Photothermal incubation of red blood cells by laser for rapid pre-transfusion blood group typing

Clare A. Manderson, Heather McLiesh, Rodrigo Curvello, Rico F. Tabor, Jim Manolios, Gil Garnier

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Safe blood transfusion requires compatibility testing of donor and recipient to prevent potentially fatal transfusion reactions. Detection of immunoglobulin G (IgG) antibodies requires incubation at 37 °C, often for up to 15 minutes. Current incubation technology predominantly relies on slow thermal-gradient dependent conduction. Here, we present rapid optical heating via laser, where targeted illumination of a blood-antibody sample in a diagnostic gel card is converted into heat, via photothermal absorption. Our laser-incubator heats the 75 µL blood-antibody sample to 37 °C in under 30 seconds. We show that red blood cells act as photothermal agents under near-infrared laser incubation, triggering rapid antigen-antibody binding. We detect no significant damage to the cells or antibodies for laser incubations of up to fifteen minutes. We demonstrate laser-incubated immunohaematological testing to be both faster and more sensitive than current best practice - with clearly positive results seen from laser incubations of just 40 seconds.

Original languageEnglish
Number of pages10
JournalScientific Reports
Volume9
Issue number1
DOIs
Publication statusPublished - 2 Aug 2019

Cite this

@article{c6c9f22870bd40eda90b831f46219627,
title = "Photothermal incubation of red blood cells by laser for rapid pre-transfusion blood group typing",
abstract = "Safe blood transfusion requires compatibility testing of donor and recipient to prevent potentially fatal transfusion reactions. Detection of immunoglobulin G (IgG) antibodies requires incubation at 37 °C, often for up to 15 minutes. Current incubation technology predominantly relies on slow thermal-gradient dependent conduction. Here, we present rapid optical heating via laser, where targeted illumination of a blood-antibody sample in a diagnostic gel card is converted into heat, via photothermal absorption. Our laser-incubator heats the 75 µL blood-antibody sample to 37 °C in under 30 seconds. We show that red blood cells act as photothermal agents under near-infrared laser incubation, triggering rapid antigen-antibody binding. We detect no significant damage to the cells or antibodies for laser incubations of up to fifteen minutes. We demonstrate laser-incubated immunohaematological testing to be both faster and more sensitive than current best practice - with clearly positive results seen from laser incubations of just 40 seconds.",
author = "Manderson, {Clare A.} and Heather McLiesh and Rodrigo Curvello and Tabor, {Rico F.} and Jim Manolios and Gil Garnier",
year = "2019",
month = "8",
day = "2",
doi = "10.1038/s41598-019-47646-y",
language = "English",
volume = "9",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
number = "1",

}

Photothermal incubation of red blood cells by laser for rapid pre-transfusion blood group typing. / Manderson, Clare A.; McLiesh, Heather; Curvello, Rodrigo; Tabor, Rico F.; Manolios, Jim; Garnier, Gil.

In: Scientific Reports, Vol. 9, No. 1, 02.08.2019.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Photothermal incubation of red blood cells by laser for rapid pre-transfusion blood group typing

AU - Manderson, Clare A.

AU - McLiesh, Heather

AU - Curvello, Rodrigo

AU - Tabor, Rico F.

AU - Manolios, Jim

AU - Garnier, Gil

PY - 2019/8/2

Y1 - 2019/8/2

N2 - Safe blood transfusion requires compatibility testing of donor and recipient to prevent potentially fatal transfusion reactions. Detection of immunoglobulin G (IgG) antibodies requires incubation at 37 °C, often for up to 15 minutes. Current incubation technology predominantly relies on slow thermal-gradient dependent conduction. Here, we present rapid optical heating via laser, where targeted illumination of a blood-antibody sample in a diagnostic gel card is converted into heat, via photothermal absorption. Our laser-incubator heats the 75 µL blood-antibody sample to 37 °C in under 30 seconds. We show that red blood cells act as photothermal agents under near-infrared laser incubation, triggering rapid antigen-antibody binding. We detect no significant damage to the cells or antibodies for laser incubations of up to fifteen minutes. We demonstrate laser-incubated immunohaematological testing to be both faster and more sensitive than current best practice - with clearly positive results seen from laser incubations of just 40 seconds.

AB - Safe blood transfusion requires compatibility testing of donor and recipient to prevent potentially fatal transfusion reactions. Detection of immunoglobulin G (IgG) antibodies requires incubation at 37 °C, often for up to 15 minutes. Current incubation technology predominantly relies on slow thermal-gradient dependent conduction. Here, we present rapid optical heating via laser, where targeted illumination of a blood-antibody sample in a diagnostic gel card is converted into heat, via photothermal absorption. Our laser-incubator heats the 75 µL blood-antibody sample to 37 °C in under 30 seconds. We show that red blood cells act as photothermal agents under near-infrared laser incubation, triggering rapid antigen-antibody binding. We detect no significant damage to the cells or antibodies for laser incubations of up to fifteen minutes. We demonstrate laser-incubated immunohaematological testing to be both faster and more sensitive than current best practice - with clearly positive results seen from laser incubations of just 40 seconds.

UR - http://www.scopus.com/inward/record.url?scp=85071195568&partnerID=8YFLogxK

U2 - 10.1038/s41598-019-47646-y

DO - 10.1038/s41598-019-47646-y

M3 - Article

VL - 9

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

IS - 1

ER -