar Manickam and Wong, {Tin Wui} and Pang, {Cheng Heng} and Tang, {Siah Ying}",
note = "Funding Information: Funding information: The S&T Innovation 2025 Major Special Programme (grant number 2018B10022) and the Ningbo Natural Science Foundation Programme (grant number 2018A610069) funded by the Ningbo Science and Technology Bureau, China, as well as the UNNC FoSE Faculty Inspiration Grant, China. The Zhejiang Provincial Department of Science and Technology is also acknowledged for this research under its Provincial Key Laboratory Programme (2020E10018). This project was supported by the Tropical Medicine and Biology Platform, School of Science, Monash University Malaysia. Funding Information: The authors gratefully express gratitude to all parties who have contributed towards the success of this project, both financially and technically, especially the S&T Innovation 2025 Major Special Programme (grant number 2018B10022) and the Ningbo Natural Science Foundation Programme (grant number 2018A610069) funded by the Ningbo Science and Technology Bureau, China, as well as the UNNC FoSE Faculty Inspiration Grant, China. The Zhejiang Provincial Department of Science and Technology is also acknowledged for this research under its Provincial Key Laboratory Programme (2020E10018). This project was supported by the Tropical Medicine and Biology Platform, School of Science, Monash University Malaysia. Publisher Copyright: {\textcopyright} 2021 See Kiat Wong et al., published by De Gruyter.",
year = "2021",
month = jan,
doi = "10.1515/ntrev-2021-0090",
language = "English",
volume = "10",
pages = "1293--1305",
journal = "Nanotechnology Reviews",
issn = "2191-9089",
publisher = "Walter de Gruyter",
number = "1",
}