Photodegradable gelatin-based hydrogels prepared by bioorthogonal click chemistry for cell encapsulation and release

Vinh X. Truong; Kelly M. Tsang; George P. Simon; Richard L. Boyd; Richard A. Evans; Helmut Thissen; John S. Forsythe

doi:10.1021/acs.biomac.5b00706

Photodegradable gelatin-based hydrogels prepared by bioorthogonal click chemistry for cell encapsulation and release

Vinh X. Truong, Kelly M. Tsang, George P. Simon, Richard L. Boyd, Richard A. Evans, Helmut Thissen, John S. Forsythe

Research output: Contribution to journal › Article › Research › peer-review

85 Citations (Scopus)

Abstract

In this study, we present a method for the fabrication of in situ forming gelatin and poly(ethylene glycol)- based hydrogels utilizing bioorthogonal, strain-promoted alkyne−azide cycloaddition as the cross-linking reaction. By incorporating nitrobenzyl moieties within the network structure, these hydrogels can be designed to be degradable upon irradiation with low intensity UV light, allowing precise photopatterning. Fibroblast cells encapsulated within these hydrogels were viable at 14 days and could be readily harvested using a light trigger. Potential applications of this new class of injectable hydrogel include its use as a 3D culturing platform that allows the capture and release of cells, as well as light-triggered cell delivery in regenerative medicine.

Original language	English
Pages (from-to)	2246 - 2253
Number of pages	8
Journal	Biomacromolecules
Volume	16
Issue number	7
DOIs	https://doi.org/10.1021/acs.biomac.5b00706
Publication status	Published - 2015

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title = "Photodegradable gelatin-based hydrogels prepared by bioorthogonal click chemistry for cell encapsulation and release",

abstract = "In this study, we present a method for the fabrication of in situ forming gelatin and poly(ethylene glycol)- based hydrogels utilizing bioorthogonal, strain-promoted alkyne−azide cycloaddition as the cross-linking reaction. By incorporating nitrobenzyl moieties within the network structure, these hydrogels can be designed to be degradable upon irradiation with low intensity UV light, allowing precise photopatterning. Fibroblast cells encapsulated within these hydrogels were viable at 14 days and could be readily harvested using a light trigger. Potential applications of this new class of injectable hydrogel include its use as a 3D culturing platform that allows the capture and release of cells, as well as light-triggered cell delivery in regenerative medicine.",

author = "Truong, {Vinh X.} and Tsang, {Kelly M.} and Simon, {George P.} and Boyd, {Richard L.} and Evans, {Richard A.} and Helmut Thissen and Forsythe, {John S.}",

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T1 - Photodegradable gelatin-based hydrogels prepared by bioorthogonal click chemistry for cell encapsulation and release

AU - Truong, Vinh X.

AU - Tsang, Kelly M.

AU - Simon, George P.

AU - Boyd, Richard L.

AU - Evans, Richard A.

AU - Thissen, Helmut

AU - Forsythe, John S.

PY - 2015

Y1 - 2015

N2 - In this study, we present a method for the fabrication of in situ forming gelatin and poly(ethylene glycol)- based hydrogels utilizing bioorthogonal, strain-promoted alkyne−azide cycloaddition as the cross-linking reaction. By incorporating nitrobenzyl moieties within the network structure, these hydrogels can be designed to be degradable upon irradiation with low intensity UV light, allowing precise photopatterning. Fibroblast cells encapsulated within these hydrogels were viable at 14 days and could be readily harvested using a light trigger. Potential applications of this new class of injectable hydrogel include its use as a 3D culturing platform that allows the capture and release of cells, as well as light-triggered cell delivery in regenerative medicine.

AB - In this study, we present a method for the fabrication of in situ forming gelatin and poly(ethylene glycol)- based hydrogels utilizing bioorthogonal, strain-promoted alkyne−azide cycloaddition as the cross-linking reaction. By incorporating nitrobenzyl moieties within the network structure, these hydrogels can be designed to be degradable upon irradiation with low intensity UV light, allowing precise photopatterning. Fibroblast cells encapsulated within these hydrogels were viable at 14 days and could be readily harvested using a light trigger. Potential applications of this new class of injectable hydrogel include its use as a 3D culturing platform that allows the capture and release of cells, as well as light-triggered cell delivery in regenerative medicine.

UR - http://pubs.acs.org/doi/pdf/10.1021/acs.biomac.5b00706

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DO - 10.1021/acs.biomac.5b00706

M3 - Article

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VL - 16

SP - 2246

EP - 2253

JO - Biomacromolecules

JF - Biomacromolecules

IS - 7

ER -

Photodegradable gelatin-based hydrogels prepared by bioorthogonal click chemistry for cell encapsulation and release

Abstract

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