Photochemically induced dynamic nuclear polarisation NMR study of the aromatic residues of sea‐anemone polypeptide cardiac stimulants

Raymond S. NORTON, Laszlo BERESS, Sijtze STOB, Rolf BOELENS, Robert KAPTEIN

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Abstract

One‐dimensional and two‐dimensional photochemically induced dynamic nuclear polarisation (photo‐CIDNP) nuclear magnetic resonance spectra have been recorded for the sea‐anemone polypeptide cardiac stimulants anthopleurin‐A and Anemonia sulcata toxins I and II. In anthopleurin‐A and toxin II, all three Trp residues are accessible to the flavin dye, although Trp‐23 in anthopleurin‐A shows a weaker photo‐CIDNP response than Trp‐33 and Trp‐45. Tyr‐25 in anthopleurin‐A also shows a strong response. In toxin I, Trp‐23, Trp‐33 and Tyr‐45 (which replaces Trp in this molecule) are accessible to the dye. The pH dependences of the photo‐CIDNP spectra of all three polypeptides have been examined. The response of Trp‐33 increases significantly with pH. The two His residues of anthopleurin‐A and toxin II display a response in their imidazole forms, but not their imidazolium forms. The surface accessibilities of Trp‐23 and Trp‐33 are discussed in relation to the interaction of these polypeptides with the Na+ channel.

Original languageEnglish
Pages (from-to)343-346
Number of pages4
JournalEuropean Journal of Biochemistry
Volume157
Issue number2
DOIs
Publication statusPublished - 1986
Externally publishedYes

Cite this

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title = "Photochemically induced dynamic nuclear polarisation NMR study of the aromatic residues of sea‐anemone polypeptide cardiac stimulants",
abstract = "One‐dimensional and two‐dimensional photochemically induced dynamic nuclear polarisation (photo‐CIDNP) nuclear magnetic resonance spectra have been recorded for the sea‐anemone polypeptide cardiac stimulants anthopleurin‐A and Anemonia sulcata toxins I and II. In anthopleurin‐A and toxin II, all three Trp residues are accessible to the flavin dye, although Trp‐23 in anthopleurin‐A shows a weaker photo‐CIDNP response than Trp‐33 and Trp‐45. Tyr‐25 in anthopleurin‐A also shows a strong response. In toxin I, Trp‐23, Trp‐33 and Tyr‐45 (which replaces Trp in this molecule) are accessible to the dye. The pH dependences of the photo‐CIDNP spectra of all three polypeptides have been examined. The response of Trp‐33 increases significantly with pH. The two His residues of anthopleurin‐A and toxin II display a response in their imidazole forms, but not their imidazolium forms. The surface accessibilities of Trp‐23 and Trp‐33 are discussed in relation to the interaction of these polypeptides with the Na+ channel.",
author = "NORTON, {Raymond S.} and Laszlo BERESS and Sijtze STOB and Rolf BOELENS and Robert KAPTEIN",
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Photochemically induced dynamic nuclear polarisation NMR study of the aromatic residues of sea‐anemone polypeptide cardiac stimulants. / NORTON, Raymond S.; BERESS, Laszlo; STOB, Sijtze; BOELENS, Rolf; KAPTEIN, Robert.

In: European Journal of Biochemistry, Vol. 157, No. 2, 1986, p. 343-346.

Research output: Contribution to journalArticleResearchpeer-review

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T1 - Photochemically induced dynamic nuclear polarisation NMR study of the aromatic residues of sea‐anemone polypeptide cardiac stimulants

AU - NORTON, Raymond S.

AU - BERESS, Laszlo

AU - STOB, Sijtze

AU - BOELENS, Rolf

AU - KAPTEIN, Robert

PY - 1986

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N2 - One‐dimensional and two‐dimensional photochemically induced dynamic nuclear polarisation (photo‐CIDNP) nuclear magnetic resonance spectra have been recorded for the sea‐anemone polypeptide cardiac stimulants anthopleurin‐A and Anemonia sulcata toxins I and II. In anthopleurin‐A and toxin II, all three Trp residues are accessible to the flavin dye, although Trp‐23 in anthopleurin‐A shows a weaker photo‐CIDNP response than Trp‐33 and Trp‐45. Tyr‐25 in anthopleurin‐A also shows a strong response. In toxin I, Trp‐23, Trp‐33 and Tyr‐45 (which replaces Trp in this molecule) are accessible to the dye. The pH dependences of the photo‐CIDNP spectra of all three polypeptides have been examined. The response of Trp‐33 increases significantly with pH. The two His residues of anthopleurin‐A and toxin II display a response in their imidazole forms, but not their imidazolium forms. The surface accessibilities of Trp‐23 and Trp‐33 are discussed in relation to the interaction of these polypeptides with the Na+ channel.

AB - One‐dimensional and two‐dimensional photochemically induced dynamic nuclear polarisation (photo‐CIDNP) nuclear magnetic resonance spectra have been recorded for the sea‐anemone polypeptide cardiac stimulants anthopleurin‐A and Anemonia sulcata toxins I and II. In anthopleurin‐A and toxin II, all three Trp residues are accessible to the flavin dye, although Trp‐23 in anthopleurin‐A shows a weaker photo‐CIDNP response than Trp‐33 and Trp‐45. Tyr‐25 in anthopleurin‐A also shows a strong response. In toxin I, Trp‐23, Trp‐33 and Tyr‐45 (which replaces Trp in this molecule) are accessible to the dye. The pH dependences of the photo‐CIDNP spectra of all three polypeptides have been examined. The response of Trp‐33 increases significantly with pH. The two His residues of anthopleurin‐A and toxin II display a response in their imidazole forms, but not their imidazolium forms. The surface accessibilities of Trp‐23 and Trp‐33 are discussed in relation to the interaction of these polypeptides with the Na+ channel.

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