Photonic bioreactors based on multiple internal reflection

Bergoi Ibarlucea, Victor Javier Cadarso, Jahir Orozco, Stefanie Demming, Cesar Fernández-Sánchez, Ralph Wilke, Stephanus Büttgenbach, Andreu Llobera, Andreu Llobera

Research output: Chapter in Book/Report/Conference proceedingConference PaperResearchpeer-review

Abstract

Propagating Multiple Internal Reflection (PMIR) systems have been functionalized with horseradish peroxidase (HRP) so as to obtain highly sensitive PDMS-based photonic bioreactors in which photonic, microfluidcs and recognition elements are merged in a single lab on a chip. Demonstration of the working principle was done by making absorbance measurements in ABTS solutions with increasing hydrogen peroxide concentrations, which allowed determining a sensitivity of 0.025±0.004 (a.u.)/μM and a LOD of 2.8±0.4 μM.

Original languageEnglish
Title of host publicationProceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages287-289
Number of pages3
ISBN (Print)9780979806421
Publication statusPublished - 2009
EventInternational Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2009) - Jeju, Korea, Republic of (South)
Duration: 1 Nov 20095 Nov 2009
Conference number: 13th

Conference

ConferenceInternational Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2009)
Abbreviated titleMicroTAS 2009
CountryKorea, Republic of (South)
CityJeju
Period1/11/095/11/09

Keywords

  • Biofunctionalization
  • PDMS
  • Photonic lab-on-a-chip
  • PMIR

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