Photonic bioreactors based on multiple internal reflection

Bergoi Ibarlucea; Victor Javier Cadarso; Jahir Orozco; Stefanie Demming; Cesar Fernández-Sánchez; Ralph Wilke; Stephanus Büttgenbach; Andreu Llobera; Andreu Llobera

Photonic bioreactors based on multiple internal reflection

Bergoi Ibarlucea, Victor Javier Cadarso, Jahir Orozco, Stefanie Demming, Cesar Fernández-Sánchez, Ralph Wilke, Stephanus Büttgenbach, Andreu Llobera, Andreu Llobera

Mechanical & Aerospace Engineering

Research output: Chapter in Book/Report/Conference proceeding › Conference Paper › Research › peer-review

Abstract

Propagating Multiple Internal Reflection (PMIR) systems have been functionalized with horseradish peroxidase (HRP) so as to obtain highly sensitive PDMS-based photonic bioreactors in which photonic, microfluidcs and recognition elements are merged in a single lab on a chip. Demonstration of the working principle was done by making absorbance measurements in ABTS solutions with increasing hydrogen peroxide concentrations, which allowed determining a sensitivity of 0.025±0.004 (a.u.)/μM and a LOD of 2.8±0.4 μM.

Original language	English
Title of host publication	Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Publisher	Chemical and Biological Microsystems Society
Pages	287-289
Number of pages	3
ISBN (Print)	9780979806421
Publication status	Published - 2009
Event	International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2009) - Jeju, Korea, Republic of (South) Duration: 1 Nov 2009 → 5 Nov 2009 Conference number: 13th

Conference

Conference	International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2009)
Abbreviated title	MicroTAS 2009
Country/Territory	Korea, Republic of (South)
City	Jeju
Period	1/11/09 → 5/11/09

Keywords

Biofunctionalization
PDMS
Photonic lab-on-a-chip
PMIR

Cite this

Ibarlucea, B., Cadarso, V. J., Orozco, J., Demming, S., Fernández-Sánchez, C., Wilke, R., Büttgenbach, S., Llobera, A., & Llobera, A. (2009). Photonic bioreactors based on multiple internal reflection. In Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 287-289). Chemical and Biological Microsystems Society.

Ibarlucea, Bergoi ; Cadarso, Victor Javier ; Orozco, Jahir ; Demming, Stefanie ; Fernández-Sánchez, Cesar ; Wilke, Ralph ; Büttgenbach, Stephanus ; Llobera, Andreu ; Llobera, Andreu. / Photonic bioreactors based on multiple internal reflection. Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2009. pp. 287-289

@inproceedings{df1a45ec69974420b8809e298a8b9088,

title = "Photonic bioreactors based on multiple internal reflection",

abstract = "Propagating Multiple Internal Reflection (PMIR) systems have been functionalized with horseradish peroxidase (HRP) so as to obtain highly sensitive PDMS-based photonic bioreactors in which photonic, microfluidcs and recognition elements are merged in a single lab on a chip. Demonstration of the working principle was done by making absorbance measurements in ABTS solutions with increasing hydrogen peroxide concentrations, which allowed determining a sensitivity of 0.025±0.004 (a.u.)/μM and a LOD of 2.8±0.4 μM.",

keywords = "Biofunctionalization, PDMS, Photonic lab-on-a-chip, PMIR",

author = "Bergoi Ibarlucea and Cadarso, {Victor Javier} and Jahir Orozco and Stefanie Demming and Cesar Fern{\'a}ndez-S{\'a}nchez and Ralph Wilke and Stephanus B{\"u}ttgenbach and Andreu Llobera and Andreu Llobera",

year = "2009",

language = "English",

isbn = "9780979806421",

pages = "287--289",

booktitle = "Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences",

publisher = "Chemical and Biological Microsystems Society",

address = "United States of America",

note = "International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2009), MicroTAS 2009 ; Conference date: 01-11-2009 Through 05-11-2009",

}

Ibarlucea, B, Cadarso, VJ, Orozco, J, Demming, S, Fernández-Sánchez, C, Wilke, R, Büttgenbach, S, Llobera, A & Llobera, A 2009, Photonic bioreactors based on multiple internal reflection. in Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, pp. 287-289, International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2009), Jeju, Korea, Republic of (South), 1/11/09.

Photonic bioreactors based on multiple internal reflection. / Ibarlucea, Bergoi; Cadarso, Victor Javier; Orozco, Jahir; Demming, Stefanie; Fernández-Sánchez, Cesar; Wilke, Ralph; Büttgenbach, Stephanus; Llobera, Andreu; Llobera, Andreu.

Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2009. p. 287-289.

Research output: Chapter in Book/Report/Conference proceeding › Conference Paper › Research › peer-review

TY - GEN

T1 - Photonic bioreactors based on multiple internal reflection

AU - Ibarlucea, Bergoi

AU - Cadarso, Victor Javier

AU - Orozco, Jahir

AU - Demming, Stefanie

AU - Fernández-Sánchez, Cesar

AU - Wilke, Ralph

AU - Büttgenbach, Stephanus

AU - Llobera, Andreu

N1 - Conference code: 13th

PY - 2009

Y1 - 2009

N2 - Propagating Multiple Internal Reflection (PMIR) systems have been functionalized with horseradish peroxidase (HRP) so as to obtain highly sensitive PDMS-based photonic bioreactors in which photonic, microfluidcs and recognition elements are merged in a single lab on a chip. Demonstration of the working principle was done by making absorbance measurements in ABTS solutions with increasing hydrogen peroxide concentrations, which allowed determining a sensitivity of 0.025±0.004 (a.u.)/μM and a LOD of 2.8±0.4 μM.

AB - Propagating Multiple Internal Reflection (PMIR) systems have been functionalized with horseradish peroxidase (HRP) so as to obtain highly sensitive PDMS-based photonic bioreactors in which photonic, microfluidcs and recognition elements are merged in a single lab on a chip. Demonstration of the working principle was done by making absorbance measurements in ABTS solutions with increasing hydrogen peroxide concentrations, which allowed determining a sensitivity of 0.025±0.004 (a.u.)/μM and a LOD of 2.8±0.4 μM.

KW - Biofunctionalization

KW - PDMS

KW - Photonic lab-on-a-chip

KW - PMIR

UR - http://www.scopus.com/inward/record.url?scp=84901773608&partnerID=8YFLogxK

M3 - Conference Paper

AN - SCOPUS:84901773608

SN - 9780979806421

SP - 287

EP - 289

BT - Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences

PB - Chemical and Biological Microsystems Society

T2 - International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2009)

Y2 - 1 November 2009 through 5 November 2009

ER -

Photonic bioreactors based on multiple internal reflection

Abstract

Conference

Keywords

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